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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第10卷

期数:

2017年03期

页码:

279-283

栏目:

论著

出版日期:

2017-03-06

文章信息/Info

Title:

Effects of ursolic acid on immune function of osteosarcoma rats

作者:

陈伟 刘海生 魏萌 王丽红 师艳艳

056001 邯郸市中心医院骨三科(陈伟、魏萌、王丽红、师艳艳); 邯郸市人民医院外一科(刘海生)

Author(s):

CHEN Wei; LIU Haisheng; WEI Meng; et al.

Handan Central Hospital,Handan 056001,China

关键词:

熊果酸;  骨肉瘤;  免疫功能;  机制

Keywords:

Ursolic Acid;  Osteosarcoma cancer;  Immune function;  Mechanism

分类号:

R285.5

DOI:

10.3969/j.issn.1674-1749.2017.03.006

文献标志码:

A

摘要:

目的 研究熊果酸对荷骨肉瘤大鼠免疫功能的影响及其机制。方法 通过于Wistar大鼠股骨远端骨髓腔中注射接种骨肉瘤UMR106细胞的方法建立荷骨肉瘤大鼠模型并腹腔注射给予不同剂量的熊果酸100、50、25 mg/(kg·d)和顺铂2.5 mg/(kg·d)进行干预治疗,疗程5天。观察大鼠生存状态和肿瘤生长状况; 称量瘤体质量并计算抑瘤率,HE染色法观察肿瘤组织形态结构改变,MTT法测定脾淋巴细胞转化率; ELISA法测定血清中白细胞介素-2(interleukin-2,IL-2)和肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)含量; 流式细胞术检测血液中T淋巴细胞亚群CD4⁺、CD8⁺百分率并计算CD4⁺/CD8⁺比值。结果 经熊果酸干预治疗5天荷骨肉瘤大鼠饮食状况好转,瘤体缩小、瘤体质量减轻且硬度较差,呈现肿瘤细胞皱缩、组织片状坏死等变化; 抑瘤率显著升高,脾淋巴细胞转化率显著升高,血清中IL-2、TNF-α含量显著降低,血液中CD4⁺显著升高、CD8⁺百分率显著降低,CD4⁺/CD8⁺比值显著升高,且均具有一定的剂量依赖性。结论 熊果酸具有提高荷骨肉瘤大鼠免疫功能的作用,其机制可能与熊果酸能够有效提高脾淋巴细胞转化率,降低IL-2、TNF-α含量以及提高CD4⁺百分率、降低CD8⁺百分率而提高CD4⁺/CD8⁺比值有关。

Abstract:

Objective To investigate the effects and mechanism of ursolic acid(UA)on immune function of osteosarcoma cancer rats. Methods The osteosarcoma cancer rat models were conducted by injecting osteosarcoma cancer cell line UMR106 and were treated with UA 100,50,25 mg/(kg·d)and Cisplatin 2.5 mg/(kg·d)by intraperitoneal injection for 5 d. The rats' general states and tumor growth conditions were observed,the histopathological changes of tumor tissue were observed by HE staining; he tumor weight was detected and inhibition rate was calculated, the spleen lymphocyte transformation rate was detected by MTT, the content of IL-2 and TNF-α in serum were detected, the percentage of CD4⁺, CD8⁺ in blood were detected and the ratio of CD4⁺/CD8⁺ was calculated. Results The diet situation of the rats in UA treated groups were improved, tumor were reduced and hardness were less,tumor cell showed shrinkage, tissue necrosis, etc; the tumor inhibition rate were increased; the spleen lymphocyte transformation rate were significantly increased; the content of IL-2 and TNF-α in serum were significantly decreased; the CD4⁺ percentage in blood were significantly increased, the CD8⁺ percentage was significantly decreased, the ratio of CD4⁺/CD8⁺ in UA treated groups were significantly increased. The effect of the above mentioned effects of UA has a certain dose dependence. Conclusion UA can effectively improve the immune function of osteosarcoma cancer rat; which perhaps related to its effects of increasing the spleen lymphocyte transformation rate, reducing the content of IL-2 and TNF-α, increasing CD4⁺ percentages,decreasing CD8⁺ percentages and raising the ratio of CD4⁺/CD8⁺.

参考文献/References:

