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[1]武迎磊 赵天 李明.灯盏花素对大鼠动脉粥样硬化保护作用的研究[J].环球中医药,2017,10(06):569-573.[doi:10.3969/j.issn.1674-1749.2017.06.007]
　WU Yinglei,ZHAO Tian,LI Ming..Protective effects and mechanism of breviscapine on atherosclerosis rats[J].,2017,10(06):569-573.[doi:10.3969/j.issn.1674-1749.2017.06.007]
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灯盏花素对大鼠动脉粥样硬化保护作用的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年06期
页码:
569-573
栏目:
论著
出版日期:
2017-06-06

文章信息/Info

Title:
Protective effects and mechanism of breviscapine on atherosclerosis rats
作者:
武迎磊 赵天 李明
054000 邢台市第三医院药剂科(赵天、武迎磊); 沧州市食品药品检验所(李明)
Author(s):
WU Yinglei;  ZHAO Tian; LI Ming.
The third hospital of Xingtai,Xingtai 054000, China
关键词:
灯盏花素;  动脉粥样硬化;  血脂;  炎症;  氧化应激
Keywords:
Breviscapine;  Atherosclerosis;  Blood lipid;  Inflammation;  Oxidative stress
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.06.007
文献标志码:
A
摘要:
目的 研究灯盏花素(breviscapine,Bre)对大鼠动脉粥样硬化的保护作用。方法 采用高脂饲料喂养结合腹腔注射维生素D3(VD3)的方法建立动脉粥样硬化大鼠模型,分别于造模过程同步灌胃给予Bre 6、12、24 mg·(kg·d)-1和辛伐他汀SV 1.8 mg·(kg·d)-1,共12周,并设模型组和正常对照组。计算各组大鼠心脏指数(cardiac index,CI),通过HE染色观察主动脉形态变化并进行病变分级,生化分析法测定血清血脂指标,比色法测定血浆炎症细胞因子含量水平和血清中抗氧化酶活性及丙二醛(MDA)含量。结果 与模型组比较发现Bre 12、24 mg·(kg·d)-1组动脉粥样硬化大鼠CI显著降低(P＜0.05); Bre各组大鼠主动脉病变呈不同程度改善,Bre 24 mg·(kg·d)-1组大鼠主动脉病变分级水平显著降低(P＜0.05); Bre 12、24 mg·(kg·d)-1组血清血脂指标TC、TG、LDL-C含量显著降低(P＜0.05),血浆炎症细胞因子CRP、TNF-α、IL-1β、IL-6含量明显降低(P＜0.05),血清抗氧化酶SOD、CAT活性显著升高且MDA含量显著降低(P＜0.05); Bre 24 mg·(kg·d)-1组血清血脂指标HDL-C含量和抗氧化酶GSH-Px活性均显著升高(P＜0.05),血浆中炎症细胞因子IL-10含量显著升高(P＜0.05)。结论 Bre对大鼠动脉粥样硬化具有一定的保护作用,其机制可能与Bre能够有效调节血脂、抑制炎症反应和降低氧化应激损伤有关。
Abstract:
Objective To investigate the protective effects and mechanism of breviscapine(Bre)on atherosclerosis rats. Methods The atherosclerosis models were made by high-fat diet and intraperitoneal injection with VD3, and at the same time, the rats were treated with Bre[6, 12, 24 mg·(kg·d)-1] and simvastatin[SV, 1.8 mg·(kg·d)-1], course of the treatment was 12 weeks; the rats were divided into normal control group and model group. The cardiac index(CI)was calculated; the histopathological changes and lesion classification of abdominal aorta was observed by HE staining; the serum lipids in serum were detected; the level of inflammatory cytokines in serum were determined; the activity of antioxidase and the content of MDA in serum were determined. Results Compared with model group, the CI of Bre[12, 24 mg·(kg·d)-1] groups were significantly decreased(P＜0.05); the histopathological changes were significantly improved; the level of TC, TG, LDL-C in serum of Bre[12, 24 mg·(kg·d)-1] groups were significantly decreased(P＜0.05); the level of CRP, TNF-α, IL-1β, IL-6 in plasma were significantly decreased(P＜0.05); the activity of SOD, CAT in serum were significantly increased while the content of MDA was significantly decreased(P＜0.05,P＜0.05); the level of HDL-C and the activity of GSH-Px in Bre 24 mg·(kg·d)-1 group was significantly increased(P＜0.05), the level of IL-10 was significantly increased(P＜0.05). Conclusion Bre has protective effects on atherosclerosis rats, which perhaps related to its effects of reducing cholesterol, improving the anti-inflammatory and antioxidant capacity.

