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[1]杨颖溪 柴立民 晏军 高伟华 吴甜甜 朱紫亨 余佳骏 徐昉.补肾益肺消癥方对琢鄄SMA 及SP鄄C 蛋白对肺纤维化大鼠病变肺组织分布及表达的影响[J].环球中医药,2017,10(06):559-563.[doi:10.3969/j.issn.1674-1749.2017.06.005]
　YANG Yingxi,CHAI Limin,YAN Jun,et al.Influences of Bushen Yifei Xiaozheng decoction on the distribution and expression of α-SMA and SP-C protein in IPF rat[J].,2017,10(06):559-563.[doi:10.3969/j.issn.1674-1749.2017.06.005]
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补肾益肺消癥方对琢鄄SMA 及SP鄄C 蛋白对肺纤维化大鼠病变肺组织分布及表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年06期
页码:
559-563
栏目:
论著
出版日期:
2017-06-06

文章信息/Info

Title:
Influences of Bushen Yifei Xiaozheng decoction on the distribution and expression of α-SMA and SP-C protein in IPF rat
作者:
杨颖溪 柴立民 晏军 高伟华 吴甜甜 朱紫亨 余佳骏 徐昉
450006 河南省郑州市中医院分院老年病科[杨颖溪(硕士研究生)]; 中医内科学教育部和北京市重点实验室(柴立民); 北京中医药大学东直门医院呼吸科[晏军、高伟华、吴甜甜、朱紫亨、余佳骏、徐昉(硕士研究生)]
Author(s):
YANG Yingxi; CHAI Limin; YAN Jun; et al.
Geriatrics Department,Zhengzhou Hospital of traditional Chinese Medicine,Henan 100700,China
关键词:
补肾益肺消癥方;  特发性肺纤维化;  内质网应激;  α-平滑肌肌动蛋白;  肺泡表面活性蛋白C
Keywords:
Bushen Yifei Xiaozheng decoction;  Idiopathic pulmonary fibrosis;  Endoplasmic reticulum stress;  α-SMA;  SP-C
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.06.005
文献标志码:
A
摘要:
目的 探讨补肾益肺消癥方干预肺纤维化大鼠α-SMA及SP-C蛋白在病变肺组织中分布及表达的作用机制。方法 以博来霉素致大鼠肺纤维化的动物模型,以吡非尼酮作为阳性对照药物,补肾益肺消癥方分别于模型过程中、造模后及实验全程进行干预,观察动物活动及体重变化,免疫组化检测病变肺组织中α-SMA、SP-C蛋白表达。结果 大鼠体重显示,模型组体重较正常组明显减轻(P＜0.05),中药防治组体重较模型组明显升高(P＜0.05)。免疫组化、免疫荧光结果显示,模型组病变肺组织中α-SMA、SP-C蛋白表达较正常组明显升高(P＜0.05),阳性组和中药组α-SMA、SP-C蛋白表达较模型组明显减低(P＜0.05)。结论 补肾益肺消癥方可以通过减少α-SMA的表达及成熟SP-C蛋白沉积,减少成纤维灶的形成,抑制内质网应激反应,延缓特发性肺纤维化的病理进程,从而达到治疗的目的。
Abstract:
Objective To investigate the mechanism of Bushen Yifei Xiaozheng decoction in the distribution and expression of α-SMA、SP-C protein of pulmonary fibrosis rats.Methods IPF rat model were induced by Bleomycin. Rats were treated with pirfenidone and Bushen Yifei Xiaozheng decoction respectively before modeling. To observe the changes of activity and the weight of rats during the modeling, after modeling and the whole experiment. The expression of α-SMA and SP-C protein of lung tissue was detected by immunohistochemical staining method. Results The weight of model group was lighter than the normal group(P＜0.05), and the Chinese medicine group gained more weight than the model group(P＜0.05). Compared with the model group,the expression of α-SMA and SP-C were decreased significantly in the treatment group(P＜0.05). Conclusion Bushen Yifei Xiaozheng decoction can reduce the expression of α-SMA and the deposition of the mature SP-C,release the endoplasmic reticulum stress,then to achieve the purpose of treatment.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81373589)
作者简介: 杨颖溪(1990- ),女,2013级在读硕士研究生。研究方向:中医药防治肺系疾病。E-mail:yangyingxi04@163.com
通信作者: 晏军(1968- ),博士,主任医师。研究方向:中医药防治肺系疾病。E-mail:dzmyyyj@126.com
更新日期/Last Update: 2017-06-06