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[1]吕甜甜,王宏亮,邢玮,等.附子对高糖刺激下施万细胞中Krox20-Oct6信号通路及其调控的髓鞘蛋白的影响[J].环球中医药,2017,10(01):12-16.[doi:10.3969/j.issn.1674-1749.2017.01.003]
　LV Tiantian,WANG Hongliang,XING Wei,et al.Aconiti lateralis radix praeparata on the formation of myelin sheath and the Krox20-Oct6 signaling pathway in Schwann cells induced by high glucose[J].,2017,10(01):12-16.[doi:10.3969/j.issn.1674-1749.2017.01.003]
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附子对高糖刺激下施万细胞中Krox20-Oct6信号通路及其调控的髓鞘蛋白的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年01期
页码:
12-16
栏目:
论著
出版日期:
2017-01-06

文章信息/Info

Title:
Aconiti lateralis radix praeparata on the formation of myelin sheath and the Krox20-Oct6 signaling pathway in Schwann cells induced by high glucose
作者:
吕甜甜; 王宏亮; 邢玮; 吴晏; 王伟; 张子剑; 韩静
100029 北京中医药大学基础医学院[吕甜甜(硕士研究生)、王宏亮、邢玮、吴晏、王伟、张子剑、韩静]
Author(s):
LV Tiantian; WANG Hongliang; XING Wei; et al.
Basic Medical College of Beijing University of Chinese Medicine,Beijing 100029,China
关键词:
糖尿病周围神经病变;  附子;  施万细胞;  Krox20-Oct6信号通路
Keywords:
Diabetic peripheral neuropathy(DPN);  Aconiti lateralis radix praeparata;  Schwann cells;  Krox20-Oct6 signaling pathway
分类号:
R932
DOI:
10.3969/j.issn.1674-1749.2017.01.003
文献标志码:
A
摘要:
目的 观察附子对高糖培养下施万细胞中Krox20-Oct6途径及其调控的髓鞘蛋白的影响,以揭示附子治疗糖尿病周围神经病变的机制。方法 将施万细胞分为以下6组:(1)正常组(低浓度葡萄糖);(2)对照组(甘露醇);(3)模型组(高浓度葡萄糖);(4)附子水提物高、中、低剂量组(10 μg/mL,1.0 μg/mL,0.1 μg/mL)。常规培养3天后,采用实时荧光定量PCR法检测各组细胞Oct6、Krox20、髓鞘碱性蛋白和髓鞘蛋白Z mRNA的表达水平,采用Western blot法检测各组细胞的Oct6和Krox20蛋白的表达水平,采用免疫荧光法检测各组细胞的髓鞘碱性蛋白和髓鞘蛋白Z蛋白的表达水平。结果 PCR结果显示,与正常组相比,模型组中Oct6、Krox20、髓鞘碱性蛋白和髓鞘蛋白Z mRNA表达水平下降; 与模型组比,附子水提物各剂量组中Oct6、Krox20、髓鞘碱性蛋白和髓鞘蛋白Z mRNA表达水平上升。Western blot结果证明,与正常组相比,模型组中Oct6蛋白、Krox20蛋白表达水平下降; 与模型组比,附子水提物各剂量组中Oct6蛋白、Krox20蛋白表达水平上升。免疫荧光结果提示,与正常组相比,模型组中髓鞘碱性蛋白蛋白及髓鞘蛋白Z蛋白表达水平下降; 与模型组比,附子水提物各剂量组中髓鞘碱性蛋白蛋白及髓鞘蛋白Z蛋白表达水平上升。结论 高浓度葡萄糖通过抑制Krox20-Oct6途径使施万细胞髓鞘蛋白生成能力下降,而附子可能通过Krox20-Oct6途径促进髓鞘蛋白的形成,从而改善糖尿病周围神经病变。
Abstract:
Objective To investigate the Krox20-Oct6 signaling pathway in Schwann cells induced by high glucose and to analyze the effect of aconiti lateralis radix praeparata on the formation of myelin sheath, and then clarify the mechanisms underlying the therapeutic effect of aconiti lateralis radix praeparata in diabetic peripheral neuropathy. Methods Schwann cell were divided into six groups. In the control group, the cells were supplemented with normal cell culture medium. In the mannitol group, the cells were fed with normal glucose plus mannitol. In the model group, the cells were supplemented with high glucose medium. In the other group, the cells were treated with high glucose medium plus different concentrations of aconiti lateralis radix praeparata(0.1 μg/mL, 1.0 μg/mL and 10.0 μg/mL). After three days, Real-time PCR was used to detect the expression of Oct6, Krox20, myelin basic protein and myelin protein zero mRNA. Western blot was used to detect Oct6 and Krox20 protein expression. Myelin basic protein and myelin protein zero protein expression was detected by immunofluorescence. Results PCR results showed that compared with the control group, the model group showed lower expression of Oct6, Krox20, myelin basic protein and myelin protein zero protein. In comparison to the model group, aconiti lateralis radix praeparata(0.1 μg/mL, 1.0 μg/mL and 10.0 μg/mL)increased the expression of Oct6, Krox20, myelin basic protein and myelin protein zero protein. Western blot results indicated that compared with the control group, the model group showed lower expression of Oct6 and Krox20. In comparison to the model group, aconiti lateralis radix praeparata(0.1 μg/mL, 1.0 μg/mL and 10.0 μg/mL)increased the expression of Oct6 and Krox20. Immunofluorescence results showed that compared with the control group, the model group showed lower expression of myelin basic protein and myelin protein zero protein. In comparison to the model group, aconiti lateralis radix praeparata(0.1 μg/mL, 1.0 μg/mL and 10.0 μg/mL)increased the expression of myelin basic protein and myelin protein zero protein. Conclusion These results indicates that high glucose can decrease the protein of myelin sheath by inhibiting Krox20-Oct6 pathway and that aconiti lateralis radix praeparata improves the diabetic peripheral neuropathy via enhancing the formation of myelin sheath.

