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[1]秦丽,窦永起,李敏,等.益气活血中药黄芪-丹参对脂多糖致急性肺损伤大鼠防治作用的机制研究[J].环球中医药,2017,10(01):2-6.[doi:10.3969/j.issn.1674-1749.2017.01.001]
　QIN Li,DOU Yongqi,LI Min,et al.Study the mechanism of supplementing qi and activating blood herbs on Lipopolysaccharide-induced acute lung injury in rats[J].,2017,10(01):2-6.[doi:10.3969/j.issn.1674-1749.2017.01.001]
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益气活血中药黄芪-丹参对脂多糖致急性肺损伤大鼠防治作用的机制研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年01期
页码:
2-6
栏目:
论著
出版日期:
2017-01-06

文章信息/Info

Title:
Study the mechanism of supplementing qi and activating blood herbs on Lipopolysaccharide-induced acute lung injury in rats
作者:
秦丽; 窦永起; 李敏; 王毓国
100853 北京,中国人民解放军总医院中医科[秦丽(硕士研究生)、窦永起、李敏、王毓国]
Author(s):
QIN Li;  DOU Yongqi;  LI Min;  et al.
Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing 100853, China
关键词:
益气活血中药;  脂多糖;  急性肺损伤;  Toll样受体4;  白介素1受体相关激酶;  核转录因子κB
Keywords:
Supplementing qi and activating blood herbs;  Lipopolysaccharide;  Acute lung injury;  TLR-4;  IRAK-1;  NF-κB
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.01.001
文献标志码:
A
摘要:
目的 观察益气活血中药黄芪-丹参对脂多糖(lipopolysaccharide, LPS)致急性肺损伤(acute lung injury, ALI)大鼠TLR-4/NF-κB信号通路的影响。方法 SD雄性大鼠40只,随机分成正常对照组、模型对照组、地塞米松组、益气活血中药组,各组10只; 除正常对照组气管内滴注生理盐水,其余各组滴注LPS(5 mg/kg)制备ALI模型; 造模前后各3天干预组予以2 mL相应药物(益气活血中药0.59 g/mL或地塞米松5 mg/kg),其余组予以等量的生理盐水灌胃; 于末次灌胃给药2小时后处死大鼠,免疫组织化学法检测大鼠肺组织中Toll样受体4(Toll-like receptor 4,TLR-4)、白介素1受体相关激酶1(interleukin-1 receptor-associated kinase-1,IRAK-1)、核转录因子κB(Nuclear factor kappa B,NF-κB)蛋白表达的含量,qPCR测定肺组织中TLR-4、IRAK-1、NF-κB mRNA表达量。结果 LPS造模组大鼠肺组织中TLR-4、IRAK-1、NF-κB mRNA表达和蛋白表达均高于正常对照组(P＜0.01),且模型对照组高于地塞米松组和中药组(P＜0.01); 中药组TLR-4、IRAK-1 mRNA表达和蛋白表达量与地塞米松组无明显差异(P＞0.05),而NF-κB mRNA表达和蛋白表达量高于地塞米松组(P＜0.05)。结论 益气活血中药黄芪-丹参对LPS致ALI大鼠的防治作用可能与其下调TLR-4/NF-κB信号通路有关。
Abstract:
Objective To investigate the effects of supplementing qi and activating blood herbs astragalus membranaceus and salvia miltiorrhiza on TLR-4/NF-kappa B signaling pathway in rats with acute lung injury induced by lipopolysaccharide. Methods Forty healthy male SD rats were randomly divided into normal control group, model control group, dexamethasone group, herb group, ten in each group. ALI models were established by intratracheal instillation of LPS(5 mg/kg),while normal control group was instilled normal saline. 3 days before and after making the model,the drug(0.59 g/mL herbs or dexamethasone for 5 mg/kg)or same amount of normal saline was given in each group by gavage. Rats were sacrificed after 2h of the last administration. Then the protein and mRNA expressions of TLR-4, Interleukin-1 receptor-associated kinase-1 and NF-kappa B were measured by reverse transcriptase polymerase chain reaction and immunohistochemistry respectively.Results Compared with normal control group, the protein and mRNA expressions of LPS groups was higher(P＜0.01); compared with model control group, dexamethasone group and herbs group was decreased(P＜0.01); the expression levels of TLR-4, IRAK-1 in herb group were close to dexamethasone group(P＞0.05),but the level of NF-κB was higher in herb group(P＜0.05). Conclusion The protective and therapeutic effects of astragalus membranaceus and salvia miltiorrhiza on LPS-induced ALI in rats maybe due to its ability to modulate TLR-4/ NF-κB signaling pathways.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81303131)
作者简介:秦丽(1990- ),女,2014级在读硕士研究生。研究方向:中西医结合临床。E-mail:qinlizi301@163.com
通信作者:李敏(1980- ),博士,主治医师。研究方向:中西医结合临床。E-mail:kittenlee1115@126.com
更新日期/Last Update: 2017-01-06