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[1]王毓国,窦永起,秦丽.黛黄芍药散对放射性直肠炎大鼠COX-2、TXB2及6-Keto-PGF1α的影响[J].环球中医药,2016,9(09):1043-1046.[doi:10.3969/j.issn.1674-1749.2016.09.003]
　WANG Yu-guo,DOU Yong-qi,QIN Li..Effect of Daihuang Shaoyao powder on COX-2, TXB2 and 6-Keto-PGF1α in rats with radiation proctitis[J].,2016,9(09):1043-1046.[doi:10.3969/j.issn.1674-1749.2016.09.003]
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黛黄芍药散对放射性直肠炎大鼠COX-2、TXB2及6-Keto-PGF1α的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年09期
页码:
1043-1046
栏目:
论著
出版日期:
2016-09-06

文章信息/Info

Title:
Effect of Daihuang Shaoyao powder on COX-2, TXB2 and 6-Keto-PGF1α in rats with radiation proctitis
作者:
王毓国; 窦永起; 秦丽
100853 北京,中国人民解放军医学院[王毓国(硕士研究生)、窦永起、秦丽(硕士研究生)]
Author(s):
WANG Yu-guo;  DOU Yong-qi;  QIN Li.
Traditional Chinese Medicine Department of Chinese PLA General Hospital,Beijing 100853, China
关键词:
黛黄芍药散;  放射性直肠炎;  环氧化酶2;  血栓素B2;  6-酮-前列腺素F1α
Keywords:
Daihuang Shaoyao powder;  Radiation-induced proctitis;  Cyclooxygenase-2;  Thromboxane B2;  6-keto-prostaglandin F1α
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.09.003
文献标志码:
-
摘要:
目的 通过动物实验,研究中药复方黛黄芍药散在抑制微血管血栓形成、改善肠道微循环方面对放射性直肠炎的作用机制。方法 选用雌性Wistar大鼠60只,随机分为6组:空白对照组、模型组、地塞米松组及黛黄芍药散高、中、低剂量组,用60Co-γ射线单次盆腔照射制作大鼠放射性直肠炎模型,各组给予相应药物干预,在照射后第14天给药12小时后处死大鼠,取直肠进行免疫组化检测其环氧化酶2(cyclooxygenase-2,COX-2)的表达,采用酶联免疫吸附试验检测各组血清中血栓素B2(thromboxane B2,TXB2)、6-酮-前列腺素(6-keto-prostaglandin F1α,6-Keto-PGF1α)的浓度,并计算TXB2/6-Keto-PGF1α比值。结果 照射后第14天,模型组大鼠COX-2、TXB2含量及TXB2/6-Keto-PGF1α比值明显升高,而黛黄芍药散各剂量组中上述指标明显低于模型组(P＜0.01)。结论 黛黄芍药散可有效抑制放射性直肠炎大鼠肠道微血管血栓生成,改善微循环,其作用机制可能是通过控制COX-2的表达,从而减少TXB2的合成与释放,进而调节TXB2/6-Keto-PGF1α比值。
Abstract:
Objective To explore the mechanism of Daihuang Shaoyao powder on inhibiting thrombosis and improving the intestinal microcirculation in the rat model of radiation proctitis. Methods Sixty Wistar female rats were randomly divided into 6 groups, normal group, model group, dexamethasone group, high, mediumand, low dosage of Daihuang Shaoyao powder groups. Rat models of radiation-induced proctitis were produced by 60Co-γ ray radiation. The levels of COX-2, TXB2, 6-Keto-PGF1α and TXB2/6-Keto-PGF1α were detected after drugs intervention for 14 days. Results Compared with the model group, the levels of COX-2, TXB2 and TXB2/6-Keto-PGF1α were obviously decreased in high dosage of Daihuang Shaoyao powder(P＜0.01). Compared with the model group, Daihuang Shaoyao powder groups could effectively reduce the level of COX-2,TXB2 and TXB2/6-Keto-PGF1α (P＜0.01). Conclusion Daihuang Shaoyao powder could effectively inhibit the formation of intestinal microvascular thrombosis, improve microcirculation, and the mechanism of action may be through controlling the expression of COX-2, which can reduce the synthesis and release of TXB2, and then adjust the ratio of the TXB2/6-Keto-PGF1 α.

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备注/Memo

备注/Memo:
基金项目: 全军后勤科研计划(AWS14C014)
作者简介: 王毓国(1991- ),2014级在读硕士研究生。研究方向:放射性直肠炎的基础与临床研究。 E-mail:wangyuguo301@163.com
通讯作者: 窦永起(1965- ),硕士,教授,主任医师,博士生导师。研究方向:中医药治疗恶性肿瘤。E-mail: dyqi_301@yeah.net
更新日期/Last Update: 2016-09-06