|本期目录/Table of Contents|

[1]王思睿 修琳琳 刘殿娜 陈丰 柳海艳 陈绍红 李娜 张晨 高洁 钟赣生.含不同品种海藻与甘草反药组合的海藻玉壶汤对甲状腺肿大大鼠模型的影响[J].环球中医药,2017,10(06):541-545.[doi:10.3969/j.issn.1674-1749.2017.06.001]
　WANG Sirui,XIU Linlin,LIU Dianna,et al.Effect of incompatible herb of different varieties of seaweed combination with glycyrrhiza of Haizao Yuhu decoction on thyromegaly rats model[J].,2017,10(06):541-545.[doi:10.3969/j.issn.1674-1749.2017.06.001]
点击复制

含不同品种海藻与甘草反药组合的海藻玉壶汤对甲状腺肿大大鼠模型的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年06期
页码:
541-545
栏目:
论著
出版日期:
2017-06-06

文章信息/Info

Title:
Effect of incompatible herb of different varieties of seaweed combination with glycyrrhiza of Haizao Yuhu decoction on thyromegaly rats model
作者:
王思睿 修琳琳 刘殿娜 陈丰 柳海艳 陈绍红 李娜 张晨 高洁 钟赣生
100029 北京中医药大学中医学院[王思睿(硕士研究生)、修琳琳(博士研究生)、刘殿娜(博士研究生)、陈丰(硕士研究生)、李娜(硕士研究生)、张晨(硕士研究生)、高洁(博士研究生)、柳海艳、陈绍红、钟赣生]
Author(s):
WANG Sirui;  XIU Linlin;  LIU Dianna; et al.
School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 10029,China
关键词:
海藻;  甘草;  反药组合;  海藻玉壶汤;  甲状腺肿大
Keywords:
Seaweed;  Licorice;  Combination of incompatible herb;  Haizao Yuhu decoction;  Thyromegaly
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.06.001
文献标志码:
A
摘要:
目的 比较不同剂量(接近临床常用剂量)的海藻和甘草对甲状腺肿大大鼠的治疗作用,并探讨海藻的不同品种对治疗作用机制的影响。 方法 大鼠连续灌服丙硫氧嘧啶14天复制甲状腺肿大模型,并将其分为空白组、模型组、阳性对照组、海藻(海蒿子)玉壶汤高剂量组、海藻(海蒿子)玉壶汤中剂量组、海藻(海蒿子)玉壶汤低剂量组、海藻(羊栖菜)玉壶汤高剂量组、海藻(羊栖菜)玉壶汤中剂量组、海藻(羊栖菜)玉壶汤低剂量组,共9组。除空白组外,其余各组大鼠每日灌服丙硫氧嘧啶1次,质量浓度为1 mg/(mL·100 g),连续灌服14天。14天后,空白组和模型组每天灌服等量的生理盐水,其余各组灌服相应药物,体积均为1 mL/100 g,连续给药28天,期间为了稳定模型,每隔1天灌服丙硫氧嘧啶1次,剂量与之前一致。给药结束后,计算各组大鼠甲状腺系数,并测定甲状腺激素三碘甲状腺原氨酸(three iodine thyroid 3,5,3'-triiodothyronine,T3)、四碘甲状腺原氨酸(thyroxine,T4)、游离三碘甲状腺原氨酸(free triiodothyronine,FT3)、游离四碘甲状腺原氨酸(free thyroxine,FT4)、促甲状腺激素(thyroid-stimulating hormone,TSH)、促甲状腺激素释放激素(thyrotropin-releasing hormone,TRH)水平。 结果(1)各给药组T3水平较模型组不同程度升高,其中羊高组略优于海高组,且均分别优于各中、低剂量组(P＜0.05或P＜0.01);(2)各给药组在FT3水平上均体现出向正常转归的趋势,其中海高组优于阳性药组及其他各组(P＜0.05或P＜0.01);(3)与模型组相比,海低组及羊栖菜各组的TSH水平均有显著的下降趋势(P＜0.01),且效果均优于阳性药组,其中羊高组效果最佳。 结论 含不同品种海藻的海藻玉壶汤对由甲状腺肿大引起的各种甲状腺激素水平的变化均有一定程度的恢复作用,其中以海藻玉壶汤(海蒿子)高剂量组、海藻玉壶汤(羊栖菜)高剂量组效果为佳。
Abstract:
Objective To compare the therapeutic effects of different doses(closed to the clinical dose)of seaweed and glycyrrhiza in the treatment of thyromegaly in rats. Methods The thyromegaly model were induced by successive perfusion of propylthiouracil for 14 days, the rats were divided into blank group, model group, positive control group, Haizao Yuhu decoction(Sargassum pallidum)high, medium and low dose group, Haizao Yuhu decoction(Sargassum fusiforme)high, medium and low dose group, total of 9 groups. Except the blank group, the rest rats were intragastrically administered propylthiouracil 1 times a day, the concentration was 1 mg/(mL·100 g), successive perfusion for 14 days. After 14 days, the blank group and the model group were intragastrically administered saline, other groups were treated with corresponding drugs, the volume was 1 mL/100 g, administered continuously for 28 days, in order to stabilize the model, every two days was administered continuously propylthiouracil 1 times during the period. The level of T3,T4,FT3,FT4,TSH and TRH was calculated. Results(1)The levels of T3 in treatment groups were higher than that in model group, while that in Haizao Yuhu decoction(Sargassum fusiforme)high dose group was slightly better than that in Haizao Yuhu decoction(Sargassum pallidum)high dose group, and they were better than those in middle and low dose groups, respectively.(2)The Haizao Yuhu decoction(Sargassum fusiforme)high dose group had the best effect. The FT3 levels in all groups showed a trend toward normal outcome, the Haizao Yuhu decoction(Sargassum pallidum)high dose group was better than other groups.(3)Compared with the model group, the TSH levels in the Haizao Yuhu decoction(Sargassum pallidum)low dose group and Haizao Yuhu decoction(Sargassum fusiforme)groups were significantly decreased, and the effect was better than that of the positive medicine group. Conclusion Different varieties of Haizao Yuhu decoction have a certain degree of restorative effect on the changes of thyroid hormones caused by thyroid enlargement. And the effect of high dose of Haizao Yuhu decoction(Sargassum pallidum)and Haizao Yuhu decoction(Sargassum fusiforme)is the best.

