[1]李昕 李雁 刘丽杰 黄瑞音 王谦 孙庆巧 骆长永 万茂婷 梁旭 吴元德 王欣.益气化瘀解毒方对急性呼吸窘迫综合征大鼠NF-κB/p38MAPK通路的影响[J].环球中医药,2017,10(03):275-279.[doi:10.3969/j.issn.1674-1749.2017.03.005]
LI Xin,LI Yan,LIU Lijie,et al.Impact of Yiqi Huayu Jiedu formula on NF-κB/p38MAPK signal pathway in acute respiratory distress syndrome rats[J].,2017,10(03):275-279.[doi:10.3969/j.issn.1674-1749.2017.03.005]
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益气化瘀解毒方对急性呼吸窘迫综合征大鼠NF-κB/p38MAPK通路的影响(
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LI Xin,LI Yan,LIU Lijie,et al.Impact of Yiqi Huayu Jiedu formula on NF-κB/p38MAPK signal pathway in acute respiratory distress syndrome rats[J].,2017,10(03):275-279.[doi:10.3969/j.issn.1674-1749.2017.03.005]
)《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]
- 卷:
- 第10卷
- 期数:
- 2017年03期
- 页码:
- 275-279
- 栏目:
- 论著
- 出版日期:
- 2017-03-06
文章信息/Info
- Title:
- Impact of Yiqi Huayu Jiedu formula on NF-κB/p38MAPK signal pathway in acute respiratory distress syndrome rats
- 作者:
- 李昕 李雁 刘丽杰 黄瑞音 王谦 孙庆巧 骆长永 万茂婷 梁旭 吴元德 王欣
- 100700 北京中医药大学东直门医院教育处(李昕),急诊科[李雁、刘丽杰、孙庆巧(硕士研究生)、骆长永(硕士研究生)、万茂婷(硕士研究生)、梁旭(硕士研究生)]; 北京市朝阳区中医医院内二科(黄瑞音); 北京中医药大学生命科学学院(王谦); 北京协和医学院基础学院(吴元德); 广东骏丰频谱股份有限公司(王欣)
- Author(s):
- LI Xin; LI Yan; LIU Lijie; et al.
- Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
- 关键词:
- 益气化瘀解毒方; 急性呼吸窘迫综合征; 核转录因子-κB; 丝裂原活化蛋白激酶p38
- 分类号:
- R285.5
- 文献标志码:
- A
- 摘要:
- 目的 观察益气化瘀解毒方对脂多糖(lipopolysaccharide, LPS)所致大鼠急性呼吸窘迫综合征(acute respiratory distress syndrome, ARDS)的保护作用并探讨其作用机制。方法 30只SD大鼠随机分为空白组、模型组及益气化瘀解毒方低、中、高剂量组5组,每组6只。尾静脉注射LPS建立ARDS模型。治疗组益气化瘀解毒方灌胃,造模后16小时处死大鼠收集标本,测定大鼠肺系数、肺通透指数、核转录因子-κB p50、IκBα、p38的蛋白表达及光镜下观察肺组织病理学改变。结果 益气化瘀解毒方减少肺损伤大鼠肺泡结构破坏、肺水肿及炎性细胞浸润,降低肺系数(P＜0.05)与肺通透指数(P＜0.01),并在一定程度减少核转录因子-κB p50、丝裂原活化蛋白激酶p38,增加抑制因子IκBα的蛋白表达。结论 益气化瘀解毒方对LPS诱导的ARDS有一定的保护作用,其机制与抑制NF-κB/p38MAPK炎性反应信号通路有关。
- Abstract:
- Objective To observe the impact and mechanism of Yiqi huayu jiedu formula on acute respiratory distress syndrome(ARDS)rats induced by endotoxin. Methods 30 SD rats were randomized control group, model group, YHJ high, middle, low dose group, 6 rats in each group. The ARDS model was established by tail vein injection with lipopolysaccharide. The treatment groups were given corresponding aqueous solution once a day. All groups were sacrificed 16 hours after modeling. Then the lung coefficient, the lung permeability index and the levels of NF-κB p50, IκBα and p38 were measured, and the pathological changes in lung tissues were observed. Results HE staining showed that Yiqi huayu jiedu formula could reduce pulmonary alveolar damage, pulmonary edema and inflammatory cells infiltration. The lung coefficient(P＜0.05)and lung permeability index(P＜0.01)were decreased in treatment groups. The protein expressions of NF-κB p50 and p38 were decreased while IκBα was increased in treatment groups. Conclusion Yiqi huayu jiedu formula had prevention effect on endotoxin induced ARDS. It might relate to the inhibition of NF-κB/p38MAPK inflammatory signal pathway.
参考文献/References:
[1] 秦丽,王毓国,李敏,等.益气活血中药对脂多糖致急性肺损伤大鼠的防治作用[J].环球中医药,2016,9(5):548-552.
[2] 李雁,王双,李昕,等.益气化瘀解毒复方联合超低频电磁场处理水对内毒素诱发大鼠急性肺损伤的影响[J].中医杂志,2015,57(9):783-788.
[3] Abraham E. NF-κB activation[J]. Crit Care Med, 2002,28(4Suppl):100-104.
[4] Moreira-Tabaka H, Peluso J, Vonesch J L, et al. Unlike for human monocytes after LPS activation,release of TNF-α by THP-1 cells is produced by a TACE catalytically different from constitutive TACE[J]. PloS One,2012,7(3):e34184.
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- 备注/Memo:
-
基金项目: 北京中医药大学2015年度校级自主课题(2015-JYB-JSMS059)
作者简介: 李昕(1984- ),女,博士,助理研究员。研究方向:中西医结合防治呼吸系统疾病。E-mail:lxnnn@126.com
通信作者: 李雁(1967- ),女,硕士,主任医师。研究方向:中西医结合防治呼吸系统疾病。E-mail:liyan9921_@126.com
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2017-03-06