|本期目录/Table of Contents|

[1]邹德辉,卢宗孝,晏珺,等.电针“委中”对大鼠腰多裂肌损伤后LIF、IL-17表达的影响[J].环球中医药,2017,10(04):436-442.[doi:10.3969/j.issn.1674-1749.2017.04.012]
　ZOU Dehui,LU Zongxiao,YAN Jun,et al.Effects of electroacupuncture “Weizhong”(BL 40)on the expression of LIF and IL-17 in rats with multifidus muscle injury[J].,2017,10(04):436-442.[doi:10.3969/j.issn.1674-1749.2017.04.012]
点击复制

电针“委中”对大鼠腰多裂肌损伤后LIF、IL-17表达的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年04期
页码:
436-442
栏目:
论著
出版日期:
2017-04-06

文章信息/Info

Title:
Effects of electroacupuncture “Weizhong”(BL 40)on the expression of LIF and IL-17 in rats with multifidus muscle injury
作者:
邹德辉; 卢宗孝; 晏珺; 陈玉佩; 刘通; 陈冬荔; 张佳怡; 许玥; 白玉琢; 张莉; 霍则军
100029 北京中医药大学针灸推拿学院[邹德辉(硕士研究生)、卢宗孝(硕士研究生)、晏珺(硕士研究生)、陈冬荔(硕士研究生)、张佳怡(硕士研究生)、许玥(硕士研究生)、陈玉佩(博士研究生)、白玉琢(博士研究生)、张莉]; 广东省第二中医医院(刘通); 北京大学第三医院中医科(霍则军)
Author(s):
ZOU Dehui; LU Zongxiao; YAN Jun; et al.
Acupuncture-Moxibustion and Tuina,Beijing University of Traditional Chinese Medicine,Beijing 100029,China
关键词:
多裂肌;  损伤修复;  炎症因子;  白血病抑制因子;  白介素17
Keywords:
Multifidus muscle;  Damage respair;  Inflammatory factor;  Leukaemia inhibitory factor;  Interleukin-17
分类号:
R245
DOI:
10.3969/j.issn.1674-1749.2017.04.012
文献标志码:
A
摘要:
目的 观察电针“委中”对大鼠腰多裂肌损伤后组织形态学变化以及对骨骼肌肌肉因子白血病抑制因子(leukemia inhibitory factor,LIF)、骨骼肌损伤前炎症因子白介素17(interleukin,IL-17)表达含量的影响。 方法 将90只雄性SD大鼠随机分为空白组、模型组、模型对照组、电针委中组、电针肾俞组,每组18只。每组再随机分为1天、3天、7天三个时间点。麻醉后于双侧L4-5多裂肌注射0.5%布比卡因溶液复制多裂肌损伤模型,通过光镜观察分析造模后多裂肌形态结构的变化。通过ELISA和免疫组化观察1天、3天、7天多裂肌LIF、IL-17表达含量的动态时相变化。 结果 造模后,与空白组比较,模型组不同时间点的LIF及IL-17表达含量均明显增加(P＜0.01); 与模型组比较,电针委中和电针肾俞组的IL-17表达含量有所降低(P＜0.01),而LIF的表达含量有所增加(P＜0.01); 与电针肾俞组比较,电针委中组在1天后下调IL-17的表达含量更明显(P＜0.05),在3天、7天后上调LIF的作用更显著(P＜0.05)。结论 电针委中可促进IL-17的表达下调,减轻炎症反应的程度,同时可增加LIF的表达含量,促进骨骼肌中葡萄糖的摄取,利于骨骼肌修复。
Abstract:
Objective To observe the effect of electroacupuncture(EA)stimulate at “Weizhong”(BL 40)on the expression of leukaemia inhibi-tory factor(LIF)and interleukin-17(IL-17)in rats with multifidus muscle injury.Methods A total of 90 rats were randomly divided into blank group, model control group, model group, EA “Weizhong”(BL 40)group and a EA “Shenshu”(BL 23)group, 18 rats in each group. Each group was randomly divided into a 1-day subgroup, a 3-day subgroup and a 7-day subgroup again, 6 rats in each subgroup. Both sides of multifidus muscle(L4 and L5)were injected with 0.5% bupivacaine. The morphological and ultrastructural changes of multifidus muscle were observed and analyzed with light microscope at 1st, 3rd, 7th day after model establishment. The expression of leukaemia inhibi-tory factor(LIF)and interleukin-17(IL-17)was measured by immunohistochemical method and elisa method.Results After modeling, compared with the blank group, the expression of LIF and IL-17 in multifidus muscle of model group was significantly increased(P＜0.01); Compared with the model group, the expression of the LIF were significantly increased(P＜0.01)and the expression of IL-17 in multifidus muscle was significantly decreased(P＜0.01)in the EA “Weizhong” group and EA “Shenshu” group. Compared with EA “Shenshu” group, the expression of the IL-17 in multifidus muscle was significantly decreased at 1th day; the expression of LIF was significantly increased at 3th day and 7th day. Conclusion EA “Weizhong”(BL 40)can increase the expression of LIF and decrease the expression of IL-17, and can reduce inflammatory reaction and promote skeletal muscle glucose uptake, so as to be beneficial to skeletal muscle repair.

