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[1]张永成 姜银松 王占青.大黄联合乌司他丁对百草枯中毒大鼠肺损伤保护作用[J].环球中医药,2016,9(07):795-797.[doi:10.3969/j.issn.1674-1749.2016.07.005]
　Zhang Yong-cheng,Jiang Yin-song,Wang Zhan-qing..The protection effect of Rhubarb combine with Ulinastatin on paraquat poisoning induced lung injury in rats[J].,2016,9(07):795-797.[doi:10.3969/j.issn.1674-1749.2016.07.005]
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大黄联合乌司他丁对百草枯中毒大鼠肺损伤保护作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年07期
页码:
795-797
栏目:
论著
出版日期:
2016-07-06

文章信息/Info

Title:
The protection effect of Rhubarb combine with Ulinastatin on paraquat poisoning induced lung injury in rats
作者:
张永成 姜银松 王占青
264100 滨州医学院烟台附属医院急诊科
Author(s):
Zhang Yong-cheng;  Jiang Yin-song;  Wang Zhan-qing.
Department of Emergency, the Affiliated Hospital of Bin Zhou Medical College, Yantai 264100, China
关键词:
百草枯; 大黄; 乌司他丁; 肿瘤坏死因子-α; 转化生长因子-β1
Keywords:
Paraquat; Rhubarb; Ulinastatin; TNF-α; TGF-β1
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.07.005
文献标志码:
A
摘要:
目的 观察大黄联合乌司他丁对急性百草枯中毒大鼠肺组织病理变化以及血浆中TNF- α、TGF-β1含量的干预作用。 方法 将72只SD大鼠随机分为对照组8只、染毒组32只、干预组32只。染毒组、干预组分别于第1天、3天、5天、7天分批处死存活大鼠,光镜下观察肺组织变化,并测定血浆TNF- α、TGF-β1的含量。 结果 染毒组大鼠肺组织出现明显的出血、炎症细胞浸润、肺实变等变化; 干预组较染毒组病理损伤减轻; 染毒组、干预组TNF-α、TGF-β1水平在染毒后1天开始升高,与对照组相比,差异有统计学意义(P＜0.01),在相同时间点干预组血浆TGF-β1、TNF- α表达低于染毒组,差异均有统计学意义(P＜0.01)。结论 大黄联合乌司他丁可通过降低百草枯中毒大鼠血浆TNF-α、TGF-β1水平,发挥对百草枯中毒大鼠肺组织的保护作用。
Abstract:
Objective To evaluate the intervention effect of Rhubarb combine with Ulinastatin on pathological changes of lung tissue and the plasma levels of TNF -α and TGF β1 content on paraquat poisoning induced lung injury in rats. Methods 72 SD rats were randomly divided into control group(n=8), phosgene group(n=32), intervention group(n=32). The rats in phosgene group and the intervention group were sacrificed at first days, 3 days, 5 days and 7 days. The changes of lung tissue were observed under light microscope, and the determination of the content of plasma TNF-α, TGF-β1. Results Lung tissue of phosgene group showed significant hemorrhage, inflammatory cell infiltration, pulmonary consolidation and other changes; The lung tissue of intervention group was better than the phosgene group; The level of TNF-α, TGF-β1 was increased on the first day after paraquat intoxication in phosgene group and intervention group, compared with control group, the result had statistical difference(P＜0.01); The plasma TGF-β1 and TNF-α of intervention group was lower than that of phosgene groups at the same time point, the differences were statistically significant(P＜0.01).Conclusions Rhubarb and Ulinastatin can reduce lung injury by decreasing the expression of TNF-α and TGF-β1 on paraquat poisoning induced lung injury in rats.

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备注/Memo

备注/Memo:
作者简介: 张永成(1976- ),本科,主治医师。研究方向:急性中毒。E-mail15666056239@163.com 大黄联合乌司他丁对百草枯中毒大鼠肺损伤保护作用
更新日期/Last Update: 2016-07-06