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[1]赵正芳 唐纯志.电针关元、三阴交对痛经模型大鼠血清IL-2、5-HT及P物质的影响[J].环球中医药,2017,10(05):541-543.[doi:10.3969/j.issn.1674-1749.2017.05.007]
　ZHAO Zhengfang,TANG Chunzhi..Effect of electroacupuncture at Guanyuan(CV4)and Sanyinjiao(SP6)on serum IL-2, 5-HT and substance P in dysmenorrhea model rats[J].,2017,10(05):541-543.[doi:10.3969/j.issn.1674-1749.2017.05.007]
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电针关元、三阴交对痛经模型大鼠血清IL-2、5-HT及P物质的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年05期
页码:
541-543
栏目:
论著
出版日期:
2017-05-06

文章信息/Info

Title:
Effect of electroacupuncture at Guanyuan(CV4)and Sanyinjiao(SP6)on serum IL-2, 5-HT and substance P in dysmenorrhea model rats
作者:
赵正芳 唐纯志
510006 广州中医药大学针灸康复临床医学院[赵正芳(硕士研究生)、唐纯志]
Author(s):
ZHAO Zhengfang;  TANG Chunzhi.
College of Acupuncture and Moxibustion,Guangzhou University of Chinese Medicine,Guangzhou 510000,China
关键词:
电针;  痛经;  白细胞介素-2;  5-羟色胺;  P物质
Keywords:
Electroacupuncture;  Dysmenorrhea;  Interleukin-2;  5-hydroxytryptamine;  Substance P
分类号:
R245
DOI:
10.3969/j.issn.1674-1749.2017.05.007
文献标志码:
A
摘要:
目的 观察电针关元、三阴交对痛经模型大鼠血清白细胞介素-2(interleukin-2,IL-2)、5-羟色胺(5-hydroxytryptamine,5-HT)及P物质水平的影响,探讨其镇痛作用机制。方法 选用40只健康成年SPF级雌性Sprague-Dawleg大鼠,根据体重随机分为正常对照组、模型组、电针组、布洛芬组,每组10只。模型组、电针组及布洛芬组釆用已烯雌酚和催产素联合制备痛经大鼠模型,电针组给予电针关元、三阴交,布洛芬组给予布洛芬灌胃给药,余组不予治疗。于第11日观察并记录各组30分钟内大鼠扭体反应发生的次数,检测各组大鼠血清内IL-2、5-HT、P物质的含量。结果 经治疗后,痛经模型大鼠的扭体反应发生率明显下降(P＜0.01),电针组大鼠血清中IL-2、5-HT及P物质水平较模型组均有明显改善(P＜0.05)。结论 电针关元、三阴交可明显缓解痛经所致疼痛,其作用机制可能与降低外周炎症致痛因子水平有关。
Abstract:
Objective To observe the effect of electroacupuncture at Guanyuan(CV4)and Sanyinjiao(SP6)in dysmenorrhea rats, to observe the content of IL-2, 5-HT and substance P(SP)in serum and to explore the mechanism of analgesic action. Methods Forty healthy SD female rats(2～3 months, weight of 200～250 g)were randomly divided into normal group, model group, electroacupuncture group and Ibuprofen group, 10 rats in each group. The dysmenorrhea model rats were prepared with Diethylstilbestrol and Oxytocin. The electroacupuncture group rats were treated with electroacupuncture at Guanyuan(CV4)and Sanyinjiao(SP6), and the Ibuprofen group rats were given by Ibuprofen, and the other groups were untreated. The writhing response in 30mins were recorded, and the content of IL-2, 5-HT and substance P(SP)in serum was detected at the eleventh day. Results After treatment, the occurrence rate of writhing response was obviously decreased. Compared with the model group, the content of IL-2, 5-HT and substance P in serum of the electroacupuncture group was significantly decreased(P＜0.05). Conclusion The electroacupuncture can obviously alleviate dysmenorrhea, the mechanism may be related to the reduction of the level of inflammatory factors in peripheral.

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备注/Memo

备注/Memo:
作者简介: 赵正芳(1990- ),女,2014级在读硕士研究生。研究方向:针灸治疗疾病机理。E-mail:491558050@qq.com
通信作者: 唐纯志(1966- ),博士,教授,博士生导师。研究方向:针灸治疗脑病机理。E-mail:jordan664@163.com
更新日期/Last Update: 2017-05-06