|本期目录/Table of Contents|

[1]吴越,朱峰,刘建平,等.四逆散对高脂血症小鼠血脂代谢途径的影响[J].环球中医药,2016,9(12):1450-1454.[doi:10.3969/j.issn.1674-1749.2016.12.003]
　WU Yue,ZHU Feng,LIU Jian-Ping,et al.Effects of Sini powder on blood lipid metabolism in mice with hyperlipidemia[J].,2016,9(12):1450-1454.[doi:10.3969/j.issn.1674-1749.2016.12.003]
点击复制

四逆散对高脂血症小鼠血脂代谢途径的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年12期
页码:
1450-1454
栏目:
论著
出版日期:
2016-12-06

文章信息/Info

Title:
Effects of Sini powder on blood lipid metabolism in mice with hyperlipidemia
作者:
吴越; 朱峰; 刘建平; 李永民
030024 太原,山西中医学院(吴越); 河北北方学院中医学院(朱峰、李永民); 中南大学湘雅二医院(刘建平)
Author(s):
WU Yue; ZHU Feng; LIU Jian-Ping; et al.
Shanxi University of Traditional Chinese Medicine,Taiyuan 030024,China
关键词:
四逆散;  高脂血症;  载脂蛋白A-I
Keywords:
Sini powder;  Hyperlipidemia;  Apolipoprotein A-1
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.12.003
文献标志码:
A
摘要:
目的 探讨四逆散对高脂血症C57BL/6小鼠脂代谢的影响并初步探讨其机制。方法 40只SPF级C57BL/6小鼠作为研究对象,随机分为对照组、模型组、四逆散(0.9 mg/g体质量)组、枳实(0.9 mg/g体质量)组,分别以生理盐水、四逆散水煎剂、枳实水煎剂灌胃,干预时间为90天。全自动生化分析仪检测血清甘油三脂(triglyceride,TG),低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C),高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、ELISA法检测血清载脂蛋白A-1(apolipoprotein A-1,APOA-Ⅰ)水平及实时定量反转录-聚合酶链反应检测肝脏以及小肠组织中APOA-I的mRNA基因表达情况。结果 在脂代谢方面,四逆散干预可显著防止高脂饮食小鼠血清TG、LDL-C水平的升高(P＜0.05),显著提升血清中HDL-C和APOA-I水平(P＜0.05),显著增加肝脏中APOA-I的mRNA表达程度(P＜0.05)。结论 四逆散可能是通过促进肝脏APOA-I mRNA表达和增加APOA-I分泌来干预血脂水平。
Abstract:
Objective To observe the effects of Sini powder on C57BL/6J mice with hyperlipidemia and explore its mechanism. Methods 40 C57BL/6 mice were randomly divided into blank control group,hyperlipidemia group,hyperlipidemia plus Sini powder(0.9 mg/g weight)group and hyperlipidemia plus fructus aurantii immaturus(0.9mg/g weight). Blank control group were fed with normal diet and the other groups were fed with high fat diet. Mice were received gavage administration with corresponding drug,Sini powder and fructus aurantii immaturus were intervened for 90 days. The automatic biochemistry analyzers method was used to measure the levels of TG,LDL-C,HDL-C in serum. Elisa methods were used to detect the APOA-I levels in serum. Real-time PCR methods were used to detect the APOA-I mRNA expression changes in liver and small intestine. Results Compared with hyperlipidemia group,Sini powder could decrease the levels of TG and LDL-C(P＜0.05),increase the levels of HDL-C and improve the level of APOA-I in serum(P＜0.05). The mRNA expressions of APOA-I in liver was increased in Sini powder combined hyperlipidemia group. Conclusion Sini powder may be to promote the expression of mRNA APOA-I in liver and increase APOA-I secretion to interfere with blood lipid level.