参考文献/References:

[1] Dyck PJ, Kratz KM, Karnes JL, et al.The prevalence by staged severity of various types of diabetic neuropathy, retinopathy, and nephropathy in a population-based cohort: the Rochester Diabetic Neuropathy Study[J].Neurology,1993,43:817-824.
[2] Sugimoto K, Murakawa Y, Sima AA.Diabetic neuropathy: a continuing enigma[J].Diabetes/metabolism Research and Reviews,2000,16(6):408-433.
[3] Vinik AI, Park TS,Stansberry KB, et al. Diabetic neuropathies Diabetolojia[J].Diabetoloqia,2000,43(8):957-973.
[4] 张明发.附子温里药理的研究[J].陕西中医,1994,15(2):88-91.
[5] 史瑞锋,初杰.从古代本草文献看附子的应用[J].辽宁中医杂志,2008,35(9):1344-1345.
[6] Han J, Tan P, Li Z, et al.Fuzi Attenuates Diabetic Neuropathy in Rats and Protects Schwann Cells from Apoptosis Induced by High Glucose[J].Plos One,2014,9(1):e86539.
[7] Glenn T D, Talbot W S. Signals regulatingmyelination in peripheral nerves and the Schwann cell response to injury[J].Current Opinion in Neurobiology,2013,23(6):1041-1048.
[8] Svaren J, Meijer D. The molecular machinery of myelin gene transcription in Schwann cells[J].Glia,2008,56(14):1541-1551.
[9] Kawasaki T, Oka N, Tachibana H, et al. Oct6, a transcription factor controlling myelination, is a marker for active nerve regeneration in peripheral neuropathies[J].Acta neuropathologica,2003,105(3):203-208.
[10] Verrier JD, Semple-Rowland S, Madorsky I, et al. Reduction of Dicer impairs Schwann cell differentiation and myelination[J].Neurosci Res,2010,88(12):2558-2568.
[11] Bermingham JR Jr, Scherer SS, O'Connell S, et al. Tst-1/Oct-6/SCIP regulates a unique step in peripheral myelination and is required for normal respiration[J].Genes Dev,1996,10(14):1751-1762.
[12] Topilko P, Schneider Maunoury S, Levi G, et al. Krox-20 controls myelination in the peripheral nervous system[J].Nature,1994,371(6500):796-799.
[13] Zorick T. S, Syroid D. E, Brown A, et al. Krox-20 controls SCIP expression, cell cycle exit and susceptibility to apoptosis in developing myelinating Schwann cells[J].Development(Cambridge, England),1999,126(7):1397-1406.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(30901959); 北京中医药大学自主课题(0100604165)
作者简介: 吕甜甜(1989- ),女,2014级在读硕士研究生。研究方向:糖尿病周围神经及视网膜并发症。E-mail:lvtiantian471398@163.com
通信作者: 韩静(1977- ),女,博士,副研究员。研究方向:糖尿病周围神经及视网膜并发症。E-mail:hanjing8585@163.com
更新日期/Last Update: 2017-01-06