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[1]陈珠,杨彩霞,倪婉晔,等.肾茶对急性痛风性关节炎大鼠的抗炎作用研究[J].环球中医药,2016,9(09):1051-1054.[doi:10.3969/j.issn.1674-1749.2016.09.005]
　CHEN Zhu,YANG Cai-xia,NI Wan-ye,et al.Anti-inflammation effect of Clerodendranthus spicatus on rats with acute gouty arthritis[J].,2016,9(09):1051-1054.[doi:10.3969/j.issn.1674-1749.2016.09.005]
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肾茶对急性痛风性关节炎大鼠的抗炎作用研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年09期
页码:
1051-1054
栏目:
论著
出版日期:
2016-09-06

文章信息/Info

Title:
Anti-inflammation effect of Clerodendranthus spicatus on rats with acute gouty arthritis
作者:
陈珠; 杨彩霞; 倪婉晔; 毛晓健; 赵荣华
650500 昆明,云南中医学院中药学院
Author(s):
CHEN Zhu;  YANG Cai-xia;  NI Wan-ye;  et al.
Yunnan University of Traditional Chinese Medicine,Kunming 650500,China
关键词:
肾茶;  急性痛风性关节炎;  抗炎
Keywords:
Clerodendranthus spicatus;  Acute gouty arthritis;  Anti-inflammation
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.09.005
文献标志码:
-
摘要:
目的 观察肾茶水煎剂对大鼠急性痛风性关节炎的抗炎作用。 方法 采用微晶型尿酸钠分别诱导大鼠急性踝关节肿胀和急性痛风性关节炎模型,以苯溴马隆为阳性对照,观察肾茶水煎剂对大鼠肿胀度的影响,并测定大鼠血清尿酸值(serum uric acid, SUA)及黄嘌呤氧化酶(xanthine oxidase, XOD)活性,ELISA试剂盒检测血清白细胞介素-1β(interleukin-1,IL-1β)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平。 结果 模型组关节肿胀度、IL-1β及IL-8水平明显高于正常对照组(P＜0.05),肾茶水煎剂高、中、低剂量能不同程度抑制大鼠踝关节肿胀度,降低血清IL-1β、IL-8水平(P＜0.05); TNF-α水平与模型组比较无显著性差异。同时肾茶水煎剂可显著降低大鼠血清SUA水平及XOD活性(P＜0.05或P＜0.01)。 结论 肾茶水煎剂对大鼠急性踝关节肿胀和急性痛风性关节炎有很好的控制作用,可能与其抑制尿酸生成、利尿以促进尿酸排泄、抑制炎症细胞因子IL-1β、IL-8表达有关。
Abstract:
Objective To observe the anti-inflammation effect of Clerodendranthus spicatus on rats with acute gouty arthritis(AGA). Methods Except the normal group, the others were given ankle injection with sodium urate crystal solution to induce the acute gouty arthritis model. The positive group were used the Benzbromarone, the influence of the aqueous extract of Clerodendranthus spicatus on rats ankle swelling, the level of serum uric acid and the activities of xanthine oxidase in the serum was observed. ELISA was used to detect serum IL-1β, IL-8 and TNF-α. Results Compared with normal group, the level of IL-1β and IL-8 was higher of AGA model group(P＜0.05, P＜0.01). High, middle and low dose of the aqueous extract of Clerodendranthus spicatus could significantly inhibit ankle swelling and reduce serum IL-1β and IL-8 leve1 at different degrees, reduce the level of serum uric acid(SUA)and the activities of xanthine oxidase(XOD)in the serum. Compared with the model group, the difference was significant(P＜0.05). Conclusion The aqueous extract of Clerodendranthus spicatus has obvious curative effect on acute gouty arthritis in rats. The therapeutic effect of AGA is related to decreasing in the levels of IL-1β、IL-8 in the serum.It can effectively inhibit the activities of xanthine oxidase, and decrease the formation of uric acid and promote the excretion of uric acid.

参考文献/References:

[1] 南京中医药大学. 中药大辞典[M]. 上海:上海科学技术出版社, 2006: 3081-3082.
[2] Zou J, Zhu YD, Zhao WM. Two new alkyl glycosides from Clerodendranthus spicatus[J]. J Asian Nat Prod Res, 2008, 10(7-8): 603-607.
[3] 任文辉,洪俪芳.猫须草的药理作用研究进展[J].中草药,2013, 44(20): 2946-2950.
[4] 陈奇. 中药药效研究思路于方法[M]. 北京:人民卫生出版社, 2005: 651-660.
[5] 李迎春, 徐建华. 急性痛风性关节炎的发病机制及研究进展[J]. 安徽医学, 2013, 34(1): 96-98.
[6] 金沈锐, 郑军, 刘绍唐. 小鼠高尿酸症模型初探[J]. 成都中医药大学学报, 1999, 22(1): 49-50.
[7] 王坤. 排石疗法在痛风防治中的运用初探[J]. 四川中医, 2013, 31(4): 29-30.
[8] Chen CJ, Shi Y, Hearn A, et al. MyD88-dependent IL-1 receptor signaling is essential for goulty inflammation stimulated by monosodium urate crystals[J].J Clin Invest,2006,116(8): 2262-2271.

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备注/Memo

备注/Memo:
基金项目: 云南省高校南药协同创新项目(30270101400)
作者简介: 陈珠(1989- ),女,2014级在读硕士研究生。研究方向:临床中药学。E-mail:1225493904@qq.com
通讯作者: 毛晓健(1967- ),女,硕士,教授,硕士生导师。研究方向:临床中药学、中药药理。E-mail:1224346204@qq.com
更新日期/Last Update: 2016-09-06