|本期目录/Table of Contents|

[1]乔天阳,刘文兰,王文娟,等.一贯煎及骨髓间充质干细胞对肝纤维化大鼠阴虚证表征影响的实验研究[J].环球中医药,2017,10(04):419-424.[doi:10.3969/j.issn.1674-1749.2017.04.009]
　QIAO Tianyang,LIU Wenlan,WANG Wenjuan,et al.Experimental study on the effect of Yiguanjian and bone marrow mesenchymal stem cells on the expression of yin deficiency syndrome in rats with hepatic fibrosis[J].,2017,10(04):419-424.[doi:10.3969/j.issn.1674-1749.2017.04.009]
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一贯煎及骨髓间充质干细胞对肝纤维化大鼠阴虚证表征影响的实验研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年04期
页码:
419-424
栏目:
论著
出版日期:
2017-04-06

文章信息/Info

Title:
Experimental study on the effect of Yiguanjian and bone marrow mesenchymal stem cells on the expression of yin deficiency syndrome in rats with hepatic fibrosis
作者:
乔天阳; 刘文兰; 王文娟; 王程; 孟月
100069 北京,首都医科大学中医药学院[乔天阳(硕士研究生)、刘文兰、王文娟、孟月(硕士研究生)、王程(硕士研究生)]
Author(s):
QIAO Tianyang; LIU Wenlan; WANG Wenjuan; et al.
School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China
关键词:
阴虚证;  肝纤维化;  一贯煎;  骨髓间充质干细胞
Keywords:
Yin deficiency syndrome;  Liver fibrosis;  Yiguanjian;  Bone marrow mesenchymal stem cells
分类号:
R259
DOI:
10.3969/j.issn.1674-1749.2017.04.009
文献标志码:
A
摘要:
目的 观察骨髓间充质干细胞在一贯煎的作用下对肝纤维化大鼠阴虚证的影响并探讨该过程的发生机制。方法 将54只SD大鼠随机分为6组,即正常对照组、模型组、阳性对照组、骨髓干细胞组、骨髓干细胞一贯煎组及一贯煎治疗组。除正常对照组外,其他5组建立CCl4诱导的肝纤维化模型。造模停止第三天,骨髓干细胞组及骨髓干细胞一贯煎组经尾静脉注射骨髓间充质干细胞。一贯煎组及骨髓干细胞一贯煎组给予一贯煎浓缩液灌胃,模型组、正常组灌服生理盐水,阳性组给予秋水仙碱灌胃,每天一次,一共给药4周,给药期间每周测量大鼠体质量、体温、血流速度、饮水量。4周后取材,将pH试纸置于大鼠舌面20秒,测量前后重量变化,判定大鼠舌面干湿度; 通过HE染色和马松染色评价肝脏炎症活动度和纤维化程度; 通过苦味酸—天狼星红染色评判造模大鼠肝组织细胞胶原面积比; 通过荧光示踪评判骨髓间充质干细胞在肝纤维化大鼠肝组织中的表达。结果(1)一贯煎加骨髓间充质干细胞组的炎症活动度和纤维化程度明显低于模型组,差异有统计学意义(P＜0.05);(2)肝纤维化大鼠有阴虚证的表征,一贯煎和骨髓间充质干细胞可以改善大鼠阴虚证的症状。(3)中药组、骨髓间充质干细胞组、中药加骨髓间充质干细胞组和阳性组体重增长均明显高于模型组(P＜0.05); 一贯煎加骨髓间充质干细胞组大鼠舌重较骨髓间充质干细胞组较模型组增高(P＜0.05); 模型组舌面温度为较正常组明显增高(P＜0.05); 一贯煎加骨髓间充质干细胞组与模型组相比较明显降低(P＜0.05); 一贯煎加骨髓间充质干细胞组舌血流速度与模型组相比较降低更为明显(P＜0.05); 一贯煎加骨髓间充质干细胞组大鼠饮水量与模型组相比较明显降低(P＜0.05)。(4)一贯煎组、阳性对照组、骨髓间充质干细胞组和一贯煎加骨髓间充质干细胞组的胶原面积比明显低于模型组(P＜0.05)。(5)一贯煎加骨髓间充质干细胞组的骨髓间充质干细胞荧光面积与骨髓间充质干细胞组相比较明显增高(P＜0.05)。结论 骨髓间充质干细胞可以改善肝纤维化大鼠的阴虚证表征,而一贯煎通过促进骨髓间充质干细胞向肝组织转移而增强骨髓间充质干细胞对肝纤维化大鼠阴虚证表征的改善作用。
Abstract:
Objective To observe the effect of Yiguanjian on bone marrow mesenchymal stem cells on the yin deficiency syndrome in rats with liver fibrosis, and to explore the mechanism of the process.Methods 54 male Sprague-Dawlay rats were divided into normal group, model group, positive group, bone marrow mesenchymal stem cells group, Yiguanjian and bone marrow mesenchymal stem cells group and Yiguanjian group. In addition to the normal group, hepatic fibrosis model was induced by CCl4. After third day of modeling, bone marrow stem cells group and Yiguanjian and bone marrow stem cells group was injected with bone marrow mesenchymal stem cells. Yiguanjian group and Yiguanjian and bone marrow stem cells group was intragastric administration with Yiguanjian(2 mL/200 g),1 time/d. Model group and normal group was intragastric administration with normal saline. Positive