参考文献/References:

[1] 明·陈实功.外科正宗[M].北京:中国中医药出版社,2011:112.
[2] 曾灏,王少丽,林伟根,等.加减海藻玉壶汤治疗肉瘿108例临床疗效分析[J].广东医学,2010,31(8):1050-1052.
[3] 刘洪,陈宏鹏,王宽宇,等.海藻玉壶汤治疗甲状腺腺瘤30例[J].光明中医,2010,26(5):949-950.
[4] 杨建勋.海藻玉壶汤治疗小儿瘰疬50例疗效观察[J].新疆中医,2004,24(1):11.
[5] 李怡文.含海藻与甘草反药组合的海藻玉壶汤应用于甲状腺肿大大鼠模型配伍宜忌条件研究[D].北京:北京中医药大学, 2013.
[6] 国家药典委员会. 中华人民共和国药典(一部)[M]. 北京:中国医药科技出版社, 2015:295.
[7] 曹琰,段金廒,郭建明,等. 不同产地海蒿子、羊栖菜中无机元素的含量分析与评价[J]. 科技导报,2014,32(15):15-24.
[8] 崔征,李玉山,肇文荣,等. 中药海藻及数种同属植物的药理作用[J].中国海洋药物,1997,(3):5-8.
[9] 纪美琳, 许瑞, 王梦,等. 海藻、甘草单用及配伍不同比例对小鼠急性毒性的影响[J]. 南京中医药大学学报自然科学版, 2012, 28(5):452-456.
[10] 赵文娟. 内分泌和代谢病功能检查[M]. 北京: 人民卫生出版社, 2013.
[11] 张霞, 张西增, 王琳琳, 等. 不同甲状腺疾病血清游离三碘甲状腺原氨酸、游离甲状腺素、促甲状腺激素、甲状腺球蛋白检测结果分析[J]. 华西医学, 2010,25(5): 922-923.
[12] 丰琳. 甲状腺疾病患者T3、T4、FT3、FT4、TSH检测结果分析[J]. 中国医药指南, 2013,(19): 609-610.
[13] 格林斯潘. 基础与临床内分泌[M]. 郭晓蕙, 高燕明, 贾伟平, 等译. 北京: 人民卫生出版, 2009.
[14] 郝重耀, 马毅, 李新华, 等. 火针温补法对甲状腺功能减退症大鼠的影响[J]. 中华中医药学刊, 2010,(6): 10-11.
[15] 巫向前.临床检验结果的评价[M].北京:人民卫生出版社,2000.
[16] 高巍, 杨堂河, 孙凤芹. 甲状腺片与优甲乐伍用治疗甲状腺功能减退的疗效观察[J]. 医学理论与实践, 2013,(23): 3138-3139.
[17] 陈煜辉, 方邦江, 周爽. 温肾方药对甲状腺功能减退症大鼠甲状腺素代谢和性激素水平的调节[J]. 中国临床康复, 2006,10(19): 152-154.

相似文献/References:

[1]郭岩松,柳海艳,钟赣生,等.甘遂半夏汤加减甘遂及不同品种甘草对腹水模型大鼠心肝肾功能的影响[J].环球中医药,2016,9(05):527.[doi:10.3969/j.issn.1674-1749.2016.05.003]
　GUO Yan-song,LIU Hai-yan,ZHONG Gan-sheng,et al.Comparing the effect of Gansui Banxia decoction plus or reduce kansui and varieties of glycyrrhiza on heart, liver and kidney function of ascites model rat[J].,2016,9(06):527.[doi:10.3969/j.issn.1674-1749.2016.05.003]
[2]张建美,许皖,钟赣生,等.甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠肝脏病理形态的影响[J].环球中医药,2016,9(05):534.[doi:10.3969/j.issn.1674-1749.2016.05.004]
　ZHANG Jian-mei,XU Wan,ZHONG Gan-sheng,et al.Comparing the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza on pathological morphology of liver of ascites rat under different times[J].,2016,9(06):534.[doi:10.3969/j.issn.1674-1749.2016.05.004]
[3]张建美,许皖,钟赣生,等.甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠水通道蛋白-2基因及肾凋亡的影响[J].环球中医药,2016,9(05):539.[doi:10.3969/j.issn.1674-1749.2016.05.005]
　ZHANG Jian-mei,XU Wan,ZHONG Gan-sheng,et al.Comparing the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza on AQP-2 mRNA expression and apoptosis rate of kidney tissue in ascites rats under different times[J].,2016,9(06):539.[doi:10.3969/j.issn.1674-1749.2016.05.005]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81573630)
作者简介:王思睿(1989- ),女,2014级在读硕士研究生。研究方向:中药药性理论研究。E-mail:153905679@qq.com
通信作者:钟赣生(1961- ),硕士,教授,博士生导师。研究方向:中药药性理论研究。E-mail:zhonggansheng@sohu.com
更新日期/Last Update: 2017-06-06