参考文献/References:

[1] 聂永梅,成敏,陈槐卿. 动脉粥样硬化进程中血脂、血流动力学指标和血管内膜的动态变化[J]. 生物医学工程学杂志,2005,22(1):10-14.
[2] Landmesser U,Spiekermann S,Dikalov S,et al. Vascular oxidative stress and endothelial dysfunction in patients with chronic heart failure:role of xanthine-oxidase and extracellular superoxide dismutase[J]. Circulation,2002,106(24):3073-3078.
[3] Stocker R,Keaney JF.Role of oxidative modifications in atherosclerosis[J]. Physiological Reviews,2004,84(4):1381-1478.
[4] 丁宁. 灯盏花素注射液对痛风患者抗氧化能力的影响研究[J]. 中国现代药物应用,2016,10(10):150-151.
[5] 李军,吴立友,王朝阳. 灯盏花素注射液对 2 型糖尿病早期肾病患者相关炎症因子的影响[J]. 中药药理与临床,2011,27(3):110-112.
[6] 金桂兰,覃慧林,石孟琼,等. 灯盏花素对小鼠局灶性脑缺血损伤的保护作用及与脑组织P-糖蛋白表达研究[J]. 中药药理与临床,2015,31(1):76-79.
[7] 杨宏辉,高传玉,徐予,等. 动脉粥样硬化SD大鼠模型的建立[J]. 医药论坛杂志,2009,30(2):18-22.
[8] 张凤华,楚溪,张俊芳,等. 调脂通脉汤对动脉粥样硬化大鼠血流动力学的影响[J]. 时珍国医国药,2012,23(2):355-356.
[9] Schfers M,Schober O,Hermann S. Matrix-metalloproteinases as imaging targets for inflammatory activity in atherosclerotic plaques[J]. J Nucl Med,2010,51(5):663-666.
[10] 郑楚,杨冬业,徐勤,等. 三七花总皂苷对动脉粥样硬化模型大鼠血脂及血液流变学影响[J]. 中国实验方剂学杂志,2010,16(12):162-164.
[11] 王苑铭,张朝宁,余臣祖,等. 大黄多糖对糖尿病动脉粥样硬化大鼠血脂代谢及主动脉形态学的影响[J]. 中国中医药信息杂志,2012,19(9):48-49.
[12] 张晶,关欣,王伟,等. 血脂代谢与颈动脉粥样硬化不稳定性斑块的相关性[J]. 中国动脉硬化杂志,2012,20(9):833-836.
[13] 王燕. 血液流变学指标与高脂血症的关系[J]. 检验医学与临床,2007,4(12):1179-1180.
[14] 袁庆芳,石国锋. 瑞舒伐他汀钙对脑梗死患者动脉粥样硬化和脑血流动力学的影响[J]. 现代中西医结合杂志,2013,22(20):2178-2180.
[15] 蔡劲,施园. 针灸对颈动脉粥样硬化形态和血流动力学影响的临床研究[J]. 中华中医药学刊,2013,31(8):1695-1697.
[16] Fatar M,Stroick M,Griebe M,et al. Matrix metallproteinases in cerebrovascular diseases[J]. Cerebrovaso Dis,2005,20(3):141-151.
[17] Considine MJ,Sandalio LM,Foyer CH. Unravelling how plants benefit from ROS and NO reactions,while resisting oxidative stress[J]. Ann Bot,2015,116(4):469-473.
[18] Lartigue A,Burlat B,Coutard B,et al. The megavirus chilensis Cu,Zn-superoxide dismutase:the first viral structure of a typical CCS-independent hyperstable dimeric enzyme[J]. J Virol, 2014,2588(14):254-261.
[19] Li X,Zhao L,Yue L,at al. Evidence for the protective effects of curcumin against oxyhemoglobin-induced injury in rat cortical neurons[J]. Brain Res Bull,2016,120:34-40.

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备注/Memo

备注/Memo:
作者简介: 武迎磊(1984- ),本科,主管药师。研究方向:临床药学。E-mail:yimy21@126.com
通信作者: 李明(1983- ),本科,主管药师。研究方向:药物分析药理学研究。E-mail:czhlm2016@163.com
更新日期/Last Update: 2017-06-06