[1]周建国 邬兰 马双姣 石林春 金钺 姚辉.基于ITS2序列的荆芥及其混伪品的DNA条形码鉴定[J].环球中医药,2016,9(08):923-927.[doi:10.3969/j.issn.1674-1749.2016.08.005]
ZHOU Jian-guo,WU Lan,MA Shuang-jiao,et al.Identification of Schizonepeta tenuifolia Briq. and its adulterants based on ITS2 sequence by DNA barcoding method[J].,2016,9(08):923-927.[doi:10.3969/j.issn.1674-1749.2016.08.005]
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基于ITS2序列的荆芥及其混伪品的DNA条形码鉴定(
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ZHOU Jian-guo,WU Lan,MA Shuang-jiao,et al.Identification of Schizonepeta tenuifolia Briq. and its adulterants based on ITS2 sequence by DNA barcoding method[J].,2016,9(08):923-927.[doi:10.3969/j.issn.1674-1749.2016.08.005]
)《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]
- 卷:
- 第9卷
- 期数:
- 2016年08期
- 页码:
- 923-927
- 栏目:
- 论著
- 出版日期:
- 2016-08-06
文章信息/Info
- Title:
- Identification of Schizonepeta tenuifolia Briq. and its adulterants based on ITS2 sequence by DNA barcoding method
- 作者:
- 周建国 邬兰 马双姣 石林春 金钺 姚辉
- 100193 中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室[周建国(硕士研究生)、马双姣(硕士研究生)、石林春、金钺、姚辉]; 中国中医科学院中药研究所[邬兰(博士研究生)]
- Author(s):
- ZHOU Jian-guo; WU Lan; MA Shuang-jiao; et al.
- The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
- 分类号:
- R282.5
- 文献标志码:
- A
- 摘要:
- 目的 通过分析裂叶荆芥及其混伪品的ITS2条形码序列,探索鉴定荆芥、荆芥穗及其混伪品的新方法,以保证荆芥的质量及临床疗效。 方法 对裂叶荆芥样品进行DNA提取、PCR扩增ITS2片段和双向测序,所有序列用软件MEGA 6.0进行相关数据分析,对裂叶荆芥及其混伪品间的种间序列差异进行分析比较,计算其种间、种内K2P遗传距离,并构建邻接树。结果 裂叶荆芥ITS2序列长度为232 bp,G+C含量为66.8%,序列高度保守,种内无变异位点。裂叶荆芥与其混伪品种间变异位点较多,种间K2P最小距离为0.0175,大于其种内距离,NJ树显示可以将裂叶荆芥与其混伪品完全分开。结论 ITS2序列可鉴别荆芥、荆芥穗及其混伪品,为荆芥的种质资源鉴定和保证临床用药安全提供了良好的技术手段。
- Abstract:
- Objective This study aimed to explore a new method to identify the original plant of Schizonepeta tenuifolia Briq. and its adulterants by the ITS2 sequence, and assure its quality and clinical curative effect. Methods Genomic DNA of S. tenuifolia were extracted and then the ITS2 sequences were obtained by direct PCR amplification and sequenced bidirectionally. All data of sequences were analyzed by software MEGA 6.0, the Kimura 2-Parameter(K2P)distances were calculated, and the phylogenetic tree was constructed by the neighbor joining(NJ)method. Results The sequence length of the ITS2 of Schizonepeta tenuifolia was 232bp, and the G+C content was 66.8%. The intra-specific variation was highly conservative. There were many variation sites of ITS2 sequences between Schizonepeta tenuifolia Briq. and its adulterants. The results showed that the minimum inter-specific divergence was 0.0175, which was larger than the maximum intra-specific divergence. The NJ tree indicated that Schizonepeta tenuifolia could be distinguished from its adulterants obviously. Conclusion The ITS2 region can be used as a barcode for identification of Schizonepeta tenuifolia Briq., which provide a new technique for the authentication of germplasm resouces and ensure clinical safety in utilization of traditional Chinese medicine.
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备注/Memo
- 备注/Memo:
-
基金项目: 重大新药创制国家科技重大专项(2014ZX09304307001); 国家科技支撑计划(2011BAI07B08)
作者简介: 周建国(1993- ),2015级在读硕士研究生。研究方向:中药资源与分子鉴定 。E-mail:1312905813@qq.com
通讯作者: 姚辉(1976- ),博士,研究员。研究方向:中药资源学。E-mail:scauyaoh@sina.com
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