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[1]张洪梅 史晓飒 刘腾飞 马维维 张艳聪 史新元.不同产地蝉花中腺苷、虫草素和麦角甾醇的含量比较[J].环球中医药,2017,10(03):297-301.[doi:10.3969/j.issn.1674-1749.2017.03.010]
　ZHANG Hongmei,SHI Xiaosa,LIU Tengfei,et al.Comparing research on contents of adenosine, cordycepin and ergosterol in cordyceps cicadae[J].,2017,10(03):297-301.[doi:10.3969/j.issn.1674-1749.2017.03.010]
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不同产地蝉花中腺苷、虫草素和麦角甾醇的含量比较()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年03期
页码:
297-301
栏目:
论著
出版日期:
2017-03-06

文章信息/Info

Title:
Comparing research on contents of adenosine, cordycepin and ergosterol in cordyceps cicadae
作者:
张洪梅 史晓飒 刘腾飞 马维维 张艳聪 史新元
100102 北京中医药大学中药学院[张洪梅(硕士研究生)、史晓飒(硕士研究生)、刘腾飞(博士研究生)、马维维(硕士研究生)、张艳聪(硕士研究生)、史新元]
Author(s):
ZHANG Hongmei; SHI Xiaosa; LIU Tengfei; et al.
School of Chinese Material Medica, Beijing University of Chinese Medicine,Beijing 100102,China
关键词:
蝉花;  腺苷;  虫草素;  麦角甾醇;  高效液相色谱法
Keywords:
Cordyceps cicadae;  Adenosine;  Cordycepin;  Ergosterol;  HPLC
分类号:
R284.1
DOI:
10.3969/j.issn.1674-1749.2017.03.010
文献标志码:
A
摘要:
目的 分析比较10批不同产地蝉花中腺苷、虫草素和麦角甾醇的含量,为蝉花的质量评价及生产用药的筛选提供方法和基础数据。方法 采用高效液相色谱法分别测定不同产地蝉花中腺苷、虫草素和麦角甾醇的含量。结果 腺苷以江苏茅山的蝉花含量最高,为44.957 μg/g,广西玉林的最低,为11.331 μg/g; 虫草素以四川宜宾的含量最高,为191.725 μg/g,江西九江的蝉花含量最低,为25.437 μg/g; 各产地麦角甾醇含量差异相对较小,范围为120.024～301.652 μg/g。结论 不同产地蝉花中的腺苷、虫草素和麦角甾醇的含量差异较大; 该方法适用于蝉花中腺苷、虫草素和麦角甾醇的含量测定,为其质量评价提供参考。
Abstract:
Objective The contents of adenosine、cordycepin and ergosterol in cordyceps cicadae from 10 different areas were compared to provide basis for the quality evaluation and utilization of cordyceps cicadae. Methods The high performance liquid chromatography(HPLC)was used to measure the contents of adenosine、cordycepin and ergosterol in cordyceps cicadae from different areas. Results The highest content of adenosine in cordyceps cicadae was 44.957 μg/g from Jiangsu,and the lowest was 11.331 μg/g from Guangxi. The highest content of cordycepin was from Sichuan and the content was 191.725 μg/g,and that from Jiangsu was 25.437 μg/g,which was the lowest. The content of ergosterol in cordyceps cicadae from different areas varied from 120.024 μg/g to 301.652 μg/g. Conclusion There is difference among the contents of adenosine、cordycepin and ergosterol in Cordyceps cicadae from different areas; the method can be used to determine them in cordyceps cicadae,and to provide the reference for the quality evaluation of cordyceps cicadae.

参考文献/References:

