|本期目录/Table of Contents|

[1]石玥,穆国华,吴丽婷,等.二补助育改良方对小鼠子宫内膜胞饮突、LIF、ER和PR表达的影响[J].环球中医药,2017,10(04):425-429.[doi:10.3969/j.issn.1674-1749.2017.04.010]
　SHI Yue,MU Guohua,WU Liting,et al.Effect of Erbu Zhuyu improving prescription on pinopodes, LIF, ER and PR expression in mice endometrium[J].,2017,10(04):425-429.[doi:10.3969/j.issn.1674-1749.2017.04.010]
点击复制

二补助育改良方对小鼠子宫内膜胞饮突、LIF、ER和PR表达的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年04期
页码:
425-429
栏目:
论著
出版日期:
2017-04-06

文章信息/Info

Title:
Effect of Erbu Zhuyu improving prescription on pinopodes, LIF, ER and PR expression in mice endometrium
作者:
石玥; 穆国华; 吴丽婷; 申萌萌; 赵青竹; 刘雁峰
100700 北京中医药大学东直门医院妇科[石玥(博士研究生)、穆国华(硕士研究生)、吴丽婷(硕士研究生)、申萌萌(博士研究生)、赵青竹(硕士研究生)、刘雁峰]
Author(s):
SHI Yue;  MU Guohua; WU Liting; et al.
Department of Ggynaecology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
关键词:
二补助育改良方;  子宫内膜容受性;  胞饮突;  白血病抑制因子;  雌激素受体;  孕激素受体
Keywords:
Erbu Zhuyu improving prescription;  Endometrial receptivity;  Pinopodes;  Leukemia inhibitory factor;  Estrogen receptor;  Progesterone receptor
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.04.010
文献标志码:
A
摘要:
目的 观察二补助育改良方对胚泡着床障碍模型小鼠子宫内膜胞饮突、白血病抑制因子(leukemia inhibitory factor,LIF)、雌激素受体(estrogen receptor,ER)和孕激素受体(progesterone receptor,PR)表达的影响。 方法 建立小鼠胚泡着床障碍模型,将50只实验动物分为5组:空白组、模型组、西药组1(补佳乐组)、西药组2(阿司匹林组)、二补助育改良方组,每组10只。造模与给药同时进行,其后处死小鼠,扫描电镜观察胞饮突的表达,并采用免疫组化HBP法检测小鼠子宫内膜LIF、ER、PR的表达量。 结果 扫描电镜下,二补助育改良方组小鼠子宫内膜表面形态较规整,大量微绒毛及丰富胞饮突表达,细胞之间的间隙明显。免疫组化结果示,二补助育改良方组小鼠子宫内膜LIF、ER、PR表达较模型组明显增加(P＜0.05); LIF表达低于补佳乐组(P＜0.05),ER表达优于补佳乐组(P＜0.05),PR表达与补佳乐组无明显差异(P＞0.05); 各指标与阿司匹林组比较无明显差异(P＞0.05)。 结论 二补助育改良方可能通过调节胚泡着床期子宫内膜胞饮突、LIF、ER和PR的表达,从而提高子宫内膜容受性,参与胚泡植入的黏附过程,改善着床障碍,创造良好的受孕环境,促进胚泡着床。
Abstract:
Objective To observe the effect of Erbu Zhuyu improving prescription on pinopodes, LIF, ER and PR expression in endometrium of mice model with dysfunctional embryo implantation. Methods 50 experimental animals were used to establish model of dysfunctional embryo implantation, and the mice were divided into five groups(10 in each group): blank group, model group, western medicine treated group 1(progynova group), western medicine treated group 2(aspirin group), Erbu Zhuyu improving prescription group. Pinopodes in uterine endometrium were examined by scanning electron microscopy. LIF, ER and PR expressions were detected by immunohistochemistry. Results Scanning electron microscopy showed that the endometrium of Erbu Zhuyu improving prescription group was more regular and the expression of pinopodes were abundant, and the space between the cells was obvious. Immunohistochemical results showed LIF, ER and PR expression of Erbu Zhuyu improving prescription group were obviously higher than model group(P＜0.05). Compared with the progynova group, LIF level of Erbu Zhuyu improving prescription was lower(P＜0.05)while ER level was higher, and PR level was no obvious difference(P＞0.05), all above index of Erbu Zhuyu improving prescription was equal to the aspirin group. Conclusion Erbu Zhuyu improving prescription might regulate the expression of pinopodesand, LIF, ER and PR expression of blastocyst implantation period, improve the endometrial receptivity and contribute to the adhesion of embryo implantation, and to improve and implantation dysfunction, to create a good environment to promote pregnancy and embryo implantation.

