|本期目录/Table of Contents|

[1]方锦颖 陈琪 国文文 杨德爽 孟州令 陈光耀 吴卓耘 王素梅.龟板对多发性抽动症模型大鼠的干预作用与对DA及D2受体的影响[J].环球中医药,2016,9(07):781-784.[doi:10.3969/j.issn.1674-1749.2016.07.002]
　FANG Jin-ying,CHEN Qi,GUO Wen-wen,et al.Intervention effect of Plastrum Testudinis on rat model of TS and DA and D2 receptor[J].,2016,9(07):781-784.[doi:10.3969/j.issn.1674-1749.2016.07.002]
点击复制

龟板对多发性抽动症模型大鼠的干预作用与对DA及D2受体的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年07期
页码:
781-784
栏目:
论著
出版日期:
2016-07-06

文章信息/Info

Title:
Intervention effect of Plastrum Testudinis on rat model of TS and DA and D2 receptor
作者:
方锦颖 陈琪 国文文 杨德爽 孟州令 陈光耀 吴卓耘 王素梅
100029 北京中医药大学基础医学院[方锦颖、陈琪、国文文、杨德爽、孟州令、陈光耀、吴卓耘(硕士研究生)]；北京中医药大学东方医院儿科(王素梅)
Author(s):
FANG Jin-ying; CHEN Qi; GUO Wen-wen; et al.
Shool of Basic Medical Sciences,Beijing University of Traditional Chinese Medicine, Beijing 100029, China
关键词:
多发性抽动症; 龟板; 多巴胺; 多巴胺D2受体
Keywords:
Tourette syndrome; Plastrum Testudinis; Dopamine; Dopamine D2 receptor
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.07.002
文献标志码:
A
摘要:
目的 探究龟板对多发性抽动症(tourette syndrome,TS)模型大鼠的行为学改变,及其对纹状体多巴胺(dopamine,DA)、多巴胺D2受体(dopamine D2 receptor,D2R)、血浆中DA的含量的影响。 方法 将40只大鼠分为空白组、模型组、硫必利组和龟板组。腹腔注射亚氨基二丙腈(iminodipropionitrile,IDPN)建立TS模型,并给予每组相应的生理盐水或药物灌胃。造模完成后每周进行行为学检测,并于造模第4周采用ELISA法检测纹状体DA、D2R、血浆中DA的含量。结果(1)与模型组比较, 两周后硫必利组大鼠刻板行为减少(P＜0.05),三周后运动行为减少(P＜0.05),四周后运动行为明显减少(P＜0.01)。与模型组比较,龟板组两周后大鼠刻板行为与运动行为均减少(P＜0.05);(2)模型组大鼠较空白组大鼠血浆中DA含量升高(P＜0.05),纹状体DA含量降低(P＜0.05),纹状体D2R含量升高(P＜0.05)。与模型组比较,龟板组大鼠血浆DA含量减少(P＜0.05),硫必利组血浆DA明显减少(P＜0.01),纹状体DA含量两组均升高(P＜0.05),两组纹状体D2R未发生明显变化(P＞0.05)。结论 龟板对TS有良好的治疗作用,其原因可能与调节DA的代谢有关。
Abstract:
Objective To investigate the behavior change of Plastrum Testudinis on Tourette's syndrome(TS)in a rat model, and the influence of Plastrum Testudinis on striatal dopamine(DA), dopamine D2 receptor(D2R)and the content of DA in plasma, and provide neural biochemical basis of Plastrum Testudinis in treatment of TS. Methods 40 rats were divided into blank group, model group, Tiapride group and Plastrum Testudinis group. Rats were injected intraperitoneally with iminodipropionitrile(IDPN)to establish the TS model, and the rats weregiven corresponding normal saline and drugs. Behavioral testing was performed weekly after the model was completed, and the contents of DA, D2R and DA in the striatum were detected by ELISA method at fourth weeks after the model was made. Results(1)Compared with model group, stereotyped behavior of Tiapride group was reduced(P＜0.05)after 2weeks, motor behavior was reduced after 3weeks(P＜0.05), motor behavior was reduced obviously after 4weeks(P＜0.01). Compared with model group, stereotyped behavior and motor behavior of Plastrum Testudinis group was reduced(P＜0.05).(2)Compared with blank group, DA in plasma of model group was increased(P＜0.05); DA in corpus striatum was reduced(P＜0.05)and D2R in corpus striatum was increased(P＜0.05). Compared with model group, DA in plasma was reduced in Plastrum Testudinis group(P＜0.05)and significantly reduced in Tiapride group(P＜0.01), DA in corpus striatum were all increased in two group(P＜0.05), D2R in corpus striatum did not change obviously(P＞0.05). Conclusions The influence of Plastrum Testudinis on rat model of TS was significantly, and the reason maybe related to the regulation of DA metabolism.

