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[1]王怡悦 何侃 叶芊.葡萄籽原花青素预处理对幼龄小鼠深低温脑缺血/再灌注损伤的保护作用[J].环球中医药,2017,10(09):953-956.[doi:10.3969/j.issn.1674-1749.2017.09.005]
　WANG Yiyue,HE Kan,YE Qian..Protection of rape seed proanthocyanidin extract on cerebral ischemia-reperfusion induced by deep hypothermia in mice[J].,2017,10(09):953-956.[doi:10.3969/j.issn.1674-1749.2017.09.005]
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葡萄籽原花青素预处理对幼龄小鼠深低温脑缺血/再灌注损伤的保护作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年09期
页码:
953-956
栏目:
论著
出版日期:
2017-09-06

文章信息/Info

Title:
Protection of rape seed proanthocyanidin extract on cerebral ischemia-reperfusion induced by deep hypothermia in mice
作者:
王怡悦 何侃 叶芊
210023 南京特殊教育师范学院康复科学学院
Author(s):
WANG Yiyue;  HE Kan;  YE Qian.
Rehabilitation academy of sciences,Nanjing Normal University Of Special Education,Nanjing 210023,China
关键词:
葡萄籽原花青素;  脑缺血再灌注;  超氧化物歧化酶;  谷胱甘肽过氧化物酶;  丙二醛;  凋亡
Keywords:
Grape seed proanthocyanidin extract;  Cerebral ischemia-reperfusion;  Superoxide dismutase;  Glutathione peroxidase;  Malonyldialdehyde;  Apoptosis
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.09.005
文献标志码:
A
摘要:
目的 探讨葡萄籽原花青素(grape seed proanthocyanidin extract,GSPE)预处理对小鼠脑缺血/再灌注损伤保护作用机制。方法 120只3周龄C57/BL6小鼠随机分成3组:假手术组、模型组、GSPE组(每组再分为缺血120分钟再灌注后1、6、24、72小时,4个亚组,n=10),GSPE组灌胃给药2 mL/10 g(100 mg/kg),每天1次,共2周; 同时假手术组与模型组给予等量双蒸水。GSPE组于术前30分钟最后1次灌胃给药,模型组与假手术组则给予同体积双蒸水。分别于再灌注后1、6、24、72小时处死小鼠取脑,检测各组各时间点脑组织中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malonyldialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-px)含量,TUNEL法检测小鼠大脑皮层细胞凋亡。组间各时间点比较采用单因素方差分析和Bonferroni法两两比较。结果 GSPE组在再灌注后1、6、24小时SOD、GSH-px均明显高于模型组(P＜0.05),GSPE组MDA在1、6、24小时均高于假手术组(P＜0.05),但在6、24小时均低于模型组(P＜0.05); 与假手术组比较模型组和GSPE组于再灌注各时间点均出现明显的病理凋亡(P＜0.05),GSPE组各时间点病理凋亡均显著低于模型组(P＜0.05)。结论 GSPE可能通过诱导SOD、GSH-Px生成,清除MDA,对深低温缺血再灌注脑起保护作用。
Abstract:
Objective To determine the protection mechanism of Grape seed proanthocyanidin extract(GSPE)on rat model of cerebral ischemia/reperfusion(I/R)induced by deep hypothermic. Methods 120 three-week-old C57/BL6 mice were randomly divided into sham operation group,model group and GSPE group. Each group was redistributed into four subgroup of 1, 6, 24, 72h. The GSPE group was gavage with grape seed proanthocyanidin extract 2 ml/10 g(100 mg/kg), once a day for two weeks; while the sham group and model group was given the same amount of double-distilled water. The last intragastric administration were 30min before surgery, the model group and the sham group was treated with the same volume of double-distilled water. SOD, MDA, GSH-Px was detected in each time point, and the apoptotic level of cerebral cells was detected by Tunel staning. ANOVA and Bonferroni pairwise comparison method was used to compare each time point between the groups. Results SOD, GSH-px in GSPE group was significantly higher than those in the model group at different time points in reperfusion(P＜0.05), MDA in GSPE group were higher than that in the sham operation group(P＜0.05)in 1, 6, 24h, but lower than those in the model group in 6, 24h(P＜0.05); compared with the sham operation group, the pathologic apoptosis was significantly in reperfusion at each time point in model group and GSPE group(P＜0.05), compared with the model group, the pathologic apoptosis in GSPE group at each time point was alleviated(P＜0.05). Conclusion GSPE has a protective effect against deep hypothermic cerebral I/R injury through inducing SOD, GSH-Px and eliminating MDA.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介: 王怡悦(1985- ),女,硕士,助教,主治医师。研究方向:深低温缺血再灌注脑保护。E-mail:yiyuew@163.com
通信作者: 何侃(1963- ),女,本科,教授。研究方向:特殊儿童心理康复。E-mail:952729825@qq.com
更新日期/Last Update: 2017-09-06