|本期目录/Table of Contents|

[1]陈明喆,何伟.橙皮苷对IgA肾病大鼠肾脏组织中TLR-4和NF-κB表达的影响研究[J].环球中医药,2017,10(01):7-11.[doi:10.3969/j.issn.1674-1749.2017.01.002]
　CHEN Mingzhe,HE Wei.Effect of hesperidin on TLR-4 and NF-κB in kidney tissue of IgA nephropathy rats[J].,2017,10(01):7-11.[doi:10.3969/j.issn.1674-1749.2017.01.002]
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橙皮苷对IgA肾病大鼠肾脏组织中TLR-4和NF-κB表达的影响研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年01期
页码:
7-11
栏目:
论著
出版日期:
2017-01-06

文章信息/Info

Title:
Effect of hesperidin on TLR-4 and NF-κB in kidney tissue of IgA nephropathy rats
作者:
陈明喆; 何伟
鄂东医疗集团黄石市中心医院肾内科(陈明喆),老年病科(何伟); 湖北理工学院医学院(何伟)
Author(s):
CHEN Mingzhe;  HE Wei
The Nephrology Department of Huangshi Central Hospital,Huangshi 435000,China
关键词:
橙皮苷;  IgA肾病;  Toll样受体4;  自然细胞杀伤因子-κB
Keywords:
Hesperidin;  IgA nephropathy;  Toll-like receptor 4;  NF-κB
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.01.002
文献标志码:
A
摘要:
目的 研究橙皮苷对IgA 肾病大鼠肾脏组织中TLR-4和NF-κB表达的影响,探讨橙皮苷对肾脏是否有保护作用及其作用机理。方法 雄性SD大鼠60只随机分为正常组、模型组和橙皮苷治疗组,IgA模型建立成功后,橙皮苷治疗组给予橙皮苷10 mg/(kg·d)灌胃治疗,正常组和模型组给予等量生理盐水灌胃,治疗4周后、8周后分别处死大鼠,检测其24小时尿蛋白、血尿素氮、血白蛋白、白介素-1(interleukin-1,IL-1)和白介素-6(IL-6),免疫组化、RT-PCR以及Western blot检测肾组织中TLR-4和NF-κB表达。结果 在整个实验过程中,正常组饮食正常,精神良好,模型组和橙皮苷治疗组在造模成功后表现有一定的食欲不振和精神萎靡,使用橙皮苷治疗后,上述情况有所缓解; 与正常组相比,模型组24小时尿蛋白、血尿素氮、IL-1和IL-6明显升高(P＜0.05),白蛋白明显下降(P＜0.05),治疗4周后,上述指标有明显改善,治疗8周后其基本和治疗4周后一致; RT-PCR、Western blot和免疫组化可见,相较于正常组,模型组TLR-4和NF-κB蛋白及mRNA表达明显增加(P＜0.05),橙皮苷治疗4周后,其有明显降低,治疗8周后,表达更弱(P＜0.05)。结论 橙皮苷可明显下调TLR-4和NF-κB的表达,进而降低炎症反应,改善肾功能,最终保护肾脏组织。
Abstract:
Objective To study the effect of hesperidin on TLR-4 and NF-kB in IgA nephropathy rats,and discuss whether hesperidin have the protective effect to kidney and its protection mechanism. Methods 60 male SD rats were randomly divided into normal group,model group and hesperidin treatment group,after the IgA model was successfully established,hesperidin treatment group was given hesperidin(10 mg/kg·d)to lavage treatment,the normal group and model group was given equal saline lavage,and after 4 weeks and 8 weeks of treatment, rats were sacrificed to detect the 24h urine protein,serum BUN,blood albumin,interleukin 1 and interleukin-6,immunohistochemistry,RT-PCR and Western Blot was used to detect the expression of TLR-4 and NF-κB in renal tissue. Results In the process of the whole experiment,the normal group was fed with normal diet and the spirit was good,model group and the hesperidin group has certain anorexia and depression after molding, the above situation was improved after using hesperidin; Compared with normal group,model group in 24h urinary protein,serum BUN,interleukin-6 and interleukin-1 rise obviously,albumin was significantly lower,after 4 weeks of treatment,those indices were improved significantly,results of 8 weeks and 4 weeks of treatment were basically the same; Compared with normal group,TLR-4 and NF-kB protein and mRNA expression in model group were significant increased,after 4 weeks of treatment of hesperidin, these indices were significantly reduced,after 8 weeks of treatment,the expression was more less(P＜0.05). Conclusion Hesperidin can obviously decrease the expression of TLR-4 and NF-kB and inflammatory reaction,it also can improve renal function,eventually to protect kidney.

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备注/Memo

备注/Memo:
基金项目: 435000 黄石中心医院自筹项目(2014HS023)
作者简介: 陈明喆(1981- ),硕士,主治医师。研究方向:中西医结合治疗老年病。E-mail:12616918@qq.com
通信作者: 何伟(1980- ),硕士,主治医师,讲师。研究方向:中西医结合治疗肾病。E-mail:hsyiyuantougao@163.com
更新日期/Last Update: 2017-01-06