参考文献/References:

[1] 王铁枫,刘雁峰,郭锐利.中医药对子宫内膜容受性作用的研究进展[J].北京中医药大学学报(临床版),2010,17(4):34-36.
[2] 刘雁峰,江媚,孙天琳,等.二补助育汤对子宫内膜容受性影响的临床研究[J].世界中医药,2012,7(3):195-197.
[3] 江媚,刘雁峰,黄羚,等. 基于小鼠胚泡着床障碍模型探讨二补助育汤对子宫内膜SCF mRNA表达的影响[J]. 中华中医药杂志,2014,(5):1583-1586.
[4] 冯苗,李素春.子宫内膜着床窗期血管生成状态及胞饮突表达对胚胎着床的影响[J].中国实用妇科与产科杂志,2010,26(7):525-529.
[5] AghajanovaL,Stavreus-Evers A, NikasY,et al. Coexpression of pinopodes and leukemia inhibitory factor, as well as its receptor,in human endometrium[J]. Fertility and sterility, 2003, 79(suppl 1): 808-814.
[6] Salleh N, Giribabu N.Leukemia inhibitory factor: roles in embryo implantation and in nonhormonal contraception[J]. Scientific World Journal, 2014: 201514.
[7] Chen QJ, Sun XX, Li L, et al. Effects of ovarian stimulation on endometrial integrin beta3 and leukemia inhibitory factor expression in the peri-implantation phase[J]. Fertil&Steril,2008, 89(5 suppl): 1357-1363.
[8] Mitchell M H,Swanson R J,Oehninger S.In vivo effect of leukemia inhibitory factor(LIF)and an anti-LIF polyclonal antibody on murine embryo and fetal development followingexposure at the time of transcervical blastocyst transfer[J]. Biology of reproduction, 2002,67(2): 460-464.
[9] Sherwin J R A, Freeman T C,Stephens R J,et al. Identification of genes regulated byleukemia-inhibitory factor in the mouse uterus at the time of implantation[J]. MolecularEndocrinology,2004,18(9): 2185-2195.
[10] Chen Q,SunX,Li L,et al. Effects of ovarian stimulation on endometrial integrin(33 andleukemia inhibitory factor expression in the peri-implantation phase[J]. Fertility and sterility,2008,89(5): 1357-1363.
[11] Thomas K, Thomson A, Wood S,et al. Endometrial integrin expression in womenundergoing in vitro fertilization and the association with subsequent treatment outcome[J].Fertility and sterility, 2003, 80(3): 502-507.
[12] 王玉真,李美芝.分子标记与胚泡着床[J].国外医学:妇产科学分册,2003,30(5):293-294.
[13] Orla MC, Biserka MJ,John PL,et al. Reproductive function of the progesterone receptor isoforms: lessons from knock—out mice. Mol Cel Endocrinol,2001,179(3):97-103.
[14] Bagehi IC,Kumar5.Stood-regulated molecular markers of implantation[J].Semin Reprod Endoerinol,1999,17(3):235-240.
[15] 肖承悰,刘雁峰,江媚. “补肾气,通胞脉”改善子宫内膜容受性[J]. 生殖与避孕,2014,(7):595-598.
[16] 王素霞,赵红丽,孙玉英. 安胎合剂对GnRHa超排卵小鼠子宫内膜容受性的影响[J]. 中华中医药学刊,2009, 27(2):344-346.
[17] AghajanovaL.Leukemia inhibitory factor and human embryo implantation[J].Ann NY ScadSci,2004,103(4):176-183.

相似文献/References:

[1]尹晓丹 何军琴.补肾活血方对多囊卵巢综合征不孕症患者子宫内膜容受性及雌二醇、孕酮影响[J].环球中医药,2015,8(06):672.[doi: A doi:10.3969/j.issn.1674-1749.2015.06.008]
　YIN Xiao-dan,HE Jun-qin..Effect of reinforcing kidney and activating blood decoction on endometrial receptivity and estrogen, progesterone of infertility patients with polycystic ovary syndrome[J].,2015,8(04):672.[doi: A doi:10.3969/j.issn.1674-1749.2015.06.008]

备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81273789,81473721); 国家中医药管理局全国名老中医肖承悰教授学术经验研究及传承工作室; 2014年北京中医药大学薪火传承项目
作者简介: 石玥(1988- ),女,2014级在读博士研究生,医师。研究方向:女性不孕症的中医药防治。E-mail:october5845@163.com
通信作者: 刘雁峰(1963- ),博士,主任医师,博士生导师。研究方向:女性不孕症及更年期综合征的中医药防治。E-mail:liuyaf8888@sina.
更新日期/Last Update: 2017-04-06