参考文献/References:

[1] Wagner H, Anders C, Puta C,et al.Musculoskeletal support of lumbar spine stability[J].Pathophysiology:the official journal of the International Society for Pathophysiology/ISP,2005,12(4):257-265.
[2] Tumer NJ,Badylak SF.Regeneration of skeletal muscle[J].Cell and Tissue Research,2012,347(3):759-774.
[3] Henningsen J, Rigbolt KT, Blagoev B, et al. Dynamics of the skeletal muscle secretome during myoblast differentiation[J]. Mol Cell Proteomics, 2010, 9(11): 2482-2496.
[4] Stranska Z, Svacina S. Myokines-muscle tissuehormones[J]. Vnitrni lekarstvi, 2015, 61(4): 365-368.
[5] Pedersen BK, Steensberg A, Fischer C, et al.Searching for the exercise factor: is IL-6 a candidate?[J]. J Muscle Res Cell Motil, 2003, 24(2): 113-119.
[6] Pedersen BK, Febbraio MA. Muscles, exercise and obesity: skeletal muscle as a secretoryorgan[J]. Nat Rev Endocrinol, 2012, 8(8): 457-465.
[7] Pedersen BK. Muscles and theirmyokines[J]. J Exp Biol, 2011, 214(Pt 2): 337-346.
[8] Pedersen BK. Muscle as a secretoryorgan[J]. Compr Physiol, 2013, 3(3): 1337-1362.
[9] Pratesi A,Tarantini F,Di Bari M.Skeletal muscle:an endocrineorgan[J]. Clin Cases Miner Bone Metab, 2013, 10(1): 11-14.
[10] Stranska Z, Svacina S. Myokines-muscle tissuehormones[J]. Vnitrni lekarstvi, 2015, 61(4): 365-368.
[11] Broholm C, Laye MJ, Brandt C, et al. LIF is a contraction-induced myokine stimulatinghuman myocyte proliferation[J]. J Appl Physiol, 2011, 111(1): 251-259.
[12] Kurek JB, Bower JJ, Romanella M, et al. The role of leukemia inhibitory factor in skeletal muscle regeneration[J]. Muscle Nerve, 1997, 20(7): 815-822.
[13] Spangenburg EE, Booth FW. Leukemia inhibitory factor restores the hypertrophic response to increased loading in theLIF(-/-)mouse[J]. Cytokine, 2006, 34(3-4): 125-130.
[14] 李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:316.
[15] 贾红玲,张学伟,张永臣.数据挖掘技术在针灸治疗腰痛古代文献中的应用[J].针灸临床杂志,2013,29(11):40-41.
[16] 陈昭品,陈志坚,曾秋棠,等.白细胞介素-17对大鼠心肌梗死后室性心律失常的影响[J].临床心血管病杂志,2012,28(6):422-426.
[17] Nicola NA, Babon JJ. Leukemia inhibitory factor(LIF)[J]. Cytokine Growth Factor Rev, 2015, 26(5): 533-544.
[18] Henningsen J, Rigbolt KT, Blagoev B, et al. Dynamics of the skeletal muscle secretome during myoblastdifferentiation[J]. Mol Cell Proteomics, 2010, 9(11): 2482-2496.
[19] Metcalf D. The unsolved enigmas of leukemia inhibitory factor[J]. Stem cells, 2003, 21(1): 5-14.
[20] Brandt N, O'Neill HM, Kleinert M, et al. Leukemia inhibitory factor increases glucose uptake in mouse skeletal muscle[J]. Am J Physiol Endocrinol Metab, 2015, 309(2):E412-E453.
[21] Brandt N, O'Neill H, Kleinert M, et al. Leukemia inhibitory factor stimulates muscle glucose uptake by a PI3-kinase dependent pathway that is maintained in white muscle in obesity[J]. The Faseb J, 2014, 28: 1162-1164.
[22] Brown MA,Metcalf D, Gough NM. Leukaemia inhibitory factor and interleukin 6 are expressed at very lowlevels in the normal adult mouse and are induced by inflammation[J]. Cytokine, 1994, 6(9): 300-309.
[23] Reardon KA, Kapsa RM, Davis J, et al. Increased levels of leukemia inhibitory factor mRNA in muscularstrophy and human muscletrauma[J]. Muscle Nerve, 2000, 23(6): 962-966.
[24] Kurek JB, Nouri S, Kannourakis G, et al. Leukemia inhibitory factor and interleukin-6 are produced by diseased and regenerating skeletalmuscle[J]. Muscle Nerve,1996, 19(10): 1291-1301.
[25] Srikuea R, Esser KA, Pholpramool C. Leukaemia inhibitory factor is expressed in rat gastrocnemius muscle after contusion and increases proliferation of rat L6 myoblastsvia c-Myc signalling[J]. Clin Exp Pharmacol Physiol, 2011,38(8): 501-509.

相似文献/References:

备注/Memo

备注/Memo:
基金资助: 国家自然科学基金面上项目(81574052)
作者简介: 邹德辉(1989- ),2014级在读硕士研究生。研究方向:针灸临床作用机制研究。E-mail:1198916301@qq.com
通信作者: 张莉(1958- ),女,博士,教授。研究方向:针灸临床作用机制研究。E-mail:zhangli1572@sina.com
更新日期/Last Update: 2017-04-06