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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第10卷

期数:

2017年06期

页码:

592-596

栏目:

论著

出版日期:

2017-06-06

文章信息/Info

Title:

Effect of N-butanolextract from Lianhua decoction on proliferation and apoptosis of human endometrial cancer cell

作者:

唐瑶 包晓霞 鲁周南 薛晓鸥

100700 北京中医药大学东直门医院妇科[唐瑶(博士研究生)、包晓霞、鲁周南、薛晓鸥]

Author(s):

TANG Yao; BAO Xiaoxia; LU Zhounan; et al.

Dongzhimen Hospital Bejing University of Chinese Medicine,Beijing 100700,China

关键词:

子宫内膜癌;  “连花汤”正丁醇提物;  细胞增殖;  细胞凋亡

Keywords:

Endometrial cancer;  N-butanol extract from Lianhua decoction;  Cell proliferation;  Cell apoptosis

分类号:

R711.74

DOI:

10.3969/j.issn.1674-1749.2017.06.012

文献标志码:

A

摘要:

目的 研究“连花汤”正丁醇提物对子宫内膜癌细胞增殖和凋亡的影响,为临床应用“连花汤”治疗子宫内膜癌提供实验依据。方法 提取物从10 mg/mL依次降浓度梯度至0.078125 mg/mL分成8组,即:10 mg/mL、5 mg/mL、2.5 mg/mL、1.25 mg/mL、0.625 mg/mL、0.3125 mg/mL、0.15625 mg/mL、0.078125 mg/mL组。MTT法检测细胞抑制率; 分别计算出两种细胞的半数抑制浓度(IC₅₀),流式细胞术检测细胞凋亡率。结果 “连花汤”正丁醇提物在不同浓度、不同时间里对2种内膜癌细胞系的增殖有显著抑制作用。其中,作用24小时、48小时,HEC-1A细胞1.25 mg、0.625 mg、0.3125 mg组间细胞抑制率有显著性差异(P＜0.01); Ishikawa细胞5 mg/mL、2.5 mg/mL、1.25 mg/mL、0.625 mg/mL、0.3125 mg/mL、0.15625 mg/mL组间细胞抑制率有显著性差异(P＜0.01); 作用24小时,HEC-1A细胞、Ishikawa细胞10 mg、5 mg、0.625 mg、0.078125 mg组间细胞抑制率有显著性差异(P＜0.01)。与正常组相比,“连花汤”正丁醇提物细胞凋亡率显著增高,差异显著。其中,HEC-1A细胞:高剂量组0.4 mg/mL、中剂量组0.2 mg/mL、低剂量组0.1 mg/mL与正常组比较,细胞凋亡率有显著性差异(P＜0.01); Ishikawa细胞:Ishikawa 高剂量组1.0 mg/mL、中剂量组0.5 mg/mL、低剂量组0.25 mg/mL与正常组比较,细胞凋亡率有显著性差异(P＜0.05)。结论 “连花汤”正丁醇提物能有效抑制子宫内膜癌细胞增殖、诱导细胞凋亡。

Abstract:

Objective To investigate the effect of N-butanol extract from Lianhua decoction on proliferation and apoptosis of human endometrial cancer cell, and provide the experimental evidence of endometrial cancer treatment.Methods N-butanol extract from Lianhua decoction was divided into 10 mg/mL,5 mg/mL,2.5 mg/mL,1.25 mg/mL,0.625 mg/mL,0.3125 mg/mL,0.15625 mg/mL,0.078125 mg/mL gourps. Half inhibition concentration(IC₅₀)of cell was calculated, apoptosis rate was detected by flow cytometry.Results N-butanol extract from Lianhua decoction had significant inhibitory effects on the proliferation of two kinds of endometrial cancer cell lines at different concentrations and different time. The inhibition rates of HEC-1A cells in 1.25 mg gourp, 0.625 mg gourp, 0.3125 mg gourp were significantly different at 24 hours and 48 hours(P＜0.01). Cell inhibition rate of Ishikawa cell in 5 mg/mL gourp, 2.5 mg/mL gourp, 1.25 mg/mL gourp, 0.625 mg/mL gourp, 0.3125 mg/ml gourp, 0.15625 mg/mL gourp had significant difference(P＜0.01,P＜0.01). Cell inhibition rate of HEC - 1 A cells and Ishikawa cells in 10 mg gourp, 5 mg gourp, 0.625 mg gourp, 0.078125 mg gourp had significant difference in 24 hours(P＜0.01). Compared with normal group, the apoptosis rate of N-butyl alcohol extract from Lianhua decoctionon was significantly higher. The cell apoptosis rate of high dose of 0.4 mg/mL, middle dose group of 0.2 mg/mL and low dose of 0.1 mg/mL had significant difference compared with normal group in HEC-1A cell(P＜0.01). The cell apoptosis rate of high dose of 1.0 mg/mL, middle dose group of 0.5 mg/mL and low dose group of 0.25 mg/mL had significant difference compared with normal group in Ishikawa cell(P＜0.05).Conclusion The N-butanol extract from Lianhua decoction can effectively inhibit the cell proliferation and induce cell apoptosis.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目: 国家自然科学基金青年科学基金(81403262)
作者简介: 唐瑶(1985- ),2014级在读博士研究生。研究方向:中西医结合治疗妇科肿瘤。E-mail:tangyaobeijing@126.com
通信作者: 薛晓鸥(1963- ),女,博士,教授,主任医师。研究方向:中西医结合治疗妇科内分泌及肿瘤。E-mail:Pro_xue@163.com

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更新日期/Last Update: 2017-06-06