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[1]杨丹,梁晓春,屈岭,等.中药筋脉通对糖尿病大鼠背根神经节神经元超微结构影响的研究[J].环球中医药,2016,9(06):654-658.[doi:10.3969/j.issn.1674-1749.2016.06.003]
　YANG Dan,LIANG Xiao-chun,QU Ling,et al.Effects of Jinmaitong on ultrastructure of DRG neurons in STZ-DM rats[J].,2016,9(06):654-658.[doi:10.3969/j.issn.1674-1749.2016.06.003]
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中药筋脉通对糖尿病大鼠背根神经节神经元超微结构影响的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年06期
页码:
654-658
栏目:
论著
出版日期:
2016-06-06

文章信息/Info

Title:
Effects of Jinmaitong on ultrastructure of DRG neurons in STZ-DM rats
作者:
杨丹; 梁晓春; 屈岭; 吴群励; 吴亚楠; 石玥; 戴威
100730 中国医学科学院 北京协和医学院 北京协和医院中医科转化医学中心(杨丹、梁晓春、屈岭、吴群励、吴亚楠、石玥); 中国医学科学院基础医学研究所电镜室(戴威)
Author(s):
YANG Dan;  LIANG Xiao-chun;  QU Ling;  et al.
Department of Traditional Chinese Medicine, PUMC Hospital, PUMC&CAMS, Beijing 100730, China
关键词:
糖尿病周围神经病变;  背根神经节神经元;  筋脉通;  超微结构;  线粒体
Keywords:
Diabetic peripheral neuropathy;  Dorsal root ganglia;  Jinmaitong;  Ultrastructure;  Mitochondria
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.06.003
文献标志码:
A
摘要:
目的 观察中药筋脉通对链尿佐菌素(streptozotocin,STZ)诱导的糖尿病(diabetes mellitus,DM)大鼠背根神经节(dorsal root ganglia,DRG)感觉神经元超微结构的影响。方法 雄性SD大鼠18只,腹腔内注射STZ诱导建立糖尿病大鼠模型,随机分为糖尿病组、筋脉通组与牛磺酸组,每组6只大鼠,并设正常对照组6只。成模后每天1次灌胃给药,持续16周。药物干预16周后,用电子Von Frey仪检测机械痛阈值,取大鼠背根神经节,按常规方法制成切片后,在电镜下观察背根神经节神经元超微结构的病理学改变。结果 糖尿病大鼠的机械痛阈值较正常组显著明显下降(P<0.01),背根神经节感觉神经元超微结构出现重度损伤,神经元细胞中线粒体数量显著减少(P<0.01),并且线粒体肿胀及空泡化比率、凋亡率显著升高(P<0.01); 中药筋脉通可显著提高机械痛阈值(P<0.01),减轻神经元的损伤,提高神经元中线粒体的数量(P<0.05),并且降低线粒体的肿胀及空泡化比率及凋亡率(P<0.01),且中药筋脉通的疗效优于牛磺酸(P<0.01)。结论 中药筋脉通对抑制糖尿病大鼠背根神经节感觉神经元及其线粒体超微结构的损伤而对糖尿病所致周围神经病变起到一定的保护作用。
Abstract:
Objective To study the effects of Jinmaitong on ultrastructure of dorsal root ganglion(DRG)neurons in STZ-induced diabetic rats.Methods The STZ-induced diabetic rats were randomly divided into 4 groups including normal control group(Con), Diabetic group(DM), JMT group(JMT)and Taurine group(Tau). Six rats were in each group. All rats were given intragastric administration for 16 weeks and then culled.The mechanical withdrawal threshold was tested by electronic Von Frey instrument before death.The DRG of the rats was collected and used for transmission electron microscopy. Results Compared with the control group, the mechanical pain threshold of the DM group was significantly decreased(P<0.01). The ultrastructure of mitochondria in DRG neurons of the DM group was severely damaged. The number of mitochondria in neurons was significantly decreased(P<0.01), and the rate of swelling mitochondria and mitochondria vacuolar degeneration in DRG neurons was significantly increased of the DM group(P<0.01). The mechanical pain threshold and the number of mitochondria of two treatment groups were significantly improved compared with the DM group.The rate of swelling mitochondria and mitochondria vacuolar degeneration of two treatment groups were decreased strikingly(P<0.01), and the JMT group was more effective than the Tau group(P<0.01). Conclusion JMT could ameliorate the peripheral neuropathy by improving the pathological morphology of mitochondria in DRG neurons.

参考文献/References:

[1] Callaghan BC,Cheng HT,Stables CL,et al. Diabetic neuropathy:clinical manifestations and current treatments[J]. Lancet Neurol,2012,11(6):521-534.
[2] 梁晓春,崔丽英,郭赛珊,等.筋脉通治疗糖尿病周围神经病变的临床观察[J].中国中西医结合杂志,1999,19(9):517-519.
[3] Yagihashi S,Yamagishi S,Wada R. Pathology and pathogenetic mechanisms of diabetic neuropathy:correlation with clinical signs and symptoms[J]. Diabetes Res Clin Pract,2007,77(Suppl 1):S184-S189.
[4] Calcutt NA,Jolivalt CG,Fernyhough P. Growth factors as therapeutics for diabetic neuropathy[J]. Curr Drug Targets,2008,9(9):47-59.
[5] Tomlinson DR,Gardiner NJ. Glucose neurotoxicity[J]. Nat Rev Neurosci,2008,9(1):36-45.
[6] Brownlee M. Biochemistry and molecular cell biology of diabeticcomplications[J]. Nature,2001,414(6865):813-820.
[7] FernyhoughP,Huang TJ,Verkhratsky A. Mechanism of mitochondrial dysfunction in diabetic sensory neuropathy[J].J Peripher Nerv Syst,2003,8(4):227-235.
[8] Verkhratsky A and Fernyhough P. Mitochondrial malfunction and Ca2+ dyshomeostasis drive neuronal pathology in diabetes[J].Cell Calcium,2008,44(1):112-122.
[9] Kang D, Hamasaki N. Alterations of mitochondrial DNA in common diseases and disease states:aging,neurodegeneration,heart failure,diabetes,and cancer[J]. Curr Med Chem,2005,12(4):429-441.
[10] ZeevalkGD,Bernard LP,Song C,et al. Mitochondrial inhibition and oxidative stress:reciprocating players in neurodegeneration[J]. Antioxid Redox Signal,2005,7(9-10):1117-1139.
[11] Beal MF.Mitochondria take center stage in aging and neurodegeneration[J]. Ann Neurol,2005,58(4):495-505.
[12] VincentAM,FelanEL. New insights into the mechanisms of diabetic neuropathy[J]. Rev Endocr Metab Disord,2004,5(3):227-236.
[13] Vincent AM,Edwards JL,McLean LL,et al. Mitochondrial biogenesis and fission in axons in cell culture and animal models of diabetic neuropathy[J]. Acta Neuropathol,2010,120(4):477-489.

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备注/Memo

备注/Memo:
基金项目: 北京市自然科学基金(7122147)
作者简介: 杨丹(1980- ),女,博士,主治医师。研究方向:糖尿病慢性并发症的中西医结合临床研究。E-mail:jsj000jsj@163.com
通讯作者: 梁晓春(1956- ),女,博士,教授,博士生导师。中国中西医结合学会常务理事,中国中西医结合学会内分泌代谢病专业委员会副主任委员,北京中西医结合学会糖尿病专业委员会主任委员。研究方向:中西医结合防治糖尿病慢性并发症。E-mail: xcliang@vip.sina.com
更新日期/Last Update: 2016-06-06