参考文献/References:

[1] 李雄.儿童多发性抽动症的研究进展[J].医学综述,2012,18(9):1352-1354.
[2] 王道涵. 健脾止动汤对TS小鼠纹状体多巴胺系统DR、DAT及谷氨酸系统GLT1的影响[D].北京:北京中医药大学,2013.
[3] 王道涵,王素梅,张雯,等. IDPN诱导TS模型小鼠纹状体EAAT2表达变化及健脾止动汤的干预研究[J]. 中华中医药杂志,2013,28(5):1471-1474.
[4] 郝宏文,王素梅.王素梅扶土抑木法治疗多发性抽动症经验[J].中国中医药信息杂志,2010,17(3):86-87.
[5] 平静,黄伟.中药治疗儿童多发性抽动症现代文献分析[J].中国中西医结合儿科学,2011,3(5):393-395.
[6] 邓汝东,李伊为,陈东风,等.龟板抗Parkinson病大鼠多巴胺能神经元凋亡的作用[J].神经解剖学杂志,2008,24(3):301-306.
[7] 李伊为,周健洪,陈东风,等.龟板对帕金森病大鼠行为和脑内多巴胺水平的影响[J].解剖学研究,2004,26(1):17-18,21.
[8] Al Kadasah S,Al Mutairy A,Siddiquei M,et al. Pentoxifylline attenuates iminodipropionitrile-induced behavioral abnormalities in rates[J].Behav Pharmacol,2009,20(4); 356-360.
[9] Diamond BI,Reyes MG,Borison R.A new animal model for Tourette syndromes[J].Advances in Neurology,1982,35:221-225.
[10] 卫利,王素梅,崔霞,等.多发性抽动症动物模型的比较与评价[J].中华行为医学与脑科学杂志,2012,21(2):187-189.
[11] 卫利,王素梅,陈自佳,等.化学因素诱发的多发性抽动症动物模型的比较研究[J].中华行为医学与脑科学杂志,2010,19(8):683-686.
[12] Bronfeld M,Israelashvili M, Bar-Gad I.Pharmacological animal models of Tourette syndrome[J]. Neurosci Biobehav Rev,2012,7634(12): 163-167.
[13] 李红梅. 硫必利治疗小儿多发性抽动症的临床研究[J]. 中国医学工程,2013,21(11):172-173.
[14] 解莹晶,朱先康.小儿多发性抽动症的相关实验研究进展[J].成都中医药大学学报,2014,37(1):121-124.
[15] 朱舜丽,唐久来,陈全珠,等. 儿童多发性抽动症的神经生化学机制探讨[J]. 临沂医专学报,1997,19(4):253-255.

相似文献/References:

备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81273799)；国家级大学生创新创业训练计划(201510026011)；北京市大学生科学研究与创业行动计划专项(BJGJ1616)
作者简介: 方锦颖(1995- ),女,2014级本硕连读本科阶段在读。研究方向:神经精神疾病病证结合研究。E-mail:fangjinying1@163.com
通讯作者: 王素梅(1950- ),女,博士,教授,博士生导师。研究方向:小儿肝系疾病。E-mail:wangsumei120@163.com
更新日期/Last Update: 2016-07-06