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[1]叶迎 包强 王瑞海 柏冬 刘卫东 彭建平 陈永刚 田生华 刘培元 杨丽霞 姜良恩 刘丽梅.甘肃黄芪和红芪中总皂苷含量测定对比研究[J].环球中医药,2016,9(10):1197-1203.[doi:10.3969/j.issn.1674-1749.2016.10.009]
　YE Ying,BAO Qiang,WANG Rui-hai,et al.Comparative study on content of total saponins of Astragalus and Radix Hedysari in Gansu[J].,2016,9(10):1197-1203.[doi:10.3969/j.issn.1674-1749.2016.10.009]
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甘肃黄芪和红芪中总皂苷含量测定对比研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年10期
页码:
1197-1203
栏目:
论著
出版日期:
2016-10-06

文章信息/Info

Title:
Comparative study on content of total saponins of Astragalus and Radix Hedysari in Gansu
作者:
叶迎 包强 王瑞海 柏冬 刘卫东 彭建平 陈永刚 田生华 刘培元 杨丽霞 姜良恩 刘丽梅
100700 北京,中国中医科学院中医基础理论研究所中药分析室[叶迎(硕士研究生)、王瑞海、柏冬、刘丽梅]; 甘肃省中医院药学部(包强、杨丽霞、姜良恩); 甘肃省宕昌县中药材产业开发中心(刘卫东、彭建平、陈永刚); 甘肃省陇南市武都区中药材中心(田生华、刘培元)
Author(s):
YE Ying; BAO Qiang; WANG Rui-hai; et al.
Institute of Basic Theory Research of TCM,China Academy of Chinese Medical Sciences,Beijing 100700,China
关键词:
黄芪;  红芪;  总皂苷;  紫外-可见分光光度法;  含量测定
Keywords:
Astragalus;  Radix Hedysari;  Total saponins;  Ultraviolet visible spectrophotometry;  Content determination
分类号:
R284.1
DOI:
10.3969/j.issn.1674-1749.2016.10.009
文献标志码:
A
摘要:
目的 建立甘肃不同产地黄芪和红芪药材中总皂苷含量测定的方法,比较不同产地黄芪和红芪总皂苷含量。方法 以黄芪甲苷为对照品,采用香草醛-高氯酸显色法,检测波长为540 nm,测定甘肃5县1区28批黄芪和28批红芪样品中总皂苷的含量。结果 黄芪甲苷对照品质量在0.0498～0.1638 mg范围内与吸光度线性关系良好(r=0.9993),黄芪和红芪的平均加样回收率分别为96.87%和98.96%,相对标准偏差分别为2.00%和2.86%(n=6)。甘肃各地区黄芪样品中总皂苷平均含量分别为宕昌5.10%、武都4.41%、陇西等3地4.21%; 红芪样品中总皂苷平均含量分别为宕昌3.41%、武都4.15%、陇西等4地3.06%。结论 本研究建立的黄芪和红芪总皂苷含量测定方法简便、准确,重复性好,可用于甘肃地区黄芪和红芪中总皂苷含量测定及其对比研究。甘肃不同产地黄芪和红芪药材中总皂苷含量存在差异,宕昌黄芪总皂苷含量最高,武都红芪总皂苷含量最高,且同一地区黄芪总皂苷含量高于红芪总皂苷。
Abstract:
Objective To establish a method for determining the total saponins content of Astragali and Radix Hedysari in Gansu Province, and compare the total saponins content of Astragali and Radix Hedysari in different areas of Gansu Province. Methods The vanillin-perchloric acid colorimetric method was used to detect the total saponins content of 28 batches of Radix Astragali and 28 batches of Radix Hedysari samples which were got from five regions of Gansu Province, Astragalus saponins was used as the control sample and 540 nm was chosen as the detection wavelength. Results The absorbance and the concentration had a good linear relationship(r=0.9993), and the absorbance of astragaloside IV was linear in range of 0.0498～0.1638 mg.The average recoveries of Astragali and Radix Hedysari were 96.87% and 98.96% respectively, the RSD were 2.00% and 2.86%(n=6)respectively. The average content of the total saponins in the sample of Astragalus in Tangchang was 5.10%, Wudu was 4.41%, Longxi and another two areas were 4.21%. The average content of total saponins of Radix Hedysari samples in Tangchang was 3.41%, Wudu was 4.15%, Longxi and another two areas were 3.06%. Conclusion The method for detecting the total saponins content of Astragali and Radix Hedysari in Gansu Province was simple, accurate and reproducible, and can be used for the comparative study on the content of total saponins of Astragalus and Radix Hedysari. There are differences in the content of total saponins of Radix Astragali and Radix Hedysari in Gansu Province. The total saponin content of Astragalus in Wudu is highest, and the total saponin content of Radix Hedysari in Tangchang was the highest. Total saponin content of Astragalus was higher than that of Radix Hedysar in the same area of Gansu Province.

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备注/Memo

备注/Memo:
基金项目: 中国中医科学院自主选题(YZ-1438)
作者简介: 叶迎(1991- ),女,2014级在读硕士研究生。研究方向:质量标准研究。E-mail:1011153961@qq.com
通讯作者: 刘丽梅(1965- ),女,本科,研究员。研究方向:中药化学质量分析。E-mail:liulimeihrb@sina.com
更新日期/Last Update: 2016-10-06