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[1]张颖 周明学 李思耐 任攀 刘卫红 尚菊菊 刘红旭.参元丹胶囊对动脉粥样硬化小鼠主动脉斑块的干预作用及胰岛素抵抗标志蛋白GLUT-4的影响[J].环球中医药,2017,10(07):677-682.[doi:10.3969/j.issn.1674-1749.2017.07.001]
　ZHANG Ying,ZHOU Mingxue,LI Sinai,et al.Effects of Shenyuandan capsule on atherosclerotic plaque and the expression of GLUT-4 in atherosclerotic mice[J].,2017,10(07):677-682.[doi:10.3969/j.issn.1674-1749.2017.07.001]
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参元丹胶囊对动脉粥样硬化小鼠主动脉斑块的干预作用及胰岛素抵抗标志蛋白GLUT-4的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年07期
页码:
677-682
栏目:
论著
出版日期:
2017-07-06

文章信息/Info

Title:
Effects of Shenyuandan capsule on atherosclerotic plaque and the expression of GLUT-4 in atherosclerotic mice
作者:
张颖 周明学 李思耐 任攀 刘卫红 尚菊菊 刘红旭
100149 首都医科大学附属北京潞河医院中医科(张颖); 首都医科大学附属北京中医医院 北京市中医研究所(周明学、李思耐、任攀、刘卫红),首都医科大学附属北京中医医院中医心内科(刘红旭、尚菊菊)
Author(s):
ZHANG Ying; ZHOU Mingxue; LI Sinai; et al.
Department of TCM,Beijing Luhe Hospital, Capital Medical University,Beijing 100149,China
关键词:
动脉粥样硬化;  参元丹胶囊;  血脂异常;  胰岛素抵抗;  葡萄糖转运蛋白-4
Keywords:
Atherosclerosis;  Shenyuandan capsule;  Dyslipidemia;  Insulin resistance;  Glucose transport protein-4
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.07.001
文献标志码:
A
摘要:
目的 参元丹胶囊对动脉粥样硬化(atherosclerosis,AS)小鼠主动脉斑块的干预作用及胰岛素抵抗标志蛋白葡萄糖转运蛋白-4(glucose transport protein-4,GLUT-4)的影响。方法 将50只8周龄雄性ApoE-/-小鼠西方类型膳食喂养6周后随机分为模型组、立普妥(阳性对照组)、参元丹高剂量组、中剂量组和低剂量组(n=10),给予药物腹腔注射6周。检测其血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、血清游离脂肪酸、空腹血糖和空腹血清胰岛素水平。行苏木素-伊红染色(hematoxylin and eosin staining, HE),图像分析测量小鼠主动脉AS斑块面积。免疫组化法检测各组小鼠主动AS斑块内GLUT-4的表达。结果 与模型组相比,参元丹胶囊中剂量组小鼠血清TC水平明显降低(P＜0.01)、参元丹胶囊中、低剂量组小鼠血清TG水平明显降低(P＜0.05)、参元丹胶囊3个剂量组LDL-C水平明显降低(P＜0.05,),参元丹胶囊中、低剂量组HDL-C水平明显升高(P＜0.05),参元丹胶囊3个剂量组小鼠动脉粥样硬化指数显著降低(P＜0.05),高、中、低剂量组小鼠血清游离脂肪酸浓度显著降低(P＜0.05)。参元丹胶囊3个剂量组小鼠空腹血糖水平无显著差异(P＞0.05),血清胰岛素水平显著降低(P＜0.05),中剂量组胰岛素抵抗指数显著降低(P＜0.01)。与模型组相比,参元丹胶囊中、高剂量组小鼠主动脉斑块内GLUT-4表达显著升高(P＜0.05)。结论 参元丹胶囊可能通过调控GLUT-4表达改善胰岛素抵抗,从而达到抗AS的作用。
Abstract:
Objective To study the effect of Shenyuandan capsule on atherosclerotic plaque and the expressions of insulin resistance marker protein glucose transport protein-4(GLUT-4)in aorta of atherosclerotic mice. Methods Fifty 8 week-old male ApoE-/- mice were fed with a western type diet for 6 weeks, and the rats were randomly divided into control group, Lipitor(positive control group), Shenyuandan capsule(high-dose group, SYDC-H, middle-dose group, SYDC-M,and low-dose group, SYDC-L ), 10 rats in each group, and have been intraperitoneally injected for 6 weeks. The levels of total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), serum free fatty acid, fasting blood glucose and fasting insulin levels in serum of ApoE-/- mice were detected. HE staining was used to measure the area of AS plaque in aortic. Immunohistochemical method was used to detect the expression of GLUT-4 in plaques of AS mice. Results Compared with the control group, the TC levels of SYDC-M group were significantly reduced, the TG levels of the mice of SYDC-M group and SYDC-L group were significantly decreased, the LDL-C levels of SYDC-L group,SYDC-M group and SYDC-H group were significantly decreased and the HDL-C levels of SYDC-M group and SYDC-L were significantly increased(P＜0.05). The atherosclerosis indexes of SYDC-L group,SYDC-M group and SYDC-H group were decreased significantly(P＜0.05). The levels of free fatty acids in serum of SYDC-L group,SYDC-M group and SYDC-H group were significantly decreased(P＜0.05). There was no significant difference in fasting plasma glucose level(P＞0.05), the insulin level of SYDC-H group, SYDC-M group and SYDC-L group were significantly decreased(P＜0.05)and the insulin resistance indexes of SYDC-M group were significantly decreased(P＜0.05). Compared with the control group, the expressions of GLUT-4 in the aortic plaques of SYDC-H group and SYDC-M group were significantly increased(P＜0.05). Conclusion Shenyuandan capsule may be possible to improve the insulin resistance by regulating the expression of GLUT-4, so as to achieve the effect of anti AS.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 81673744); 北京市自然科学基金(7162043); 北京市卫生系统高层次卫生技术人才项目( 2014-3-100)
作者简介: 张颖(1979- ),女,博士,主治医师。研究方向:中西医结合心内科研究。E-mail:zhyxr15@126.com
通信作者: 刘红旭(1963- ),本科,教授。研究方向:心血管疾病的中西医结合研究。E-mail:lxh_263.net
更新日期/Last Update: 2017-07-06