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[1]毛堂友 高康丽 赵唯含 王允亮 郭一 谢添弘 陈晨 谭祥 韩亚飞 李军祥.温运清利法对非酒精性脂肪性肝炎大鼠肝组织DAG-PKCε信号通路的作用研究[J].环球中医药,2016,9(08):908-913.[doi:10.3969/j.issn.1674-1749.2016.08.002]
　MAO Tang-you,GAO Kang-li,ZHAO Wei-han,et al.Experimental study of “Wenyun Qingli” method on DAG-PKCε signal pathway in liver tissue of NASH rats[J].,2016,9(08):908-913.[doi:10.3969/j.issn.1674-1749.2016.08.002]
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温运清利法对非酒精性脂肪性肝炎大鼠肝组织DAG-PKCε信号通路的作用研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年08期
页码:
908-913
栏目:
论著
出版日期:
2016-08-06

文章信息/Info

Title:
Experimental study of “Wenyun Qingli” method on DAG-PKCε signal pathway in liver tissue of NASH rats
作者:
毛堂友 高康丽 赵唯含 王允亮 郭一 谢添弘 陈晨 谭祥 韩亚飞 李军祥
Author(s):
MAO Tang-you;  GAO Kang-li;  ZHAO Wei-han;  et al.
Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China
关键词:
温运清利法;  非酒精性脂肪性肝炎;  DAG-PKCε信号通路;  作用研究
Keywords:
Wenyun Qingli method;  NASH;  DAG-PKCε signal pathway;  Experimental study
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.08.002
文献标志码:
A
摘要:
目的 探讨温运清利法对非酒精性脂肪性肝炎(non-alcoholic steatohepatitis,NASH)大鼠肝组织甘油二酯(diacylglycerol,DAG)/蛋白激酶Cε(protein kinase Cε,PKCε)信号通路的影响。方法 高脂饮食饲养SD大鼠8周制备NASH模型,造模成功后,将大鼠随机分为模型组、苓桂术甘汤组、茵陈蒿汤组、合方组(苓桂术等与茵陈蒿汤)、罗格列酮组,共5组,每组10只; 另有空白组10只。药物治疗4周后,运用自动生化分析仪检测血清生化指标,HE染色、油红O染色观察肝组织病理,ELISA法检测肝组织DAG,RT-PCR检测PKCε mRNA、TNF-α mRNA,Western blot检测PKCε和TNF-α蛋白的表达。结果(1)各给药组大鼠一般状况、肝组织脂肪变性和炎症反应程度、血清生化指标等均较模型组有一定的减轻;(2)苓桂术甘汤、合方组、罗格列酮组大鼠肝组织DAG较模型组明显降低(P＜0.05,P＜0.01);(3)各治疗组大鼠肝组织PKCε mRNA和蛋白含量均显著降低(P＜0.05,P＜0.01),茵陈蒿汤组、合方组及罗格列酮组大鼠肝组织TNF-α mRNA和蛋白含量显著降低(P＜0.05)。结论 温运清利法可能是通过下调肝组织DAG,抑制PKCε表达,降低TNF-α水平,从而对肝内脂质代谢、胰岛素抵抗、炎症损伤起到了调节作用,DAG/PKCε通路有可能成为治疗NASH的新靶点。
Abstract:
Objective To investigate the effect of Wenyun Qingli method on DAG-PKCε signal pathway in NASH rats. Methods High fat diet was used to induce NASH model on SD rats for 8 weeks. After 4 weeks of treatment, the automatic biochemical analyzer was used to measure the serum liver function(AST, ALT), blood lipid(TC, LDL, HDL, TG)level. HE staining and oil-red O staining method was used to observe the pathological changes of liver tissue, ELISA was used to detect the DAG of liver tissue,RT-PCR was used to detect the PKCε mRNA and TNF-α mRNA, Western blot was used to observe the expression of PKCε and TNF-α. Results(1)The mental state: general condition, liver index, inflammation and serum biochemical indices of treated groups were decreased compared with the model group;(2)The DAG levels of Linggui Zhugan decoction group, combination group, rosiglitazone group were decreased significantly compared with the model group(P＜0.05, P＜0.01);(3)The PKC mRNA and protein content of treated groups were decreased significantly compared with the model group(P＜0.05, P＜0.01), TNF-α mRNA and protein content in liver tissue of Yinchenhao decoction group, combination group, rosiglitazone group were significantly decreased(P＜0.05). Conclusion Wenyun Qingli method plays a role in protecting and treating NASH by down-regulating hepatic DAG and PKC expression, decreasing the levels of TNF alpha. DAG/ PKCε pathway may be a new target for treating NASH.

参考文献/References:

[1] 张会存,苏冬梅,刘莹,等. 二陈汤与苓桂术甘汤治疗非酒精性脂肪性肝病炎性损伤的机制研究[J].中国中西医结合消化杂志,2015,23(8):525-530.
[2] 刘莹,张会存,段娜,等. 茵陈蒿汤对高脂饮食诱导大鼠非酒精性脂肪性肝炎的药效学观察[J].中国实验方剂学杂志,2012,18(13):217-220.
[3] 刘莹,陈晓伟,李健,等. 茵陈蒿汤拆方对非酒精性脂肪性肝炎的正交实验研究[J].中国中西医结合消化杂志,2013,21(4):182-187.
[4] 苏冬梅,李健,李军祥,等.健脾疏肝方对非酒精性脂肪性肝炎大鼠ADP、TNF-α的影响[J].北京中医药大学学报,2011,34(12):826-831.
[5] 苏冬梅,诸葛丽,李军祥,等.健脾疏肝方对非酒精性脂肪性肝炎大鼠肝脂代谢分子网络的影响[J].世界华人消化杂志,2012,20(2):91-99.
[6] 王允亮,诸葛丽,李军祥,等.调肝解毒方对非酒精性脂肪性肝炎大鼠肝组织抗氧化能力的影响[J].吉林中医药,2012,32(10):1042-1045.
[7] Ong JP, Younossi ZM. Epidemiology and natural history of NAFLD and NASH[J].Clin Liver Dis,2007,11(1):1-16.
[8] Kumashiro N, Erion DM, Zhang D, et al. Cellular mechanism of insulin resistance in nonalcoholic fatty liver disease[J].PNAS,2011,108(39):16381-16385.
[9] Samuel VT, Liu ZX, Wang A, et al. Inhibition of protein kinase C epsilon prevents hepatic insulin resistance in nonalcoholic fatty liver disease[J].J Clin Invest,2007,117(3):739-745.
[10] Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance[J].Science,1993,259(5091):87-91.

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备注/Memo

备注/Memo:
基金项目: 高等学校博士学科点专项科研基金(20130013110007); 北京中医药大学自主选题项目(2015-JYB-XS172)
作者简介:毛堂友(1989- ),2014级在读博士研究生。研究方向:中医药防治胃肠疾病的研究。E-mail:maotangyouqun@126.com
通讯作者:李军祥(1964- ),博士,主任医师,博士生导师。研究方向:中医药防治慢性肝胆和胃肠道疾病。E-mail:lijunxiang1226@163.com
更新日期/Last Update: 2016-08-06