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[1]王宏亮 吕甜甜 邢玮 韩静 吴晏 张子剑 王伟.活血解毒方对糖尿病大鼠视网膜组织中microRNA表达的影响[J].环球中医药,2017,10(03):257-261.[doi:10.3969/j.issn.1674-1749.2017.03.001]
　WANG Hongliang,LV Tiantian,XING Wei,et al.Effect of Huoxue Jiedu recipe on the expressions of microRNA in the retina of diabetic rats[J].,2017,10(03):257-261.[doi:10.3969/j.issn.1674-1749.2017.03.001]
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活血解毒方对糖尿病大鼠视网膜组织中microRNA表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年03期
页码:
257-261
栏目:
论著
出版日期:
2017-03-06

文章信息/Info

Title:
Effect of Huoxue Jiedu recipe on the expressions of microRNA in the retina of diabetic rats
作者:
王宏亮 吕甜甜 邢玮 韩静 吴晏 张子剑 王伟
100029 北京中医药大学基础医学院[王宏亮(硕士研究生)、吕甜甜(硕士研究生)、邢玮(硕士研究生)、韩静、吴晏、张子剑、王伟]
Author(s):
WANG Hongliang;  LV Tiantian;  XING Wei; et al.
College of Basic Medicine,Beijing University of Chinese Medicine,Beijing100029,China
关键词:
糖尿病视网膜病变;  活血解毒方;  MicroRNA
Keywords:
Diabetic retinopathy;  Huoxue jiedu recipe;  MicroRNA
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.03.001
文献标志码:
A
摘要:
目的 观察活血解毒方对糖尿病大鼠视网膜组织中microRNA-125a-3p、microRNA-212-3p、microRNA-290、microRNA-129-5p、microRNA-30c-5p、microRNA-153-3p和microRNA-423-5p表达的影响,探讨其改善糖尿病视网膜病变的机制。方法 选取SPF级雄性SD大鼠15只。一次性腹腔注射链脲佐菌素诱导糖尿病模型大鼠10只,随机分为模型组和活血解毒方组,另设5只为正常对照组。造模成功后第20周开始给药,给药12周后摘取眼球,提取视网膜组织中的总RNA。采用Real-time PCR技术检测microRNA-125a-3p、microRNA-212-3p、microRNA-290、microRNA-129-5p、microRNA-30c-5p、microRNA-153-3p和microRNA-423-5p的表达。结果 与正常对照组相比,模型组microRNA-125a-3p、microRNA-212-3p、microRNA-290、microRNA-129-5p和microRNA-30c-5p表达降低(P＜0.05,P＜0.01,P＜0.05,P＜0.05,P＞0.05),microRNA-153-3p和microRNA-423-5p表达升高(P＜0.01,P＜0.05); 与模型组相比,活血解毒方组microRNA-125a-3p、microRNA-212-3p、microRNA-290、microRNA-129-5p和microRNA-30c-5p表达升高(P＞0.05,P＜0.05,P＞0.05,P＜0.01,P＜0.05),microRNA-153-3p和microRNA-423-5p表达降低(P＜0.01,P＜0.01)。 结论 推测活血解毒方是通过上调糖尿病大鼠视网膜组织中microRNA-125a-3p、microRNA-212-3p、microRNA-290、microRNA-129-5p和microRNA-30c-5p的表达,下调microRNA-153-3p和microRNA-423-5p的表达来起到延缓糖尿病视网膜病变的发生和发展进程的作用。
Abstract:
Objective To observe the effects of Huoxue Jiedu recipe(HJR)on microRNA-125a-3p,microRNA-212-3p,microRNA-290,microRNA-129-5p,microRNA-30c-5p,microRNA-153-3p and micro RNA-423-5pin diabetic rats with retina to explore the mechanism of HJR in improving diabetic retinopathy.Methods 15 SPF male SD rats wereenrolled in this study.Single intraperitoneal injection of streptozotocin(STZ)was used to induce diabetic models.The diabetic rats were randomly divided into model and HJR.The rest 5 normal rats were treated as control group.After 20 weeks,the HJR group given HJR.The rats' retina was removed after 12 weeks of treatment,total RNA was extracted from the retina.Real-time PCR method was used to determine the expressions of microRNA-125a-3p,microRNA-212-3p,microRNA-290, microRNA-129-5p,microRNA-30c-5p,microRNA-153-3p and microRNA-423-5p.Results Compared with the control group,the expressions of microRNA-125a-3p,microRNA-212-3p,microRNA-290,microRNA-129-5p and microRNA-30c-5p in model group were decreased(P＜0.05,P＜0.01,P＜0.05,P＜0.05,P＞0.05),the expressions of microRNA-153-3p and microRNA-423-5p were increased(P＜0.01,P＜0.05).Compared with the model group,the expressions of microRNA-125a-3p,microRNA-212-3p, microRNA-290,microRNA-129-5p and microRNA-30c-5p in HJR group increased(P＞0.05,P＜0.05,P＞0.05,P＜0.01,P＜0.05),the expressions of microRNA-153-3p and microRNA-423-5p were decreased(P＜0.01,P＜0.01).Conclusion HJR may up-regulating the expressions of microRNA-125a-3p,microRNA-212-3p,microRNA-290,microRNA-129-5p,microRNA-30c-5pand down-regulating the expressions of microRNA-153-3p,microRNA-423-5p

参考文献/References:

