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[1]闫瑢玓 方慧 迟莉.灯盏花乙素对肝星状细胞及TGF-β1/Smad信号通路的作用评价[J].环球中医药,2016,9(11):1314-1317.[doi:10.3969/j.issn.1674-1749.2016.11.008]
　YAN Rong-di,FANG Hui,CHI Li..Effect of scutellarin on TGF-β1/Smad signaling pathway in hepatic stellate cell[J].,2016,9(11):1314-1317.[doi:10.3969/j.issn.1674-1749.2016.11.008]
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灯盏花乙素对肝星状细胞及TGF-β1/Smad信号通路的作用评价()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年11期
页码:
1314-1317
栏目:
论著
出版日期:
2016-11-06

文章信息/Info

Title:
Effect of scutellarin on TGF-β1/Smad signaling pathway in hepatic stellate cell
作者:
闫瑢玓 方慧 迟莉
250001 济南,山东中医药大学第二附属医院职业病科(闫瑢玓); 首都医科大学中医药学院中医基础学系[方慧(本科生)、迟莉]
Author(s):
YAN Rong-di; FANG Hui; CHI Li.
Department of Occupational Disease, the Second Affiliated Hospital of Shandong University of Chinese Medicine,Jinan 250001,China
关键词:
灯盏花乙素;  肝星状细胞;  TGF-β1/Smad信号通路
Keywords:
Scutellarin;  Hepatic stellate cell;  TGF-β1 /Smad signaling pathway
分类号:
R285
DOI:
10.3969/j.issn.1674-1749.2016.11.008
文献标志码:
A
摘要:
目的 观察灯盏花乙素(scutellarin)对肝星状细胞(hepatic stellate cell,HSC)及转化生长因子β1(TGF-β1)/Smad信号通路的影响,探讨scutellarin抗肝纤维化的机制。 方法 建立TGF-β1诱导的HSC LX-2模型,CCK-8法检测scutellarin对细胞增殖的作用,化学发光法检测scutellarin对Caspase3/7活性的影响,酶联免疫吸附测定(enzyme linked immunosorbent assay, ELISA)法检测细胞上清液Ⅰ型前胶原的含量,ELISA法检测细胞Smad2和Smad3磷酸化水平。 结果 scutellarin能抑制HSC增殖,增强细胞Caspase3/7活力,抑制Ⅰ型前胶原分泌,降低LX-2细胞Smad2和Smad3磷酸化水平。 结论 抑制TGF-β1/Smad信号通路可能是scutellarin抗肝纤维化的作用机制。
Abstract:
Objective To observe the effect of scutellarin on signaling pathway in hepatic stellate cells(HSC)and transforming growth factor beta 1(TGF- beta 1), to explore the mechanisms of anti-hepatic fibrosis. Methods Experiments were conducted by human HSC cells line. Transforming growth factor-β1(TGF-β1)was used to induce HSC model. The inhibitory effect of scutellarin on LX-2 cells was observed. Chemiluminescence measurement was used to measure the activity of caspase3/7 in LX-2 cells. ELISA was used to detect the content of pro-collagenⅠin LX-2 supernatant. The expressions of Phospho-Smad2 and Phospho-Smad3 were measured by ELISA. Results Scutellarin could Inhibit LX-2 cells proliferation, pro-collagen, Ⅰsecretion, and phosphorylation of Smad2, Smad3 expressions. Scutellarin could improve the activities of caspase3/7. Conclusion The mechanism of scutellarin to anti hepatic fibrosis may be the inhibition of TGF- beta 1/Smad signaling pathway

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 2015年首都中医药研究专项(15ZM08)
作者简介: 闫瑢玓(1978- ),女,硕士,主治医师。研究方向:职业性疾病和中毒性疾病的诊治。E-mail:kellycat813@163.com
通讯作者: 迟莉(1980- ),女,博士,讲师。研究方向:肝脏疾病的文献和实验研究。E-mail:13911824903@163.com
更新日期/Last Update: 2016-11-06