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[1]马梦雨 杨晓燕 赵璐 梁芳 张勇 徐可 樊瀛哲 许建华.肝气郁结对结肠癌模型小鼠肝转移的影响[J].环球中医药,2017,10(06):582-586.[doi:10.3969/j.issn.1674-1749.2017.06.010]
　MA Mengyu,YANG Xiaoyan,ZHAO Lu,et al.Effect of liver qi stagnationon liver metastasis of mouse model of colon cancer[J].,2017,10(06):582-586.[doi:10.3969/j.issn.1674-1749.2017.06.010]
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肝气郁结对结肠癌模型小鼠肝转移的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年06期
页码:
582-586
栏目:
论著
出版日期:
2017-06-06

文章信息/Info

Title:
Effect of liver qi stagnationon liver metastasis of mouse model of colon cancer
作者:
马梦雨 杨晓燕 赵璐 梁芳 张勇 徐可 樊瀛哲 许建华
200062 上海中医药大学附属普陀医院中医肿瘤科[马梦雨(博士研究生)、梁芳、张勇、徐可、樊瀛哲、许建华],人事处(杨晓燕); 无锡市中医医院肿瘤科(赵璐)
Author(s):
MA Mengyu;  YANG Xiaoyan;  ZHAO Lu;  et al.
Department of Oncology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai,201203,China
关键词:
肝气郁结;  结肠癌;  肝转移;  β-AR信号通路
Keywords:
Liver qi stagnation;  Colon cancer;  Liver metastasis;  β-AR signal pathway
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.06.010
文献标志码:
A
摘要:
目的 通过构建肝气郁结证模型,观察肝气郁结对结肠癌肝转移的影响并探讨β-AR信号通路在其中的作用机制。 方法 通过束缚制动法构建肝气郁结证模型,将BALB/C裸小鼠随机分为空白对照组、普萘洛尔对照组、ICI118,551 对照组、空白肝郁组、普萘洛尔肝郁组、ICI118,551 肝郁组,共六组,每组6只。ELISA法检测血清、脾种植瘤肾上腺素(epinephrine,E)、去甲肾上腺素(norepinephrine,NE)含量; qRT-PCR 和Western blot 法检测脾脏种植瘤组织转化生长因子-β(transforming growth factor-β,TGF-β)、白介素-6(interleukin-6,IL-6)、血管内皮生长因子(vascular endothelial growth factor,VEGF)和基质金属蛋白酶-9(matrix metalloprotein-9,MMP-9)表达情况; 免疫组化法观察肝转移瘤组织VEGF和CD31蛋白表达情况。 结果 与空白对照组相比,空白肝郁组小鼠肝脏重量明显升高(P＜0.01),并能升高小鼠血清NE、E水平及脾脏NE水平(P＜0.01、P＜0.05、P＜0.05),差异具有统计学意义; 与对照组相比,肝郁组小鼠脾脏肿瘤组织中TGF-β、IL-6、VEGF、MMP-9蛋白以及mRNA的表达显著升高(P＜0.01),而给予普萘洛尔或ICI118,551后表达降低(P＜0.01、P＜0.05),差异具有统计学意义; 肝郁组肝转移瘤VEGF、CD31表达明显高于对照组(P＜0.01),给予普萘洛尔或ICI118,551后,则表达降低(P＜0.01、P＜0.05),差异有统计学意义。 结论 肝气郁结可通过介导β2-AR信号通路促进结肠癌肝转移。
Abstract:
Objective The liver qi stagnation syndrome model was established to observe the effect of liver qi stagnation on liver metastasis of colon cancer and the role of β-AR signal pathway in it. Methods BALB/C mice were randomly divided into blank control group, propranolol control group, ICI118,551 control group, liver depression group, liver depression treated with propranolol group, liver depression treated with ICI118,551 group. The expressions of TGF-β, IL-6, VEGF and MMP-9 were detected by ELISA and Western blot. The expressions of VEGF and CD31 in liver metastatic tissues were observed by immunohistochemistry. Results Compared with the blank control group, the liver weight of the mice was significantly increased of liver depression group(P＜0.01), and the level of NE and E in blood was increased(P＜0.01, P＜0.05)and the level of NE in spleen was increased(P＜0.05). The expression of TGF-β, IL-6, VEGF, MMP-9 and mRNA in spleen tumor of liver depression group was significantly higher than those of control group(P＜0.01), and the expression was decreased after treated with propranolol or ICI118,551(P＜0.01, P＜0.05). The expression of VEGF and CD31 in liver metastases of liver depression group was significantly higher than those in control group(P＜0.01), and the expression was decreased after treated with propranolol or ICI118,551(P＜0.01, P＜0.05).Conclusion Liver qi stagnation can promote liver metastasis of colorectal carcinoma by mediating β2-AR signal pathway.

参考文献/References:

[1] Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013[J]. CA Cancer J Clini,2013,63(1):11-30.
[2] 汤钊猷. 现代肿瘤学[M].上海:复旦大学出版社, 2011:954-1018.
[3] 陈家旭, 杨维益. 神经-内分泌-免疫网络研究概括及其与中医肝脏关系的探讨[J]. 北京中医药大学学报,1995,18(4):7-11.
[4] Rodriguez C, Jacobs EJ, Deka A, et al. Use of blood-pressure-lowering medication and risk of prostate cancer in the Cancer Prevention Study II Nutrition Cohort[J]. Cancer Causes Control,2009,20(5):671-679.
[5] Powe DG, Voss MJ, Zanker KS, et al. Beta-blocker drug therapy reduces secondary cancer formation in breast cancer and improves cancer specific survival[J]. Oncotarget,2010,1(7):628-638.
[6] Ganz PA, Habel LA, Weltzien EK,et al. Examining the influence of beta blockers and ACE inhibitors on the risk for breast cancer recurrence: results from the LACE cohort[J]. Breast Cancer Res Treat,2011,129(2):549-556.
[7] Barron TI, Connolly RM, Sharp L, et al. Beta blockers and breast cancer mortality: a population-based study[J]. J Clin Oncol,2011,29(19):2635-2644.
[8] Melhem-Bertrandt A, Chavez-Macgregor M, Lei X, et al. Beta-blocker use is associated with improved relapse-free survival in patients with triple-negative breast cancer[J]. J Clin Oncol,2011,29(19):2645-2652.
[9] Lemeshow S, Sørensen HT, Phillips G, et al. β-Blockers and survival among Danish patients with malignant melanoma: a population-based cohort study[J]. Cancer Epidemiol Biomarkers Prev,2011,20(10):2273-2279.
[10] De Giorgi V, Grazzini M, Gandini S, et al. Treatment with β-blockers and reduced disease progression in patients with thick melanoma[J]. Arch Intern Med,2011,171(8):779-781.
[11] Perron L, Bairati I, Harel F, et al. Antihypertensive drug use and the risk of prostate cancer(Canada)[J]. Cancer Causes Control,2004,15(6):535-541.
[12] 徐建阳.中医肝郁证的动物模型研究[J]. 广州中医药大学学报,2004,21(1):73-76.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金面上项目(81573940); 国家自然科学基金青年项目(81403350); 上海市进一步加快中医药事业发展三年行动计划(中医药专门人才计划项目)(ZY3-RCPY-3-1012)
作者简介: 马梦雨(1989- ),女,2014级在读博士研究生。研究方向:中医内科学。E-mail:intercity@163.com
通信作者: 许建华(1962- ),博士,主任医师,博士生导师。研究方向:中医肿瘤学。E-mail:xujianhua50@126.com
更新日期/Last Update: 2017-06-06