|本期目录/Table of Contents|

[1]张建美,许皖,钟赣生,等.甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠水通道蛋白-2基因及肾凋亡的影响[J].环球中医药,2016,9(05):539-543.[doi:10.3969/j.issn.1674-1749.2016.05.005]
　ZHANG Jian-mei,XU Wan,ZHONG Gan-sheng,et al.Comparing the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza on AQP-2 mRNA expression and apoptosis rate of kidney tissue in ascites rats under different times[J].,2016,9(05):539-543.[doi:10.3969/j.issn.1674-1749.2016.05.005]
点击复制

甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠水通道蛋白-2基因及肾凋亡的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年05期
页码:
539-543
栏目:
甘遂甘草反药研究专题
出版日期:
2016-05-06

文章信息/Info

Title:
Comparing the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza on AQP-2 mRNA expression and apoptosis rate of kidney tissue in ascites rats under different times
作者:
张建美; 许皖; 钟赣生; 赵桐; 范盎然; 柳海艳; 郭岩松; 修琳琳; 王思睿
100029 北京中医药大学基础医学院[张建美(硕士研究生)、许皖(硕士研究生)、郭岩松(硕士研究生)、修琳琳(博士研究生)、王思睿(硕士研究生)],基础医学院方药系(钟赣生、柳海艳),科研实验中心(范盎然); 中国中医药出版社(赵桐)
Author(s):
ZHANG Jian-mei;  XU Wan;  ZHONG Gan-sheng;  et al.
School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
甘遂;  甘草;  反药组合;  水通道-2信使RNA;  肾组织凋亡
Keywords:
Kansui;  Glycyrrhiza;  Anti herb combination;  Aquaporin-2 mRNA;  Apoptosis of kidney tissue
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.05.005
文献标志码:
A
摘要:
目的 观察不同给药次数条件下的甘遂半夏汤加减甘遂甘草反药组合对腹水模型大鼠肾脏水通道蛋白(aquaporin, AQP)-2 mRNA 表达及肾组织凋亡的影响。 方法 采用Walker-256细胞制造癌性腹水模型,将Wistar大鼠按体质量随机分为空白组、模型组、阳性对照组、全方给药一次组、去草给药一次组、去遂给药一次组、去草遂给药一次组、全方给药二次组、去草给药二次组、去遂给药二次组、去草遂给药二次组,共计11组。给药一次组每天上午给药一次,给药二次组上午下午各给药一次,一二次组每天给药总量一致。腹水长成14天取材,摘取肾脏,液氮保存,进行PCR和免疫组化实验。 结果 甘遂半夏汤全方给药一次组和全方给药二次组的AQP-2 mRNA 表达及肾组织凋亡无明显差异。但全方组的AQP-2 mRNA表达及肾组织凋亡均较全方去一味或两味反药组有降低趋势。 结论 含甘遂甘草反药组合的甘遂半夏汤全方给药一次组和给药二次组抑制AQP-2 mRNA表达和对癌性腹水造成的肾组织细胞过度凋亡的减缓作用无明显差异,且均优于全方去掉一味或两味反药组。
Abstract:
Objective To find out the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza under different times of administration on AQP-2 mRNA expression and apoptosis rate of kidney tissue in malignant ascites rats. Methods Walker-256 cells were used to induce the malignant ascites rat model. The Wistar rats were randomly divided into blank group, model group, furosemide group, once or twice of Gansui Banxia decoction group, once or twice of Gansui Banxia decoction removed glycyrrhiza group, once or twice of Gansui Banxia decoction removed kansui group, once or twice of Gansui Banxia decoction removed glycyrrhiza and kansui group. Once group was gavaged at morning, and twice group was gavaged at morning and afternoon, which total dose was as much as the once group. After 14 days' feed, kidney was collected and kep in liquid nitrogen and then RT-PCR and immunohistochemistry experiments was conducted. Results The result of AQP-2 mRNA expression and apoptosis rate of kidney tissue showed no difference in once and twice Gansui Banxia decoction group, but showed a decreasing tendency compared to the groups removed one or two imcompatible pairs. Conclusion Diuresis of Gansui Banxia decoction is better than it removed one or two imcompatible pairs. Gansui Banxia decoction can reduce the excessive apoptosis of kidney tissue and the effect of whole prescriptionis is better than it removed one or two imcompatible pairs.