[1] Van DE,Luijtgaarden AC,Kapusta L,et al. High prevalence of late adverse eventsin malignant bone tumour survivors diagnosed at adult age[J]. Neth J Med,2014,72(10):516-522.
[2] Pu F,Chen F,Chen S,et al. Association between GSTP1 polymorphisms and prognosis of osteosarcoma in patients treated with chemotherapy:a meta-analysis[J]. Onco Targets Ther,2015,23(8):1835-1842.
[3] Whelan J,McTieman A,Cooper N,et al. Incidence and survival of malignant bone sarcomas in England 1979-2007[J]. Int J Cancer,2012,131(4):508-517.
[4] Karachaliou N,Pilotto S,Bria E,et al. Platelets and their role in cancer evolution and immune system[J]. Transl Lung Cancer Res,2015,4(6):713-720.
[5] Codony-Servat J,Rosell R. Cancer stem cells and immunoresistance:clinical implications and solutions[J]. Transl Lung Cancer Res,2015,4(6):689-703.
[6] 郝冉冉,王晨静,仲伟珍,等.熊果酸对ox-LDL诱导的人脐静脉内皮细胞氧化损伤的保护作用[J].中药药理与临床,2014,30(4):29-32.
[7] 张明发,沈雅琴.齐墩果酸和熊果酸的抗动脉粥样硬化作用[J].上海医药,2014,35(23):73-79.
[8] 王翔,阎昭,王旭,等.熊果酸纳米脂质体的镇痛作用和抗炎活性[J].山东医药,2013,53(29):31-35.
[9] 刘双,毛汉文,刘佩文,等.熊果酸对高血压大鼠心肌纤维化及p38活化的影响[J].内科急危重症杂志,2016,22(1):64-67.
[10] 方学辉,吴倩,韩雪梅,等.熊果酸抗小鼠H22肝癌移植瘤及对免疫功能的影响[J].肿瘤学杂志,2013,19(3):199-201.
[11] 付伦,朱敏,李招云,等.熊果酸对肺癌细胞株A549及SPCA1细胞周期的抑制作用[J].医学研究杂志,2015,44(5):68-73.
[12] 崔兵兵,程丽芳,侯立静,等.熊果酸抑制人肝癌SMMC-7721细胞生长及凋亡的实验研究[J]. 中国实验方剂学杂志,2013,19(18):181-184.
[13] 李爱霞,赵玉林,张军辉,等.熊果酸诱导人舌鳞状细胞癌Tca8113细胞凋亡的作用[J]. 中国耳鼻咽喉头颈外科,2010,17(9):491-492.
[14] 王忠良,李旭良,张德文,等.Ezrin在UMR106骨肉瘤细胞株移植瘤成瘤及肺转移中的作用[J]. 第三军医大学学报,2009,31(2):156-159.
[15] 于哲,范清宇,张婷. 大鼠骨肉瘤模型的建立[J].动物医学进展,2005,26(2):58-60.
[16] 张杰,吴淑艳,王琳,等. 熊果酸对高脂饮食诱导胰岛素抵抗大鼠脂质代谢的影响[J].中华中医药学刊,2010,28(5):975-978.
[17] 孙志杰,黄静,唐蔚琳,等.枸杞多糖联合氟尿嘧啶对荷瘤小鼠免疫功能影响的实验研究[J].中南药学,2013,11(7):517-520.
[18] 关硕,杨伟,胡俊霞,等. 西黄丸氯仿提取物对荷瘤大鼠免疫清除功能的影响[J].中国现代应用药学,2014,31(2):144-148.
[19] Moore DD,Luu HH. Osteosarcoma[J]. Cancer Treat Res,2014,162:65-92.
[20] 官佳懿,张永红,崔德凤,等. 绿原酸对小鼠脾脏淋巴细胞体外增殖和分泌功能的影响[J].中国农学通报,2013,29(29):27-31.
[21] 吴慧丽,李慧.白芍总苷对溃疡性结肠炎大鼠细胞因子影响的研究[J].中南药学,2010,8(2):128-131.
[22] Wang Z,Wang G,Zhu X,et al. Interleukin-2 is upregulated in patients with a prolapsed lumbar intervertebral disc and modulates cell proliferation,apoptosis and extracellular matrix metabolism of human nucleus pulposus cells[J]. Exp Ther Med,2015,10(6):2437-2443.
[23] Anquetil F,Clavel C,Offer G,et al. IgM and IgA rheumatoid factors purified from rheumatoid arthritis sera boost the Fc receptor and complement-dependent effector functions of the disease-specific anti-citrullinated protein autoantibodies[J]. J Immunol,2015,194(8):3664-3674.
[24] Xie J,Qiu ZF,Han Y,et al. The correlation between HLA-DR or CD38 expression in CD8(+)T cells and CD4(+)T cells count in treatment-naive HIV/AIDS patients[J]. Zhonghua Nei Ke Za Zhi,2016,55(1):36-39.
[25] Liu YL,Wu YB,Zhai K,et al. Determination of Interleukin 27-producing CD4 and CD8 T Cells for The Differentiation Between Tuberculous and Malignant Pleural Effusions[J]. Sci Rep,2016,6:19424-19428.

相似文献/References:

[1]陈伟,刘海生,魏萌,等.熊果酸对人骨肉瘤MG-63细胞增殖和凋亡的影响研究[J].环球中医药,2017,10(04):442.[doi:10.3969/j.issn.1674-1749.2017.04.013]
　CHEN Wei,LIU Haisheng,WEI Meng,et al.Effects of ursolic acid on the proliferation and apoptosis of human osteosarcoma MG-63 cells[J].,2017,10(03):442.[doi:10.3969/j.issn.1674-1749.2017.04.013]

备注/Memo

备注/Memo:

作者简介: 陈伟(1976- ),硕士,主治医师。研究方向:骨科疾病。E-mail:hdsyyzhrf@163.com

常用功能

更新日期/Last Update: 2017-03-06