[1] Micchael TJ,Anistidis V.糖尿病与心血管疾病[M].孙丰雷,主译.2版. 济南:山东科学技术出版社, 2008.
[2] 张俊,周琦,吕红彬. MicroRNA及其在糖尿病视网膜病变中的研究进展[J]. 眼科新进展,2012,32(2):192-195.
[3] Wu X,Shao X,Meng X,et al.Genome-wide analysis of microRNA and mRNA expression signatures in hydroxycamptothecin-resistant gastric cancercells[J]. Acta Pharmacologica Sinica,2011,32(2):259-269.
[4] Mastropasqua R, Toto L, Cipollone F, et al. Role of microRNAs in the modulation of diabeticretinopathy[J]. Progress in retinal and eye research,2014,43:92-107.
[5] 马晓瑭, 潘群文, 何彩霞, 等. MicroRNA125a 对缺血性脑卒中小鼠内皮祖细胞功能的调节作用[J]. 新乡医学院学报,2015,32(8):702-706.
[6] Chen S, Zhao G, Miao H, et al. MicroRNA-494 inhibits the growth and angiogenesis-regulating potential of mesenchymal stem cells[J]. FEBS letters,2015,589(6):710-717.
[7] Murray AR, Chen Q, Takahashi Y, et al. MicroRNA-200b downregulates oxidation resistance 1(Oxr1)expression in the retina of type 1 diabetes model Oxr1 expression in type 1 diabetes model[J]. Investigative ophthalmology & visual science,2013,54(3):1689-1697.
[8] Intine RV, Sarras Jr MPS. Metabolic memory and chronic diabetes complications: potential role for epigeneticmechanisms[J]. Current diabetes reports,2012,12(5):551-559.
[9] 姚青, 韩静, 黄黎明, 等. 活血解毒方对糖尿病早期大鼠视网膜功能的影响[J]. 中国中西医结合杂志,2012,32(9):1271-1274.
[10] 姚青, 韩静, 黄黎明, 等. 活血解毒方对糖尿病大鼠视网膜病变内皮素-1 表达的影响[J]. 中国实验方剂学杂志,2012,18(20):169-172.
[11] 姚青, 韩静, 余俊达, 等. 活血解毒方对糖尿病大鼠视网膜病变的影响[J]. 中国中西医结合杂志,2012,32(3):362-366.
[12] 何洁, 郝改梅, 武志黔, 等. 活血解毒方对糖尿病大鼠视网膜病变的影响研究[J]. 中国全科医学,2015,18(5):507-512.
[13] 郝改梅, 何洁, 韩静, 等. 基于 Ras/Raf-1/ERK 信号转导通路调控 VEGF 的表达探讨活血解毒方对糖尿病大鼠视网膜的影响[J]. 世界中医药,2015,(5):757-761.
[14] Yu J, Liu F, Yin P, et al. Integrating miRNA and mRNA expression profiles in response to heat stress-induced injury in rat smallintestine[J]. Functional & integrative genomics,2011,11(2):203-213.
[15] Che P, Liu J, Shan Z, et al. MicroRNA-125a-5p impairs endothelial cell angiogenesis in aging mice via RTEF-1 downregulation[J]. Aging cell,2014,13(5):926-934.
[16] Ninio-Many L, Grossman H, Shomron N, et al.MicroRNA-125a-3p reduces cell proliferation and migration by targeting Fyn[J]. J Cell Sci,2013,126(13):2867-2876.
[17] Kumarswamy R, Volkmann I, Beermann J, et al. Vascular importance of the miRNA-212/132 cluster[J]. European heart journal,2014,35(45):3224-3231.
[18] Chang HM, Martinez NJ, Thornton JE, et al. Trim71 cooperates with microRNAs to repress Cdkn1a expression and promote embryonic stem cell proliferation[J].Nature communications, 2012,3(2):923.
[19] Tan G, Cao X, Dai Q, et al. A novel role for microRNA-129-5p in inhibiting ovarian cancer cell proliferation and survival via direct suppression of transcriptional co-activators YAP and TAZ[J]. Oncotarget,2015,6(11):8676.
[20] Liu Q, Du GQ, Zhu ZT, et al. Identification of apoptosis-related microRNAs and their target genes in myocardial infarction post-transplantation with skeletal myoblasts[J]. Journal of translational medicine,2015,13(1):270.
[21] 梁春联, 朱华, 黄澜, 等. MicroRNA-153 对靶基因下游信号分子 GSK-3β 表达水平及细胞抗损伤能力的影响[J]. 中国比较医学杂志,2011,21(8):15-19.
[22] Wu LM, Ji JS, Yang Z, et al. Oncogenic role of microRNA-423-5p in hepatocellular carcinoma[J]. Hepatobiliary & Pancreatic Diseases International,2015,14(6):613-618.
[23] 姚青,韩静,黄黎明,等. 活血解毒方对糖尿病大鼠视网膜PEDF表达的影响[J]. 中国实验方剂学杂志,2011,17(23):162-165.

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备注/Memo

备注/Memo:
基金项目: 国家科技重大专项“重大新药创制”专项(2009ZX09103-320)
作者简介: 王宏亮(1989-),女,2014级在读硕士研究生。研究方向:中西医结合药理学研究。E-mail:17801084715@163.com
通信作者: 王伟(1964- ),博士,教授,博士生导师。研究方向:中西医结合药理学研究。E-mail:wangwei26960@126.com
更新日期/Last Update: 2017-03-06