[1] 任颍芳,朱戎.中药蝉花药物考证及其防治肾病的研究进展[J].河南中医,2013,33(5):781-784.
[2] 陈显群,羊悦,杨胜利.中药蝉花菌株筛选及发酵条件优化研究[J].浙江化工,2015,46(2):18-21.
[3] 裘洁,宋捷民.蝉花的药理作用研究进展[J].中国民族民间医药,2009,18(9):4-6.
[4] 宋捷民,陈玲,陈玮,等.蝉花对免疫功能影响的实验研究[J].中国中医药科技,2007,14(1):37-38.
[5] Takano F,Yahagi N,Yahagi R,et al. The liquid culture filtrates of Paecilomycestenuipes(Peck )Samson(=Isaria japonica Yasuda )and Paecilomyces cicadae(Miquel)Sam son(=Isariasinclairii(Berk.)Llond)regulate Th1 and Th2 cytokine response in murine Peyer s' patch cells in vitro and ex vivo[J]. IntImmunopharmacol,2005,5(5):903-916.
[6] Kuo Y C, Weng S C, Chou C J, et al. Activation and proliferation signals in primary human T lymphocytes inhibited by ergosterol peroxide isolated from Cordyceps cicadae[J]. British Journal of Pharmacology, 2003, 140(5):895-906.
[7] 谢炜,郭月芳,盛雨辰.蝉花菌丝体对慢性肾功能衰竭大鼠的治疗作用[J]. 中国医药工业杂志,2011,42(10):770-772.
[8] 刘玉宁,陈以平,王立红,等. 蝉花菌丝抗大鼠肾小管间质纤维化的实验研究[J]. 中国中西医结合肾病杂志,2011,12(3):243-245.
[9] Zhu R,Chen Y P,Deng Y Y,et al. Cordyceps cicadae extracts ameliorate renal malfunction in a remnant kidney model[J]. Journal of Zhejiang Universityence B,2011,12(12):1024-1033.
[10] 徐红娟,莫志宏,余佳文,等.蝉花抗真菌活性成分的分离纯化研究[J].天然产物研究与开发,2010,22(5):794-797.
[11] Sharma S K,Gautam N,Atri N S. Optimized extraction,composition,antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordycepscicadae[J]. Bmc Complementary & Alternative Medicine,2015,15(1):1-10.
[12] 蔡菊芬,姜志明,卢红阳,等.蝉拟青霉不同纯化组分对体外抗肿瘤作用的基础研究[J]. 中华中医药学刊,2010,28(4):760-764.
[13] Wang H,Zhang J,Sit W H,et al. Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells:A proteomic study[J]. Chinese Medicine,2014,9(1):15-15.
[14] Wang J,Zhang D M,Jia J F,et al. Cyclodepsipeptides from the ascocarps and insect-body portions of fungus Cordyceps cicadae[J]. Fitoterapia,2014,97(17):23-27.
[15] 王琪,刘作易.药用真菌蝉花的研究进展[J].中草药,2004,34(4):469-471.
[16] Wang S X,Liu Y,Zhang G Q,et al. Cordysobin,a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordycepssobolifera[J]. Journal of Bioscience & Bioengineering,2012,113(1):42-47.
[17] 温鲁,唐玉玲,张平.蝉花与有关虫草活性成分检测比较[J].江苏中医药,2006,27(1):45-46.
[18] 赵京林.腺苷与心脏保护的研究进展[J].中国心血管病研究杂志,2004,2(8):658-661.
[19] SJ Park,YJ Ahn,SG Lee,et al. Cordycepin:selective growth inhibitor derived from liquid culture of Cordycepsmilitaris against Clostridium spp[J]. Journal of Agricultural and Food Chemistry,2000,48(7):2744-2748.
[20] 韦会平,肖波,胡开治.蛹虫草药用价值考[J].中药材,2004,27(3):215-217
[21] 吕慧慧,杨安伦,张浩. HPLC-DAD同时测定金蝉花中8种核苷类成分的含量[J].实用药物与临床,2015,18(12):1466-1469.
[22] 周尧,滕晔,宋玉良,等.HPLC法测定金蝉花及半野生培养品中腺苷成分的含量[C]//2013第六次临床中药学学术年会暨临床中药学学科建设经验交流会论文集,2013.
[23] 陈安徽,陈宏伟,徐洋,等.蝉花虫草中核苷类成分的分离纯化和鉴定[J]. 食品科学,2013,34(1):131-134.

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备注/Memo

备注/Memo:
作者简介: 张洪梅(1993- ),女,2016级在读硕士研究生。研究方向:中药生物技术研究。E-mail:lovvendy@126.com
通信作者: 史新元(1974- ),女,博士,教授。研究方向:中药生物技术研究。E-mail:xyshi@126.com
更新日期/Last Update: 2017-03-06