group was intragastric administration with colchicine. The weight changes before and after was measured to determine the humidity of rat tongue. The body weight, temperature, amount of blood flow velocity, water intake was measured of rats weekly. After 4 weeks, the pH test strips was put at the rat tongue for 20 seconds; HE staining staining was used to evaluate the activity of liver inflammation and the degree of fibrosis; picric acid - Sirius red staining was used to evaluate area ratio of liver tissue collagen; fluorescence tracer was used to evaluate the expression of bone marrow mesenchymal stem cells in rat liver tissue. Results(1)Compared with the model group, the inflammatory activity and the degree of fibrosis was significantly lower than the Yiguanjian and bone marrow mesenchymal stem cells group. The difference was statistically significant(P＜0.05).(2)The liver fibrosis rats had characterization of yin deficiency syndrome, Yiguanjian and bone marrow mesenchymal stem cells can improve the symptoms of yin deficiency syndrome.(3)Weight gain of Yiguanjian group, bone marrow mesenchymal stem cells group, Yiguanjian combined with bone marrow mesenchymal stem cells group and positive group was significantly higher than that of model group(P＜0.05); Yiguanjian combined with bone marrow mesenchymal stem cells in rats with tongue bone marrow mesenchymal stem cells group was higher than that of model group(P＜0.05); the temperature on the surface of tongue of model group was significantly higher than the normal group(P＜0.05). Compared with the model group, the Yiguanjian and bone marrow mesenchymal stem cells(MSCs)were significantly lower(P＜0.05); blood flow velocity of tongue of Yiguanjian and bone marrow mesenchymal stem cells group was decreased significantly compared with the model group(P＜0.05); water intake of Yiguanjian and bone marrow mesenchymal stem cells group was significantly decreased compared with the model group(P＜0.05).(4)Area ratio of liver tissue collagen of Yiguanjian group, positive control group, the bone marrow mesenchymal stem cells group and Yiguanjian and bone marrow mesenchymal stem cells groups was significantly lower than that of the model group(P＜0.05).(5)Fluorescent area of mesenchymal stem cells of Yiguanjian and bone marrow mesenchymal stem cells group was increased significantly compared with bone marrow mesenchymal stem cells group(P＜0.05). Conclusion Bone marrow mesenchymal stem cells can improve the characteristic of yin deficiency syndrome of liver fibrosis in rats, and Yiguanjian can promote bone marrow mesenchymal stem cells transfer into liver to improve the syndrome of yin deficiency in rats with hepatic fibrosis

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81573879); 首都中医药研究专项重点课题(15ZY03)
作者简介: 乔天阳(1991- ),女,2014级在读硕士研究生。研究方向:肝纤维化中医证候病理的研究。E-mail:qiao.tianyang@163.com
通信作者: 刘文兰(1968- ),女,博士,副教授,硕士生导师。研究方向:慢性肝炎中医证候病理基础的研究。E-mail:wenlanliu1900@126.com
更新日期/Last Update: 2017-04-06