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[1]姜洋,杨惠民,马玉琪,等.补肾祛瘀化痰法对载脂蛋白E基因敲除小鼠Morris水迷宫研究的影响[J].环球中医药,2016,9(01):25-28.[doi:10.3969/j.issn.1674-1749.2016.01.006]
　JIANG Yang,YANG Hui-min,MA Yu-qi,et al.The effects of tonifying kidney and removing stasis and phlegm on morris water maze of ApoE knockout mice[J].,2016,9(01):25-28.[doi:10.3969/j.issn.1674-1749.2016.01.006]
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补肾祛瘀化痰法对载脂蛋白E基因敲除小鼠 Morris水迷宫研究的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年01期
页码:
25-28
栏目:
论著
出版日期:
2016-01-06

文章信息/Info

Title:
The effects of tonifying kidney and removing stasis and phlegm on morris water maze of ApoE knockout mice
作者:
姜洋; 杨惠民; 马玉琪; 梁志齐
100035 北京积水潭医院中医科(姜洋、马玉棋、梁志齐);
北京中医药大学第一临床医学院教务处(杨惠民)
Author(s):
JIANG Yang;  YANG Hui-min;  MA Yu-qi;  et al.
Department of traditional Chinese medicine, Beijing Jishuitan Hospital, Beijing 100035, China
关键词:
补肾祛瘀化痰法;  ApoE基因敲除小鼠;  Morris水迷宫
Keywords:
Tonifying kidney and removing stasis and phlegm;  ApoE knockout mice;  Morris water maze
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.01.006
文献标志码:
A
摘要:
目的 通过研究补肾祛瘀化痰法对ApoE基因敲除小鼠Morris水迷宫成绩的影响,探讨补肾祛瘀化痰法对认知功能障碍的改善作用。方法 将ApoE基因敲除小鼠共32只随机分为成年中药组、成年模型组及老年中药组、老年模型组,并取相同遗传背景的C57BL/6J小鼠共16只随机分成成年正常组及老年正常组,连续给予补肾祛瘀化痰法组方灌胃12周,成年组至32周龄、老年组至44周龄时检测Morris水迷宫定位航行实验及空间探索实验的成绩。 结果 定位航行实验逃避潜伏期结果不符合正态分布,故采用非参数检验中K组独立样本检验进行数据统计:成年各组之间逃避潜伏期无明显差异(P>0.05)。老年模型组较老年中药组逃避潜伏期显著延长(P<0.01),老年模型组较老年正常组亦明显延长(P<0.05)。空间探索实验中,第Ⅲ象限路程及第Ⅲ象限时间均符合正态分布,故采用单因素方差分析进行数据统计:成年各组之间在穿台次数、第Ⅲ象限路程及第Ⅲ象限时间均无明显差异(P>0.05); 老年模型组较老年正常组穿台次数明显减少(P<0.05),老年中药组及老年模型组均较老年正常组在第Ⅲ象限路程及第Ⅲ象限时间明显缩短(P<0.05)。 结论 老年中药组及老年正常组小鼠Morris水迷宫成绩较理想,说明补肾祛瘀化痰法可改善老龄小鼠Morris水迷宫成绩,从而改善认知功能。
Abstract:
Objective To assess the effects of tonifying kidney and removing stasis and phlegm on the test of morris water maze of ApoE knockout mice in adult and old groups. Methods ApoE knockout mice were divided into adult treatment group, adult model group, old treatment group and old model group. And C57BL/6J mice were divided into adult normal groups and old normal groups. When the adult groups mice were 32 weeks and the old groups were 44 weeks, the mice were tested by morris water maze. The scores of navigation experiment and space exploration experiment in morris water maze were recorded. Results The escape latency of adult rats in the treatment group, model group and normal group had no significantly difference(P>0.05). But in old rats, the escape latency of the model group was significantly longer than that of the normal group and treatment group. The space exploration experiment showed that the normal group was much better than the treatment and model groups. Conclusions The normal group and the treatment group show better scores in morris water maze than the model group. The results support that tonifying kidney and removing stasis and phlegm can improve cognitive function of old ApoE knockout mice.

参考文献/References:

[1] Masliah E, SamuelW, Veinbergs I, et al. Neurodegeneration and cognitive impairment in ApoE-deficient mice is ameliorated by infusion of recombinant ApoE[J]. Brain Res, 1997, 751(12):307-314.
[2] Hafezi-Moghadam A, Thom as KL, Wagner DD. ApoE deficiency leads to a progressive age-dependent blood-brain barrier leakage[J]. Am J Physiol Cell Physiol,2007, 292(4):1256-1262.
[3] Zhang N, Jia XM, Xu L, et al. The expression change of amyloid protein β in the hippocampal neurons of ApoE knockoutmice[J]. Acta Universitatis Medicinalis Anhui, 2011, 46(6):531-534.
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备注/Memo

备注/Memo:
基金项目: 北京中医药大学博士自主课题(2013-JYB22-XS-128)
作者单位: 100035 北京积水潭医院中医科(姜洋、马玉棋、梁志齐); 北京中医药大学第一临床医学院教务处(杨惠民)
作者简介: 姜洋(1986- ),女,博士,住院医师。研究方向:中医老年病学。E-mail:jiangyang310@sohu.com
更新日期/Last Update: 2016-01-06