|本期目录/Table of Contents|

[1]宿世震,项东宇,孟繁伟,等.翻白草黄酮对2型糖尿病大鼠胰岛细胞的保护作用及机制研究[J].环球中医药,2016,9(02):145-148.[doi:10.3969/j.issn.1674-1749.2016.02.005]
　XU Shi-zhen,XIANG Dong-yu,MENG Fan-wei,et al.Flavonoids from Potentilla discolor Bunge playing a protective and mechanical role for the type 2 diabetic rats islet cell[J].,2016,9(02):145-148.[doi:10.3969/j.issn.1674-1749.2016.02.005]
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翻白草黄酮对2型糖尿病大鼠胰岛细胞的 保护作用及机制研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年02期
页码:
145-148
栏目:
论著
出版日期:
2016-02-06

文章信息/Info

Title:
Flavonoids from Potentilla discolor Bunge playing a protective and mechanical role for the type 2 diabetic rats islet cell
作者:
宿世震；项东宇；孟繁伟；张钦德
264199 烟台,山东中医药高等专科学校西医教学部[宿世震(硕士研究生)、孟繁伟],中药系(项东宇、张钦德)
Author(s):
XU Shi-zhen;  XIANG Dong-yu;  MENG Fan-wei;  et al.
Shandong college of traditional Chinese medicine, Yantai 264199, China
关键词:
翻白草;  黄酮;  2型糖尿病;  大鼠;  保护作用
Keywords:
Potentilla discolor;  Flavonoids;  Type 2 diabetes;  Rat;  Protective effect
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.02.005
文献标志码:
A
摘要:
目的 研究翻白草黄酮对2型糖尿病大鼠胰岛细胞的保护作用及机制。方法 采用高脂高糖饲料喂养大鼠6周后,腹腔注射链脲佐菌素复制2型糖尿病大鼠模型。成模大鼠随机分为模型组、阳性药物对照组(罗格列酮,4 mg/kg)、翻白草黄酮低剂量组(3 g/kg)、翻白草黄酮高剂量组(6 g/kg),同批次健康大鼠作为实验的正常对照组,每组10只。药物干预4周后,微量血糖仪检测空腹血糖(fasting blood glucose,FBG)、放免法测定空腹胰岛素(fasting insulin,FINS)并计算胰岛素敏感性指数(insulin sensitivity index,ISI)、ELISA法测定肿瘤坏死因子(tumor necrosis factor-α,TNF-α)及白细胞介素-6(interleukin-6,IL-6)水平; 部分胰腺组织匀浆后黄嘌呤氧化酶法测定超氧化物歧化酶(superoxide dismutase,SOD)水平、硫代巴比妥酸法测定丙二醛(malondialdehyde,MDA)含量; 取部分胰腺制作病理切片,光镜下观察胰腺组织形态变化。结果 翻白草黄酮可降低大鼠的FBG、FINS、TNF-α、IL-6水平及改善胰岛素抵抗,明显提高大鼠胰腺组织中的SOD水平、降低MDA含量。病理切片显示胰岛细胞损伤明显减轻,翻白草黄酮高剂量组(6.0 g/kg)作用明显。结论 翻白草黄酮对2型糖尿病大鼠胰岛细胞具有保护作用,其保护作用可能是通过减轻胰腺组织内的氧化应激水平及降低全身炎症反应而实现的。
Abstract:
Objective This article aims to study the protective mechanism of Flavonoids from Potentilla Discolor Bunge(PDB)to the type 2 Diabetic rats islet cell. Methods Using high fat-high sucrose diet to feed rats for 6 weeks, then the researchers copy the type 2 diabetic rats model with Streptozotocin(STZ). Model rats are randomly divided into the model group, the positive drug control group(rosiglitazone hydrochloride, 4 mg/kg), the low dose of flavone group(3 g/kg), and the high dose of flavone group(6 g/kg). At the same time, healthy rats are used as the experimental rats of normal group with 10 rats in each group. They are intervened by drug for 4 weeks. Then, trace blood sugar appearance tests fasting blood-glucose, radio immunity measures fasting insulin(FINS)and calculates insulin sensitivity index(ISI)and ELISA measures the level of tumor necrosis factor(TNF-α)and Interleukin-6(IL-6). Meanwhile, part of the pancreas tissue homogenate after xanthine oxidase method measures the level of SOD, and TBA measures the content of malondialdehyde(MDA). Take part of the pancreas pathological slices, and then observe the shape variation of the pancreas. Results Flavone from Potentilla Discolor Bunge(PDB)can reduce FBG, FINS, TNF alpha, and IL-6 levels and can improve insulin resistance. In addition, it is clear that it improves the level of SOD in the pancreatic tissue of rats and decreases MDA content. Pathological section shows an apparent reduction of the damage to islet cell, which is obvious in the high dose of flavonoids(6 g/kg). Conclusion Flavonoids from Potentilla Discolor Bunge(PDB)plays a protective role for type 2 Diabetic rats islet cell. In addition, the mechanism of its protective effect may through reducing the oxidative stress level in the pancreatic tissue and reducing the systemic inflammatory response.

