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[1]王永强,师一民,安辰,等.补肾益髓方促进实验性自身免疫性脑脊髓炎小鼠髓鞘再生的作用[J].环球中医药,2016,9(12):1443-1449.[doi:10.3969/j.issn.1674-1749.2016.12.002]
　WANG Yong-qiang,SHI Yi-min,AN Chen,et al.Effects of Bushen Yisui formula on promoting remyelination in mice with experimental autoimmune encephalomyelitis[J].,2016,9(12):1443-1449.[doi:10.3969/j.issn.1674-1749.2016.12.002]
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补肾益髓方促进实验性自身免疫性脑脊髓炎小鼠髓鞘再生的作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年12期
页码:
1443-1449
栏目:
论著
出版日期:
2016-12-06

文章信息/Info

Title:
Effects of Bushen Yisui formula on promoting remyelination in mice with experimental autoimmune encephalomyelitis
作者:
王永强; 师一民; 安辰; 赵晖; 李明; 齐放; 张秋霞; 陈振振; 樊永平; 王蕾
100069 北京,首都医科大学中医药学院中医络病研究北京市重点实验室[王永强(硕士研究生)、师一民(硕士研究生)、安辰(硕士研究生)、赵晖、李明、齐放、张秋霞、陈振振、王蕾]; 首都医科大学附属北京天坛医院中医科(樊永平)
Author(s):
WANG Yong-qiang;  SHI Yi-min; AN Chen; et al.
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, China
关键词:
补肾益髓方;  实验性自身免疫性脑脊髓炎;  髓鞘再生
Keywords:
Bushen Yisui formula;  Experimental autoimmune encephalomyelitis;  Remyelination
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.12.002
文献标志码:
A
摘要:
目的 观察补肾益髓方促进实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)小鼠髓鞘再生的作用。方法 80只雌性C57BL/6小鼠随机分为正常对照、模型、醋酸泼尼松及补肾益髓方组。以髓鞘少突胶质细胞糖蛋白(myelin oligodendroglia glycoprotein,MOG)35-55诱导小鼠EAE模型。治疗组小鼠每天给予相应药物灌胃治疗,共40天,醋酸泼尼松为阳性对照药物。每天观察动物的一般情况,记录小鼠神经功能评分,统计小鼠的发病率、潜伏期、病程和疾病负荷等。取小鼠脑和脊髓经固蓝染色观察其髓鞘变化,免疫组化法测定髓鞘再生相关蛋白硫酸软骨素蛋白多糖2胶质细胞和髓鞘碱性蛋白(myelin basic protein,MBP)的表达。结果 补肾益髓方能够延长小鼠发病潜伏期、缩短EAE病程、降低小鼠疾病负荷(P＜0.05),减少小鼠脑和脊髓内炎细胞浸润和髓鞘脱失; 本方还可促进NG2和MBP蛋白的表达(P＜0.01)。结论 补肾益髓方对EAE小鼠有神经保护作用,并能减轻髓鞘损伤和促进髓鞘修复。
Abstract:
Objective To observe the effect of Bushen Yisui formula on promoting remyelination in mice with experimental autoimmune encephalomyelitis(EAE). Methods 80 female C57BL/6 mice were randomly divided into normal control, model, prednisone acetate and Bushen Yisui formula-treated groups. EAE model was established through the injection of myelin oligodendroglia glycoprotein(MOG)35-55. The mice in treatment groups were administrated administration with corresponding medicines daily for 40 days. Prednisone acetate was considered as the positive control. Animal condition was observed and neurofunctional score was recorded every day. Meanwhile, the morbidity, latency, disease course and disease burden were acquired. Luxol fast blue staining of the brain and spinal cord was performed to observe the changes of myelin. The expressions of myelin regeneration associated proteins chondroitin sulfate proteoglycan 2 and myelin basic protein(MBP)were determined by the immunohistochemistry technique. Results Bushen Yisui formula could prolong the latent period, shorten the course, lighten the disease burden(P＜0.05).It could reduce inflammatory cell infiltration and demyelination in the brain and spinal cord of EAE mice. Bushen Yisui could also improve the protein expressions of NG2 and MBP(P＜0.01). Conclusion Bushen Yisui had neuroprotective effects on EAE mice. It was able to alleviate myelin injury and promote the recovery of myelin.

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相似文献/References:

[1]樊永平 杨涛 郑琦等.痰热清注射液对实验性变态反应性脑脊髓炎小鼠炎性因子及炎性损伤影响的研究[J].环球中医药,2014,7(07):501.
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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81273742、81573898); 北京市属高等学校高层次人才引进与培养计划-长城学者项目(CIT&TCD20140329)
作者简介: 王永强(1989- ),2014级在读硕士研究生。研究方向:中医药防治脑病研究。E-mail:644750798@qq.com
通讯作者: 王蕾(1967- ),女,博士,教授,博士生导师。研究方向:中医药防治脑病研究。E-mail:tmwangl@ccmu.edu.cn
更新日期/Last Update: 2016-12-06