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[1]谷雨龙 刘斌 姜艳艳.高效液相色谱法测定黄芪赤风汤提取物中萜类和色酮类成分的含量[J].环球中医药,2017,10(03):293-296.[doi:10.3969/j.issn.1674-1749.2017.03.009]
　GU Yulong,LIU Bin,JIANG Yanyan..RP-HPLC analysis of terpenoids and chromones in the extractive of Huangqi Chifeng decoction[J].,2017,10(03):293-296.[doi:10.3969/j.issn.1674-1749.2017.03.009]
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高效液相色谱法测定黄芪赤风汤提取物中萜类和色酮类成分的含量()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年03期
页码:
293-296
栏目:
论著
出版日期:
2017-03-06

文章信息/Info

Title:
RP-HPLC analysis of terpenoids and chromones in the extractive of Huangqi Chifeng decoction
作者:
谷雨龙 刘斌 姜艳艳
100081 北京,中央民族大学中国少数民族传统医学国家民委-教育部重点实验室(谷雨龙); 北京中医药大学中药学院(刘斌、姜艳艳)
Author(s):
GU Yulong; LIU Bin; JIANG Yanyan.
Key Laboratory of Chinese Minority Traditional Medicine(Minzu University of China),State Ethnic Affairs Commission and Ministry of Education,Beijing100081, China
关键词:
高效液相色谱法;  黄芪赤风汤提取物;  芍药苷;  毛蕊异黄酮苷
Keywords:
HPLC;  Extractive of Huangqi chifeng decoction;  Paeoniflorin;  Calycosin-7-glucoside
分类号:
R284.1
DOI:
10.3969/j.issn.1674-1749.2017.03.009
文献标志码:
A
摘要:
目的 建立HPLC法测定黄芪赤风汤提取物中萜类和色酮类成分的含量。方法 采用Venusil MP-C18(4.6×250 mm,5 μm)色谱柱; 以乙腈(A)-水(B)为流动相进行梯度洗脱(0～45 min,10%→18% A; 45～100 min,18%→45% A); 检测波长:220 nm; 流速:1.0 mL/min; 柱温:35 ℃; LCMS-IT-TOF法指认萜类和色酮类成分,分别以芍药苷和毛蕊异黄酮苷为参比计算两类成分含量。结果 芍药苷和毛蕊异黄酮苷的线性范围分别为0.279～6.696 μg和0.192～4.608 μg(r=0.999 8); 平均回收率(n=6)分别为98.83%和98.21%,RSD分别为3.06%和3.24%; 经LCMS-IT-TOF法指认萜类成分4个,色酮类成分12个。结论 所建立的方法快速、简单、准确、专属性强,精密度好,可用于黄芪赤风汤提取物的质量控制。
Abstract:
Objective To develop an HPLC method for determination of terpenoids and chromones in the extractive of Huangqi chifeng decoction. Methods Venusil MP-C18(4.6×250 mm,5 μm)was used to detect. The mobile phase was composed of acetonitrile(A)and water(B)with gradient elution(0～45 min,10%→18% A; 45～100 min,18%→45% A)and the flow rate was 1.0 mL/min. The detection wavelength was 220 nm. The column temperature was 35℃. Terpenoids and chromones were identified by LCMS-IT-TOF method, paeoniflorin and calycosin-7-glucoside was regarded as reference to calculate. Results The linear ranges of paeoniflorin and calycosin-7-glucoside were 0.279～6.696 μg and 0.192 ～ 4.608 μg(r=0.999 8)respectively. The average recovery was 98.83% and 98.21%(RSD＜3.24%,n=6)respectively. Four terpenoids and twelve chromones were identified by LCMS-IT-TOF. Conclusion The method is simple, accurate, repeatable and can be used to control the quality of the extractive of Huangqi chifeng decoction.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81603278); 教育部“长江学者和创新团队发展计划”(IRT_13R63); 中央民族大学青年教师科研专项(2016KYQN51)
作者简介: 谷雨龙(1982- ),博士,实验师。研究方向:传统药物分析。E-mail:guyulong@muc.edu.cn
更新日期/Last Update: 2017-03-06