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[1]丁劲 张耀圣 商建伟 李曰庆 柴立民 李宪瑞 盛文 韦翊 马健雄.益肾健脾方对少弱精子症小鼠模型睾丸生精功能和血清性激素水平的影响[J].环球中医药,2016,9(11):1310-1314.[doi:10.3969/j.issn.1674-1749.2016.11.007]
　DING Jin,ZHANG Yao-sheng,SHANG Jian-wei,et al.Influences of Yishen Jianpi recipe on spermatogenesis function and serum sex hormone level of the mice model of oligoasthenotspermia[J].,2016,9(11):1310-1314.[doi:10.3969/j.issn.1674-1749.2016.11.007]
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益肾健脾方对少弱精子症小鼠模型睾丸生精功能和血清性激素水平的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年11期
页码:
1310-1314
栏目:
论著
出版日期:
2016-11-06

文章信息/Info

Title:
Influences of Yishen Jianpi recipe on spermatogenesis function and serum sex hormone level of the mice model of oligoasthenotspermia
作者:
丁劲 张耀圣 商建伟 李曰庆 柴立民 李宪瑞 盛文 韦翊 马健雄
100700 北京中医药大学东直门医院男科[丁劲(硕士研究生)、张耀圣、商建伟、李曰庆、李宪锐(硕士研究生)、盛文(硕士研究生)、马健雄(硕士研究生)],中医内科教育部和北京市重点实验室[柴立民、韦翊(硕士研究生)]
Author(s):
DING Jin;  ZHANG Yao-sheng;  SHANG Jian-wei;  et al.
Male Department of Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700,China Corresponding author: SHANG Jian-wei, E-mail:sjw313@163.com
关键词:
益肾健脾方;  少弱精子症;  雷公藤多苷;  精液质量;  血清性激素
Keywords:
Yishen Jianpi recipe;  Oligoasthenotspermia;  Tripterygium glycosides;  Semen quality;  Serum sex hormone level
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.11.007
文献标志码:
A
摘要:
目的 观察益肾健脾方对雷公藤多苷诱导少弱精子症小鼠模型睾丸组织、精液质量和血清性激素水平的影响,探讨益肾健脾方治疗少弱精子症可能的作用机制。方法 以雷公藤多苷诱导少弱精子症小鼠模型,随机平均分为正常组、模型组、左卡尼汀组、中药组(益肾健脾方低、高剂量组),每组10只,中药及左卡尼汀干预,睾丸组织病理切片、HE染色,观察小鼠睾丸组织病理学,应用伟力精子测试仪检测小鼠精液质量,应用酶联免疫夹心法检测小鼠血清性激素水平。结果 左卡尼汀组、中药组的A级精子活力、总活力高于模型组(P<0.05); 左卡尼汀组低于中药低剂量组(P<0.05); 中药高剂量组与左卡尼汀组相比差异无统计学意义(P>0.05)。左卡尼汀组、中药组的精子总数高于模型组(P<0.05); 左卡尼汀组、中药低、高剂量组之间相比差异无统计学意义(P>0.05)。模型组小鼠血清卵泡刺激素、黄体生成素水平高于正常组及各药物干预组(P<0.05); 各药物干预组高于模型组(P<0.05); 各药物干预组之间相比差异无统计学意义(P>0.05); 模型组及各干预组血清睾酮低于正常组(P<0.05); 模型组与各干预组之间相比差异无统计学意义(P>0.05)。结论 益肾健脾方可能通过调节小鼠血清性激素水平从而改善精液质量达到治疗少弱精子症的目的。
Abstract:
Objective To observe the changes of the testis tissue, semen quality and serum sex hormone level in mice model of oligoasthenotspermia induced by tripterygium glycosides, and discuss the possible intervention mechanism of Yishen Jianpi recipe to oligoasthenotspermia.Methods Mice model of oligoasthenotspermia was induced by tripterygium glycosides and the mice were randomly divided into normal group, model group, L-carnitine group, Chinese medicine group(Yishen Jianpi recipe low-dose and high-dose group)each group inclucled 120 mice. Chinese medicine and L-carnitine were used for intervention, Pathological section HE staining and electron microscope were used to observe the pathological changes of tissue, Weili sperm tester were used to observe the semen quality, serum sex hormone levels were detected by enzyme linked immunosorbent assay. Results A level and total sperm motility in the L-carnitine group and Chinese medicine group were higher than those in the model group(P<0.05); the L-carnitine group were lower than the low-dose group(P<0.05); compared with the L-carnitine group, the high-dose group was not significant difference(P>0.05). Sperm total in the L-carnitine group and Chinese medicine group were higher than that in the model group(P<0.05); among the L-carnitin group, low-dose and high-dose group, there were no statistical significance(P>0.05). Serum follicle stimulating hormone and lutein level in other group were higher than that in the normal group(P<0.05); intervention groups were higher than model group(P<0.05); there was no significance difference between model group and intervention groups(P>0.05). There was no significance difference in serum T level between the model group and intervention groups(P>0.05); the model group and intervention group were lower than that in the normal group(P<0.05). Conclusion Yishen Jianpi recipe can regulate the level of serum sex hormone in mice model of oligoasthenotspermia and affect the occurrence of sperm to achieve the goal of improving the quality of semen in the treatment of oligoasthenotspermia.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81273756); 北京中医药大学中青年教师资助项目(2015-JYB-JBMS062)
作者简介: 丁劲(1988- ),2013级在读硕士研究生。研究方向:中西医结合防治男性疾病。E-mail:djdoctor203@163.com
通讯作者: 商建伟(1983- ),2015级在读博士研究生,主治医师。研究方向:中西医结合治疗男性疾病。E-mail:sjw313@163.com
更新日期/Last Update: 2016-11-06