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[1]樊娇娇 李发荣 赵崇军 徐柯心 杨冉 乔艺涵 倪媛媛 王雨彤 马志强 林瑞超.盐酸肾上腺素诱导建立斑马鱼血栓模型的优化与适用性研究[J].环球中医药,2017,10(06):563-568.[doi:10.3969/j.issn.1674-1749.2017.06.006]
　FAN Jiaojiao,LI Farong,ZHAO Chongjun,et al.Optimization and adaptability of the zebrafish thrombosis model induced by Adrenaline Hydrochloride[J].,2017,10(06):563-568.[doi:10.3969/j.issn.1674-1749.2017.06.006]
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盐酸肾上腺素诱导建立斑马鱼血栓模型的优化与适用性研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年06期
页码:
563-568
栏目:
论著
出版日期:
2017-06-06

文章信息/Info

Title:
Optimization and adaptability of the zebrafish thrombosis model induced by Adrenaline Hydrochloride
作者:
樊娇娇 李发荣 赵崇军 徐柯心 杨冉 乔艺涵 倪媛媛 王雨彤 马志强 林瑞超
100029 北京中医药大学中药学院中药品质评价局北京市重点实验室[樊娇娇(硕士研究生)、赵崇军(博士研究生)、徐柯心(硕士研究生)、杨冉(硕士研究生)、乔艺涵(硕士研究生)、倪媛媛(硕士研究生)、王雨彤(硕士研究生)、马志强、林瑞超]; 陕西师范大学(李发荣)
Author(s):
FAN Jiaojiao; LI Farong; ZHAO Chongjun; et al.
Beijing Key Laboratory of Traditional Chinese Quality Evaluation,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China
关键词:
盐酸肾上腺素;  斑马鱼血栓模型;  定量;  抗血栓活性;  药物筛选
Keywords:
Adrenaline Hydrochloride;  Zebrafish thrombosis model;  Quantitative;  Antithrombotic activity;  Drug screening
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.06.006
文献标志码:
A
摘要:
目的 优化盐酸肾上腺素诱导建立斑马鱼血栓模型的条件,并考察此模型在中药抗血栓活性筛选中的适用性。方法 应用正交实验,以斑马鱼尾静脉红细胞的染色强度为定量指标,考察盐酸肾上腺素诱导建立斑马鱼血栓模型的最佳条件。利用该模型,对临床常用的11味中药的抗血栓活性进行筛选,探讨该模型在中药抗血栓活性筛选中的适用性与可靠性。结果 盐酸肾上腺素诱导建立斑马鱼血栓模型的最佳条件为:受精后3天(3 dayspost fertilization,3dpf)的斑马鱼在浓度为15 μmol/L盐酸肾上腺素溶液中暴露16小时。9味活血化瘀中药川芎、郁金、延胡索、丹参、红花、虎杖、三棱、莪术、水蛭在斑马鱼血栓模型中表现出显著的预防和治疗血栓作用,而甘草和党参两味中药在斑马鱼血栓模型中未表现出预防和治疗血栓的作用。结论 盐酸肾上腺素诱导建立的斑马鱼血栓模型在中药抗血栓活性的筛选与疗效评估中具有良好的适用性。
Abstract:
Objective To optimize the conditions of the zebrafish thrombosis model induced by Adrenaline Hydrochloride, and investigate the adaptability of the model in antithrombotic activity screening of traditional Chinese medicine. Methods This study applied orthogonal experiment to inspect the optimum conditions of the zebrafish thrombosis model induced by Adrenaline Hydrochloride. Quantitative index was quantitatively evaluated by the dyeing strength of zebrafish tail vein red blood cells. The model was used to screen the antithrombotic activity of 11 herbs, and applicability and reliability was discussed. Results Zebrafish at 3dpf(days post-fertilization,3dpf)treated with Adrenaline Hydrochloride at a concentration of 15 μmol·L-1 for a time period of 16 hours were determined as the optimum conditions for the zebrafish thrombosis model development. 9 kinds of traditional Chinese medicine with invigorating blood circulation and eliminating blood stasis, such as, Salvia miltiorrhiza, Sparganii Rhizoma, Ligusticum chuanxiong Hort, etc showed significant preventive and therapeutic effects on zebrafish thrombosis model. However, Glycyrrhizae Radix and Codonopsis pilosula did not display the effect on this zebrafish thrombosis model. Conclusion Zebrafish thrombosis model induced by Adrenaline Hydrochloride has good applicability in antithrombotic activity screening and efficacy assessments of traditional Chinese medicine.

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备注/Memo

备注/Memo:
基金项目: 北京市科委创新环境与平台建设专项(Z16111000500000)
作者简介: 樊娇娇(1989- ),女,2014级在读硕士研究生。研究方向:中药毒性与活性。E-mail:276050202@qq.com
通信作者: 林瑞超(1954- ),博士,教授,博士生导师。研究方向:中药、天然药物的质量控制方法的研究。E-mail:linrch307@sina.com
更新日期/Last Update: 2017-06-06