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[1]穆凌云,李金霞,张秋云,等.截断逆挽方对慢加急性肝衰竭大鼠死亡受体途径的影响[J].环球中医药,2017,10(04):410-415.[doi:10.3969/j.issn.1674-1749.2017.04.007]
　MU lingyun,LI jinxia,ZHANG qiuyun,et al.Effect of JieDuan NiWan prescription on death receptor pathway in acute-on-chronic liver failure rat model[J].,2017,10(04):410-415.[doi:10.3969/j.issn.1674-1749.2017.04.007]
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截断逆挽方对慢加急性肝衰竭大鼠死亡受体途径的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年04期
页码:
410-415
栏目:
论著
出版日期:
2017-04-06

文章信息/Info

Title:
Effect of JieDuan NiWan prescription on death receptor pathway in acute-on-chronic liver failure rat model
作者:
穆凌云; 李金霞; 张秋云; 陈煜; 高连印; 杜宇琼
100069 北京, 首都医科大学中医药学院[ 穆凌云(硕士研究生)、李金霞(硕士研究生)、张秋云、高连印、杜宇琼]; 中医络病研究北京市重点实验室[ 穆凌云( 硕士研究生)、张秋云、高连印、杜宇琼]; 首都医科大学附属佑安医院人工肝中心(陈煜)
Author(s):
MU lingyun;  LI jinxia;  ZHANG qiuyun;  et al.
Capital Medical University School of Traditional Chinese Medicine & Beijing Key laboratory of TCM Collateral Disease Theory Research, Beijing 100069, China
关键词:
慢加急性肝衰竭;  截断逆挽方;  细胞凋亡;  死亡受体途径
Keywords:
Acute-on-Chronic Liver Failure;  Jieduan Niwan formula;  Apoptosis;  Death receptor pathways
分类号:
R259
DOI:
10.3969/j.issn.1674-1749.2017.04.007
文献标志码:
A
摘要:
目的 观察“截断逆挽方”对慢加急性肝衰竭大鼠JNK信号通路死亡受体途径的影响。方法 SPF级Wistar雄性大鼠70只,随机分为正常组、模型组、截断逆挽方组和SP600125组,各组大鼠给予猪血清腹腔注射13周,联合D氨基半乳糖/脂多糖(D-galactosamine/lipopolysaccharide,D-GalN/LPS)急性攻击,构建慢加急性肝衰竭大鼠模型,截断逆挽方组在急性攻击前给予截断逆挽方连续灌胃3天,SP600125组于急性攻击前半小时腹腔注射。各组大鼠均在急性攻击后4、8、12小时平行取材。用免疫组化法(Immunohistochemical detection,IHC)检测肝组织中半胱氨酸蛋白酶-8(Cysteine Proteinase-8,Caspase-8)、Caspase-3、脂肪酸合成酶(Fatty acid synthase,Fas)的表达量,用蛋白印迹法(Western Blot,WB)检测肝组织中Fas配体(Fas ligand,FasL)的表达量。结果 与正常组相比,模型组各时间点Caspase-3、Caspase-8、Fas、FasL表达增多,差异有统计学意义(P＜0.05); 与模型组相比,截断逆挽方组及SP600125组在4小时和8小时表达减少,差异有统计学意义(P＜0.01或P＜0.05),12小时较模型组升高,差异无统计学意义(P＞0.05)。结论 截断逆挽方可以有效降低Caspase-3、Caspase-8、Fas、FasL的表达量,阻断死亡受体途径,减少模型大鼠肝细胞凋亡。
Abstract:
Objective To observe the effect of Jieduan Niwan(JDNW) on the death receptor pathway of JNK signaling pathways in acute-on-chronic liver failure(ACLF)rats, and study its mechanism. Methods 70 SPF male Wistar rats were randomly divided into normal group, model group, Jieduan-Niwan prescription group and JNK inhibitor SP600125 group. Except normal group, other groups were given intraperitoneal injection with porcine serum for 13 weeks combined with D-galactosamine / lipopolysaccharide(D-GalN/LPS)acute attack to induce the model of ACLF, JDNW group intragastric administration for 3 days before acute attack, SP600125 group was intraperitoneal injection half an hour before acute attack. The rats were sacrificed in 4,8,12h after acute attack. Caspase-3, Caspase-8, Fas in liver tissue was detected by immunohistochemical method, the expression of FasL in liver tissue was detected by Western Blot. Results Compared with normal group, the expression of Caspase-3, Caspase-8, Fas, FasL were increased(P＜ 0.05)in model group at different time points; compared with the model group, JDNW group and SP600125 group was reduced at 4h and 8h(P＜0.01 or P＜0.05), while that was higher than model group at 12h, result had not statistically significant(P＞0.05). Both JDNW group and SP600125 group can reduce the expression of Caspase-3, Caspase-8, Fas, FasL, and the mechanism was similar. Conclusion The prescription of Jieduan-Niwan can effectively reduce the expression of Caspase-3, caspase-8, Fas, FasL, the mechanism may be related to blocking the death receptor pathway of JNK signaling pathway.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 首都中医药研究专项(14ZY01)
作者简介: 穆凌云(1992- ),女,2014级在读硕士研究生。研究方向:中医药防治肝胆证治规律。E-mail:mumu920308@163.com
通信作者: 张秋云(1962- ),女,博士,教授,博士生导师。研究方向:中医内科肝病。E-mail: zhangqiuyun8202@aliyun.com
更新日期/Last Update: 2017-04-06