«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第9卷

期数:

2016年10期

页码:

1190-1193

栏目:

论著

出版日期:

2016-10-06

文章信息/Info

Title:

Influence of variation on Dahuang Zhechong pill on PDGF protein and PDGFR protein in liver of rats with immune hepatic fibrosis

作者:

王金光 车念聪 李文新 杜宇琼 司远 刘晔 赵晖

100069 北京,首都医科大学中医药学院中医临床基础学系[王金光(硕士研究生)、车念聪、李文新(硕士研究生)、杜宇琼、司远(硕士研究生)、刘晔(硕士研究生)、赵晖]

Author(s):

WANG Jin-guang;  CHE Nian-cong;  LI Wen-xin; et al.

School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China

关键词:

大黄虫丸;  免疫性肝纤维化;  血小板源性生长因子;  血小板源性生长因子受体

Keywords:

variation on Dahuang Zhechong pill;  Immune hepatic fibrosis;  PDGF;  PDGFR

分类号:

R249

DOI:

10.3969/j.issn.1674-1749.2016.10.007

文献标志码:

A

摘要:

目的 观察大黄虫丸配伍黄芪、水红花子汤剂对免疫性肝纤维化大鼠肝组织血小板源性生长因子(platelet derived growth factor,PDGF)及血小板源性生长因子受体(platelet derived growth factor receptor,PDGFR)的影响。 方法 将SD大鼠随机分为正常组、模型组、中药组和秋水仙碱组。除了正常组外的3组均建立猪血清腹腔注射诱导的免疫性肝纤维化模型。灌胃治疗6周后,取大鼠肝脏进行病理HE染色及通过碱水解法检测羟脯氨酸含量,并通过蛋白免疫印迹法检测肝组织PDGF-BB蛋白及PDGFR蛋白的表达。 结果 与正常组相比,模型组羟脯氨酸含量、PDGF-BB蛋白及PDGFR蛋白的表达均明显增加(P<0.01); 与模型组相比,中药组及秋水仙碱组羟脯氨酸含量减少(P<0.05),PDGF-BB蛋白及PDGFR蛋白的表达明显减少(P<0.01); 中药组与秋水仙碱组羟脯氨酸含量、PDGF-BB蛋白及PDGFR蛋白的表达均未见明显差异(P>0.05)。 结论 大黄虫丸配伍黄芪、水红花子能有效拮抗猪血清诱导的大鼠免疫性肝纤维化,其机制可能与抑制PDGF-BB及受体PDGFR蛋白的表达,进而抑制肝星状细胞的增殖活化有关。

Abstract:

Objective To investigate the influence of variation on Dahuang Zhechong pill combine with mikvetch root and prince's-feather fruit decoction on PDGF protein and PDGFR protein of rats with hepatic fibrosis. Methods SD rats were randomly divided into normal group, model group and western medicine, Chinese medicine group. Except the normal group, the other 3 groups were induced liver fibrosis model by intraperitoneal injection of porcine serum, after six weeks of treatment,pathology HE of rat liver was conducted and alkali hydrolysis method was used to decect hydroxyproline content, the expression of PDGF - BB protein and PDGFR protein was detected by protein immunoblot method. Results Compared with the normal group, hydroxyproline content,PDGF-BB proteins and PDGFR protein expression of model group was significantly increased(P<0.01); Compared with model group, the hydroxyproline content in Chinese medicine group and colchicine group was decreased(P<0.05), the expression of PDGF-BB protein and PDGFR protein was decreased significantly(P<0.01); the hydroxyproline content, the expression of PDGF-BB protein and PDGFR protein between Chinese medicine group and colchicine group had no significant difference(P>0.05). Conclusion variation on Dahuang Zhechong pill combine with mikvrtch root and prince's-feather fruit decoction can antagonism liver fibrosis induced by porcine serum, the mechanism may be related to the inhibition of PDGF-BB and PDGFR protein expression, and the inhibition of the hepatic stellate cell activation.

参考文献/References:

[1] 刘瑶. 早期乙肝肝硬化中医证素特征及其与血浆CTGF、PDGF的相关性研究[D]. 福州:福建中医药大学, 2014.
[2] 李川, 吕文良. 常见中药复方制剂抗肝纤维化机制研究进展[J]. 环球中医药, 2013, 6(1): 70-73.
[3] 李康, 党诚学.大黄蛰虫丸药理作用及临床应用[J].现代中西医结合杂志,2004,13(11):1440.
[4] 陈红. 中西医结合治疗肝硬化腹水近况[J].广西中医学院学报,2005, 8(1): 65.
[5] 杜宇琼,车念聪. 黄芪对肝纤维化大鼠肝组织TIMP-1及MMP-1表达的影响[J]. 北京中医药大学学报, 2013, 36(11): 775-778.
[6] 杜宇琼,车念聪. 水红花子对免疫肝纤维化大鼠肝功能及血清肝纤维化标志物的影响[J].中国中医急症, 2011, 20(9):1433-1434.
[7] 翟延君, 张淑荣, 郝宁. 水红花子研究概况[J].辽宁中医学院学报, 2005, 7(3): 226.
[8] Nagashio Y, Ueno H, Imamura M. Inhibition of transforming growth factor beta decreases pancreatic fibrosis and protects the pancreas against chronic injury inmice[J]. Lab Invest, 2004, 84(12):1610-1618.
[9] Bonner JC. Regulation of PDGF and its receptors in fibrotic diseases[J].Cytokine Growth Factor Rev,2004,15(4):255-273.
[10] 白辰,车念聪,刘文兰.一贯煎汤剂对肝纤维化大鼠肝脏1型胶原的影响[J]. 环球中医药, 2015, 8(8): 901-903.

相似文献/References:

备注/Memo

备注/Memo:

基金项目: 2015年度首都中医药研究专项(15ZY01); 2015年度首都医科大学校自然基金(2015ZR25)
作者简介: 王金光(1987- ),2014 级在读硕士研究生。研究方向:中医肝病的证治规律研究。E-mail:jinguang1213@163.com
通讯作者: 车念聪(1957- ),教授,博士生导师,主任医师。研究方向:中医肝病。E-mail: cnc088@ sohu.com

常用功能

更新日期/Last Update: 2016-10-06