参考文献/References:

[1] kazuo Shigematsu,Yoshiyuki Watanabe,Hiromi. Nakano Influences of hyperlipidemia history on strokeoutcome; a retrospective cohort study based onthe Kyoto Stroke Registry[J].BMC Neurology,2015,15(1):1-6.
[2] Karcher HS,Holzwarth R,Mueller HP,et al. Body fat distribution as a risk factor for cerebrovascular disease:an MRI-basedbody fat quantification study[J].Cerebrovasc Dis,2013,35(4):341-348.
[3] Michael Demyen,Kawtar Alkhalloufi,Nikolaos T. Pyrsopoulos. Lipid-Lowering Agents and Hepatotoxicity[J].Clin Liver Dis,2013,17(4):699-714
[4] Seth S,Martin Erin D,Michos. Mapping Hyperlipidemia in Young Adulthood to Coronary Risk:Importance of Cumulative Exposure and How to Stay Young[J].Circulation,2015,131(5):445-447.
[5] 朴胜华,郭姣,胡竹平. 高脂血症住院患者中医证候临床研究[J].中国中西医结合杂志 2012,32(10):1322-1325.
[6] 张乔,金阳,徐瑞鑫,等.四逆散有效组分改善睡眠作用与5-羟色胺能神经系统相关性的实验研究[J].中国医药导报,2012,14(9):29-30.
[7] 陈华.医学实验动物学[M].北京:军事医学科学出版社,2013:270-273.
[8] Ma S,Yu H,Zhao Z,et al. Activation of the cold-sensing TRPM8channel triggers UCP1-dependent thermogenesis and prevents obesity[J]. J Mol Cell Biol,2012,4(2):88-96.
[9] 赵玉敏. 调畅气机在治疗高脂血症中的意义[J].中华中医药杂志,2005,20(8):488-489
[10] 黄金花,马俐,杜芬,等.甘草酸对动脉粥样硬化斑块的消退作用[J].中国医院药学杂志,2015,35(7):571-575.
[11] 吴东方,靳善睿,刘娟,等.芍药苷抗ApoE基因缺失小鼠动脉粥样硬化作用的初步研究[J].中国医院药学杂志,2015,35(5):385-388
[12] 邵淑丽,徐兴军,马德滨,等.柴胡、姜黄对小白鼠实验性高脂血症的预防作用[J].中医药学报,2002,30(4):59-60.
[13] 吴立华,吉中强,纪文岩,等.枳实对高脂血症患者血脂和内皮功能的影响[J].中西医结合心脑血管病杂志,2012,10(3):283-285
[14] 吴剑锋,唐艳艳,曾高峰,等. 载脂蛋白AI对HDL生成影响的研究新进展[J].生理科学进展,2014,45(2):81-86.
[15] Lagerstedt JO,Budamagunta MS,Liu GS,et al. The “beta-clasp” model of apolipoprotein A-I a lipid-free solutionstructure determined by electron paramagnetic resonance spectroscopy[J]. Biochim Biophys Acta,2012,1821(3):448-455.
[16] 蒋洁云,徐强.四逆散及其各组成中药对实验性肝损伤的影响[J].中国天然药物,2004,2(1):45-49
[17] 郑纯威,丁华熳,陈宇,等.柴胡皂苷改善大鼠肝纤维化的实验研究[J].中国中医急症,2011,20(5):755.
[18] 魏伟,刘家骏,刘家琴,等.白芍总苷对乙型肝炎的治疗作用及其前景[J].中国药理学通报,2000,16(5):596
[19] 焦士蓉,黄承钰,王波,等.枳实提取物对实验性糖尿病小鼠肝脏抗氧化防御功能的影响[J]. 卫生研究,2007,36(6):689-693.
[20] 陈云华,黄明进,王文全,等.不同来源甘草药材成分含量及其保肝降酶作用比较[J]. 中国实验方剂学杂志, 2013,18(19):113-116.

相似文献/References:

[1]官菊梅,高锋,安雪梅,等.李孔定教授四逆散临证新用述略[J].环球中医药,2015,8(05):590.[doi:10.3969/j.issn.16741749.2015.05.022]
[2]邢峰丽,封小强,刘伟花,等.荷叶的药理作用研究概述[J].环球中医药,2016,9(01):115.[doi:10.3969/j.issn.1674-1749.2016.01.036]
　XING Feng-li,FENG Xiao-qiang,LIU Wei-hua,et al.Review on research of lotus leaf's pharmacological effects[J].,2016,9(12):115.[doi:10.3969/j.issn.1674-1749.2016.01.036]

备注/Memo

备注/Memo:
基金项目: 河北北方学院创新人才培育基金(CXRC1319); 河北省中医药管理局课题(2015171)
作者简介: 吴越(1991- ),2014级在读硕士研究生。研究方向:中医药防治心血管疾病。E-mail:dawn_233@163.com
通讯作者: 李永民(1970- ),博士,教授。研究方向:中医内科学、中药药理学。E-mail:liyongmin2001@sina.com
更新日期/Last Update: 2016-12-06