参考文献/References:

[1] 范永升.金匮要略[M].北京:中国中医药出版社,2007:160.
[2] 赵桐,钟赣生,张建美,等.含甘遂-甘草反药组合的甘遂半夏汤临床用药分析[J].中国临床医生杂志,2014,(12):89-91.
[3] 张建美,许皖,钟赣生,等.甘遂甘草反药组合宜忌条件的实验研究回顾与评价[J].环球中医药,2015,8(9):25-30.
[4] 张建美,许皖,钟赣生,等.大戟甘草反药组合宜忌条件的实验研究回顾与评价[J].环球中医药,2015,8(9):38-42.
[5] 唐庆娟.水通道蛋白家族成员AQP2的研究进展[J].国外医学(生理、病理科学与临床分册),2001,21(3):238-240.
[6] 刘彦华,才丽平.肾脏AQP2表达及穿梭调节的分子机制[J].国外医学(生理、病理科学与临床分册),2004,24(5):463-466.
[7] 王建华.肝硬化腹水患者血浆AVP和尿AQP2的检测及临床意义[J].临床合理用药杂志,2013,6(17):155.
[8] 李超,伏圣博,刘华玲,等.细胞凋亡研究进展[J].世界科技研究与发展,2007,29(3):45-53.

相似文献/References:

[1]张建美,许皖,钟赣生,等.甘遂甘草反药组合宜忌条件的实验研究回顾与评析[J].环球中医药,2015,8(09):1053.[doi:10.3969/jissn.1674-1749.2015.09.006]
　ZHANG Jian mei,XU Wan,ZHONG Gan sheng,et al.Review on the experimental studies of suitable and contraindicant conditions of Chinese medicine antagonism compatibility kansui radix and glycyrrhiza[J].,2015,8(05):1053.[doi:10.3969/jissn.1674-1749.2015.09.006]
[2]郭岩松,柳海艳,钟赣生,等.甘遂半夏汤加减甘遂及不同品种甘草对腹水模型大鼠心肝肾功能的影响[J].环球中医药,2016,9(05):527.[doi:10.3969/j.issn.1674-1749.2016.05.003]
　GUO Yan-song,LIU Hai-yan,ZHONG Gan-sheng,et al.Comparing the effect of Gansui Banxia decoction plus or reduce kansui and varieties of glycyrrhiza on heart, liver and kidney function of ascites model rat[J].,2016,9(05):527.[doi:10.3969/j.issn.1674-1749.2016.05.003]
[3]张建美,许皖,钟赣生,等.甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠肝脏病理形态的影响[J].环球中医药,2016,9(05):534.[doi:10.3969/j.issn.1674-1749.2016.05.004]
　ZHANG Jian-mei,XU Wan,ZHONG Gan-sheng,et al.Comparing the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza on pathological morphology of liver of ascites rat under different times[J].,2016,9(05):534.[doi:10.3969/j.issn.1674-1749.2016.05.004]
[4]王思睿 修琳琳 刘殿娜 陈丰 柳海艳 陈绍红 李娜 张晨 高洁 钟赣生.含不同品种海藻与甘草反药组合的海藻玉壶汤对甲状腺肿大大鼠模型的影响[J].环球中医药,2017,10(06):541.[doi:10.3969/j.issn.1674-1749.2017.06.001]
　WANG Sirui,XIU Linlin,LIU Dianna,et al.Effect of incompatible herb of different varieties of seaweed combination with glycyrrhiza of Haizao Yuhu decoction on thyromegaly rats model[J].,2017,10(05):541.[doi:10.3969/j.issn.1674-1749.2017.06.001]

备注/Memo

备注/Memo:
基金项目: 国家重点基础研究发展计划(973计划)(2011CB505306); 北京中医药大学2015年度基本科研业务费项目(在读研究生项目)(2015-JYB-XS033)
作者简介: 张建美(1986- ),女,2013级在读硕士研究生。研究方向:中药药性理论研究。E-mail:kkmm20110814@163.com
通讯作者: 钟赣生(1961- ),硕士,教授,博士生导师。研究方向:中药药性理论研究。 E-mail:zhonggansheng@sohu.com
更新日期/Last Update: 2016-05-06