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[1]王思颖,李绍旦,刘毅,等.通心络对心肌梗死模型大鼠心肌损伤保护作用及机制的研究[J].环球中医药,2016,9(06):650-653.[doi:10.3969/j.issn.1674-1749.2016.06.002]
　WANG Si-ying,LI Shao-dan,LIU Yi,et al.Protective effect and mechanism of Tongxinluo on myocardial injury in myocardial infarction model rats[J].,2016,9(06):650-653.[doi:10.3969/j.issn.1674-1749.2016.06.002]
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通心络对心肌梗死模型大鼠心肌损伤保护作用及机制的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年06期
页码:
650-653
栏目:
论著
出版日期:
2016-06-06

文章信息/Info

Title:
Protective effect and mechanism of Tongxinluo on myocardial injury in myocardial infarction model rats
作者:
王思颖; 李绍旦; 刘毅; 冯宇; 张俊修; 霍旺; 高路; 杨明会
100700 北京中医药大学东直门医院[王思颖(博士研究生)]; 中国人民解放军总医院中医科[王思颖、李绍旦、刘毅、冯宇、张俊修、霍旺、高路、杨明会]
Author(s):
WANG Si-ying; LI Shao-dan; LIU Yi; et al.
Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700,China
关键词:
通心络;  心肌梗死;  模型大鼠;  凋亡;  氧化应激;  内皮功能
Keywords:
Tongxinluo;  Myocadial infarction;  Model rat;  Apoptosis;  Oxidative stress;  Endothelial function
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.06.002
文献标志码:
A
摘要:
目的 探索通心络对心肌梗死模型大鼠心肌损伤的保护作用及机制。方法 采用结扎前降支方法制作30只心肌梗死大鼠模型,随机分为模型组、通心络组、培哚普利组,每组10只; 假手术组9只仅穿线不结扎。药物组大鼠分别以通心络、培哚普利灌胃,模型组和假手术组给予等量羧甲基纤维素钠溶液灌胃。2周后测量TUNEL凋亡指数,以及血清超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、一氧化氮(nitrogen monoxide,NO)、内皮素-1(endothelin-1,ET-1)水平。结果 药物组大鼠的一般情况均有所改善。通心络组、培哚普利组的凋亡指数和MDA、ET-1水平较模型组均明显降低,SOD、NO水平较模型组均明显升高(P<0.05)。结论 通心络可抑制心肌细胞凋亡,并通过升高SOD、NO水平,降低MDA、ET-1水平,改善氧化应激状态和内皮功能,保护心肌组织。
Abstract:
Objective To study the protective effect and mechanism of Tongxinluo on myocardial injury in myocardial infarction model rats. Methods The rat model of acute myocardial infarction was established by ligaturing the left anterior descending coronary artery. 30 model rats were randomly divided into the model group, Tongxinluo group, Perindopril group, 10 rats in each group. And 9 rats in sham group were punctured only without ligation. The rats in each medication group were intragastric administration respectively by Tongxinluo suspension and Perindopril suspension with corresponding dosage. And the model group and the sham group were intragastric administration by sodium carboxymethyl cellulose solution with corresponding dosage. All the rats were given continuously for 2 weeks. The apoptotic index and the level of SOD, MDA, NO and ET-1 were measured by corresponding methods. Results The general condition of rats in medication groups was better than model group. The level of SOD and NO in Tongxinluo group and Perindopril group was increased more significantly than that of the model group(P<0.01). The level of MDA and ET-1 in Tongxinluo group and Perindopril group was decreased more significantly than model group(P<0.05). Conclusions Tongxinluo can inhibit myocardial apoptosis, and protect the cardiac muscle tissue by increasing the level of SOD and NO, decreasing the level of MDA and ET-1, and it also can improve the oxidative stress and perform protective effect on myocardial tissue endothelial function.

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备注/Memo

备注/Memo:
基金项目: 国家重点基础研究发展计划(973计划)(2012CB518601)
作者简介: 王思颖(1986- ),女,2013级在读博士研究生。研究方向:中西医结合治疗老年病。E-mail:lucky18603@163.com
通讯作者: 杨明会(1962- ),博士,教授,主任医师,博士生导师。研究方向:中西医结合基础与临床。E-mail:ymh9651@sina.com
更新日期/Last Update: 2016-06-06