参考文献/References:

[1] Piro S,Rabuazzo AM,Renis M,et al. Effects of Metformin on oxidative stress,adenine nucleotides balance and glucose-induced insulin release impaired by chronic free fatty acids exposure in rat pancreatic islets[J].J Endocrinol Invest,2012,35(5):504-510.
[2] 洪旭,陈海平,于恒池.老年2型糖尿病患者血清炎症因子水平及其与大血管病变的相关性[J].中国糖尿病杂志,2011,19(8):571-574.
[3] 刘顺,单淇,周渭渭,等.翻白草糖尿病防治物质基础研究近况与展望[J].世界科学技术-中医药现代化,2010,12(2):165-171.
[4] 曹兰秀,张振宇.翻白草对胰岛素抵抗大鼠的影响[J].辽宁中医药大学学报,2011,13(8):196-197.
[5] 尹长江,杨坤宝,张学军,等.枸杞多糖对2型糖尿病大鼠的降糖作用研究[J].中成药,2014,36(8):1750-1753.
[6] Brownlee M. The pathobiology of diabetic complications:a unifying mechanism [J].Diabetes,2005,54(6):1615-1625.
[7] Rains JL,Jain SK. Oxidative stress,insulin signaling, and diabetes[J]. Free Radic Biol Med,2011,50(5):567-575.
[8] Kim MJ,Jung KH,Uhm YK,et al. Preservative effect of electrolyzed reduced water on pancreatic beta-cell mass in diabetic db/db mice[J].Biol Pharm Bull,2007,30(2):234-236.
[9] Arora P,Garcia-Bailo B,Dastani Z,et al.Genetic polymorphisms of innate immunity-related inflammatory pathways and their association with factors related to type 2 diabetes[J].BMC Med Genet,2011,12:95-99.
[10] 崔思远,李芳.IL-6与2型糖尿病关系的研究进展[J].中国微生态杂志,2012,24(11):1051-1054.
[11] Bruun JM,Roeske-Nielsen A,Richelsen B,et al. Sulfatide increases adiponectin and decreases TNF-alpha,IL-6,and IL-8 in human adipose tissue in vitro[J]. Mol Cell Endocrinol,2007,263(1-2):142-148.
[12] Matthews VB,Allen TL,Risis S,et al. Interleukin-6-deficient mice develop hepatic inflammation and systemic insulin resistance[J].Diabetologia,2010,53(11):2431-2441.

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备注/Memo

备注/Memo:
作者单位: 264199 烟台,山东中医药高等专科学校西医教学部[宿世震(硕士研究生)、孟繁伟],中药系(项东宇、张钦德)
作者简介: 宿世震(1979- ),2015级在读硕士研究生,讲师。研究方向:动物模型及解剖学研究。E-mail:ssz169@126.com
更新日期/Last Update: 2016-02-06