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[1]刘浩龙 王家典 卢鋆 翟鹏云 赵晖 李明 王蕾.右归丸“阴中求阳”配伍对肾阳虚大鼠下丘脑-垂体-肾上腺轴的作用[J].环球中医药,2017,10(09):950-953.[doi:10.3969/j.issn.1674-1749.2017.09.004]
　LIU Haolong,WANG Jiadian,LU Yun,et al.Effect of compatibility of reinforcing yang from yin of Yougui pill on hypothalamic-pituitary-adrenal axis in rats with kidney-yang deficiency[J].,2017,10(09):950-953.[doi:10.3969/j.issn.1674-1749.2017.09.004]
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右归丸“阴中求阳”配伍对肾阳虚大鼠下丘脑-垂体-肾上腺轴的作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年09期
页码:
950-953
栏目:
论著
出版日期:
2017-09-06

文章信息/Info

Title:
Effect of compatibility of reinforcing yang from yin of Yougui pill on hypothalamic-pituitary-adrenal axis in rats with kidney-yang deficiency
作者:
刘浩龙 王家典 卢鋆 翟鹏云 赵晖 李明 王蕾
100069 北京,首都医科大学中医药学院 中医络病研究北京市重点实验室[刘浩龙(博士研究生)、王家典(本科生)、卢鋆(本科生)、翟鹏云(本科生)、赵晖、李明、王蕾]
Author(s):
LIU Haolong; WANG Jiadian; LU Yun; et al.
School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, China
关键词:
右归丸及其拆方;  肾阳虚大鼠;  下丘脑-垂体-肾上腺轴
Keywords:
Yougui pill and its disassembled formulas;  Rats with kidney-yang deficiency;  HPA axis
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.09.004
文献标志码:
A
摘要:
目的 探究右归丸及其拆方滋阴和补阳对肾阳虚大鼠下丘脑-垂体-肾上腺轴的调节作用,阐释右归丸“阴中求阳”理论的科学内涵。方法 将30只雄性Wistar大鼠随机分为正常组、模型组、右归丸组、滋阴方组和补阳方组5组,每组6只。采用肌肉注射氢化可的松注射液的方法建立大鼠肾阳虚模型。观察各组大鼠体重、胸腺、肾上腺和脾重量变化; 采用放免法测定各组大鼠下丘脑促肾上腺皮质激素释放激素(corticotropin releasing hormone,CRH),血清促肾上腺皮质激素(adreno cortico hormones,ACTH)和皮质酮(Corticosterone,Cort)含量。结果 右归丸及其拆方对肾阳虚大鼠的体重、胸腺、肾上腺和脾重量有不同程度的改善作用,并且对大鼠下丘脑CRH、血清ACTH和Cort的含量也有调节作用,而右归丸在调节CRH和Cort方面的作用优于滋阴方。结论 右归丸及其拆方均具有改善肾阳虚大鼠的作用,可能与调节肾阳虚大鼠下丘脑-垂体-肾上腺轴有关。右归丸治疗作用优于滋阴方或补阳方。
Abstract:
Objectives To study the effect of Yougui pill(YGP)and its disassembled formulas of nourishing yin(NY)and tonifying yang(TY)on regulation of hypothalamic-pituitary-adrenal(HPA)axis in rats with kidney-yang deficiency and illuminate the scientific meaning of reinforcing yang from yin. Methods The 30 Wistar male rats were randomly divided into 5 groups: normal group, model group(MO), YGP group, NY group and TY group, 6 rats in each group. The kidney-yang deficiency rat model was induced by intramuscular injection with hydrocortisone. The changes of weight, thymus gland, adrenal gland and spleen were observed. The corticotropin releasing hormone(CRH)in hypothalamus, corticosterone(Cort)and adrenocorticotropic hormone(ACTH)in serum were detected by radioimmunoassay. Results The weight of rat, thymus gland, adrenal gland and spleen were improved by YGP and its disassembled formulas. The contents of CRH, ACTH and Cort were regulated by the above formulas. And the effect of YGP in the regulation of CRH and Cort was better than that of BY formula. Conclusion YGP and its disassembled formulas could improve the function of HPA axis in rats with kidney-yang deficiency. It might be related to the regulation of HPA axis. Therapeutical effect of YGP is better than the BY formula or TY formulas.

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备注/Memo

备注/Memo:
基金项目: 北京市自然科学基金(7132031); 北京市属高等学校高层次人才引进与培养计划—长城学者项目(CIT&TCD20140329)
作者简介: 刘浩龙(1988- ),2014级在读博士研究生。研究方向:天然药物学。E-mail: 793146134@qq.com
通信作者: 王蕾(1967- ),女,博士,教授,博士生导师。研究方向:中医药防治脑病研究。E-mail:tmwangl@ccmu.edu.cn
更新日期/Last Update: 2017-09-06