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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第9卷

期数:

2016年12期

页码:

1454-1458

栏目:

论著

出版日期:

2016-12-06

文章信息/Info

Title:

Influences of AmGP on Th17/Treg immune balance in CIA mice

作者:

刘慧; 赵俊云; 杨向竹; 薛慧清; 李彩彩; 马鑫; 张娜; 周然

100029 北京中医药大学基础医学院[刘慧(硕士研究生)、赵俊云、杨向竹、李彩彩(硕士研究生)、马鑫、张娜]; 山西中医学院科研中心(薛慧清、周然)

Author(s):

LIU Hui;  ZHAO Jun-yun;  YANG Xiang-zhu;  et al.

School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China

关键词:

黄芪糖蛋白;  RORγt;  Foxp3;  胶原诱导性关节炎小鼠

Keywords:

Astragalus membranaceus glycoprotein;  RORγt;  Foxp3;  CIA

分类号:

R285

DOI:

10.3969/j.issn.1674-1749.2016.12.004

文献标志码:

-

摘要:

目的 研究16kD黄芪糖蛋白对胶原诱导性关节炎(collagen-induced arthritis,CIA)模型小鼠Th17/Treg细胞免疫平衡的影响,探讨黄芪糖蛋白治疗胶原诱导性关节炎小鼠的免疫作用机理。方法 采用雄性Balb/c小鼠建立牛Ⅱ型胶原诱导性关节炎小鼠模型,将造模成功的小鼠随机分为CIA模型组、氢化可的松阳性对照组、黄芪糖蛋白高、中、低剂量组,另设空白对照组; 各治疗组腹腔注射药物14天,对照组与模型组注射等体积生理盐水。采用流式细胞术检测各组小鼠外周血CD3⁺CD4⁺IL-17A⁺Th17细胞、CD4⁺CD25⁺Foxp3⁺Treg细胞比例; Western blot法检测各组小鼠脾组织中维甲酸相关孤独受体γt(retinoid-related orphan receptor γt, RORγt)和叉头状转录因子3(forkhead box protein 3,Foxp3)的蛋白表达水平。结果 流式检测结果显示,黄芪糖蛋白可显著降低模型组小鼠外周血中CD3⁺CD4⁺IL-17A⁺Th17细胞比例(P＜0.01),提高CD4⁺CD25⁺Foxp3⁺Treg细胞的比例(P＜0.01)。Western blot结果显示黄芪糖蛋白可显著降低模型组小鼠脾组织中RORγt的表达(P＜0.01),提高Foxp3的表达(P＜0.01)。结论 黄芪糖蛋白提高Foxp3的表达水平,降低RORγt的表达水平,恢复Th17/Treg细胞之间的平衡,可能是黄芪糖蛋白治疗胶原诱导性关节炎小鼠的机制之一。

Abstract:

Objective To examine the impacts of 16kD astragalus membranaceus glycoprotein(AmGP)on the balance of Th17/Treg in CIA mice; and to investigate the effects of AmGP on CIA mice and its' mechanism. Methods Bovine type Ⅱcollagen was used to establish CIA model. Mice were randomly divided into five groups: CIA model group, hydrocortisone(HC)positive control group, AmGP groups with low, medium and high dose. In addition, a normal group was built. The HC and AmGP groups were treated by HC or AmGP with intraperitoneal injection, while normal saline was used in the other groups,and the treatment was lasted for 2 weeks. Flow cytometry was used to detect the proportions of CD3⁺CD4⁺IL-17A⁺Th17 and CD4⁺CD25⁺Foxp3⁺Treg cells in peripheral blood of the mice; The protein expressions of RORγt and Foxp3 were evaluated by western blot in mice spleen. Results It is showed that AmGP could significantly reduce the proportion of CD3⁺CD4⁺IL-17A⁺Th17 in peripheral blood of CIA mice(P＜0.01). It could improve the proportion of CD4⁺CD25⁺Foxp3⁺Treg cells(P＜0.01). Western blot results indicated that AmGP could significantly decrease the expression of RORγt (P＜0.01), while increase the expression of Foxp3 in spleen of CIA mice(P＜0.01).Conclusion AmGP recovers the balance of Th17/Treg cells by improving the expressions of Foxp3,and reducing the expression of RORγt. This procedure may be one of the mechanisms for AmGP to cure the CIA mice.

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备注/Memo

备注/Memo:

基金项目: 国家国际科技合作专项(2013DFA30700)
作者简介: 刘慧(1989- ),女,2014级在读硕士研究生。研究方向:16kD黄芪糖蛋白作用于CIA小鼠的免疫作用机理。E-mail:13671081286@163.com
通讯作者: 杨向竹(1970- ),女,博士,副教授,硕士生导师。研究方向:中药的现代物质基础。E-mail:xiangzhuy@126.com

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更新日期/Last Update: 2016-12-06