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[1]文谦 李芳梅 杨志波.当归饮子对银屑病模型豚鼠神经酰胺含量、AQP-3基因及蛋白表达的干预研究[J].环球中医药,2016,9(08):914-917.[doi:10.3969/j.issn.1674-1749.2016.08.003]
　WEN Qian,LI Fang-mei,YANG Zhi-bo..The intervention study of Danggui Yinzi decoction on the expression of gene and protein of AQP-3 and the content of ceramide in the posriasis vulgaris model of guinea pig[J].,2016,9(08):914-917.[doi:10.3969/j.issn.1674-1749.2016.08.003]
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当归饮子对银屑病模型豚鼠神经酰胺含量、AQP-3基因及蛋白表达的干预研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年08期
页码:
914-917
栏目:
论著
出版日期:
2016-08-06

文章信息/Info

Title:
The intervention study of Danggui Yinzi decoction on the expression of gene and protein of AQP-3 and the content of ceramide in the posriasis vulgaris model of guinea pig
作者:
文谦 李芳梅 杨志波
830000 乌鲁木齐,新疆医科大学附属中医医院皮肤科(文谦); 湖南中医药大学研究生院(李芳梅); 湖南中医药大学第二附属医院中医外科皮肤科(杨志波)
Author(s):
WEN Qian; LI Fang-mei; YANG Zhi-bo.
Department of Dermatological,Affiliated Hospital of Chinese Medicine,Xinjiang Medical University,Urumqi 830000,China
关键词:
当归饮子;  寻常型银屑病;  动物模型;  皮肤屏障功能
Keywords:
Danggui Yinzi;  Posriasis vulgaris;  Animal model;  Skin barrier function
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.08.003
文献标志码:
A
摘要:
目的 探讨当归饮子对寻常型银屑病模型豚鼠皮肤神经酰胺(ceramide,Cer)含量、水通道蛋白3(aquaporin 3,AQP-3)基因及蛋白表达的干预影响。方法 建立寻常型银屑病动物模型,分为当归饮子组、甲氨蝶呤组、生理盐水组、模型对照组、空白对照组,每组8只,酶联免疫吸附实验(ELISA)法检测造模皮损部位Cer含量,逆转录PCR(RT-PCR)和免疫印迹试验(Western blot)法检测造模皮损部位AQP-3基因及蛋白表达,分析寻常型银屑病动物模型皮损与皮肤屏障功能密切相关的基因及蛋白表达的变化。结果 豚鼠耳部皮肤银屑病模型造模成功。模型对照组皮损中ELISA法检测Cer含量,以及RT-PCR和Western blot法检测AQP-3基因的蛋白表达均较低; 当归饮子组与甲氨蝶呤组和各组中Cer含量、AQP-3基因蛋白表达增强(P＜0.01),且当归饮子组较甲氨蝶呤组增强明显(P＜0.01)。结论 当归饮子能增强银屑病模型豚鼠皮肤Cer含量及AQP-3基因和蛋白表达,对改善银屑病动物模型的皮肤屏障功能作用确切,效果优于甲氨蝶呤组。
Abstract:
Objective To investigate the effect of Danggui Yinzi on the expression of AQP-3 gene and Cer protein of psoriasis model. Methods To establish an animal model of psoriasis vulgaris. The animals were divided into Danggui Yinzi group,methotrexate group,saline group,model control group and blank control group. Aquaporin 3(AQP-3)of skin lesions part was detected with RT-PCR and Western blot methods and ELISA methods was used to detect the ceramide content of skin lesions part. Compared the results of the five groups,and the relationship of the expression changes of gene and protein in skin lesions and skin barrier function was analysed. Results The animal models of psoriasis vulgaris was successful In model control group,the content of ceramide and the expression of gene and protein of AQP-3 were all decreased significantly(P＜0.01). The expression index was increased in Danggui Yinzi group and methotrexate group and Danggui Yinzi group was increased obviously(P＜0.01). Conclusion Danggui Yinzi can enhance the expression of gene and protein of AQP-3 and the protein content of ceramide in skin lesions of psoriasis vulgaris model. It has a definite effect for skin barrier function of psoriasis vulgaris in animal model. The results indicate that the therapeutic effect is more effective than methotrexate medicine.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 湖南省科技计划(2011FJ3026)
作者简介: 文谦(1968- ),硕士,主治医师。研究方向:中医药防治皮肤病和性病的理论与临床研究。E-mail:wenq828@163.com
通讯作者: 杨志波(1956- ),硕士,主任医师,教授,博士生导师。研究方向:中西医结合防治皮肤病研究。E-mail:dr.yang888@126.com
更新日期/Last Update: 2016-08-06