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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第10卷

期数:

2017年09期

页码:

957-961

栏目:

论著

出版日期:

2017-09-06

文章信息/Info

Title:

Effects of Madai Tongluo decoction on lipid metabolism in apolipoprotein E-deficient mice

作者:

于少泓

250355 济南,山东中医药大学康复学院

Author(s):

YU Shaohong.

Rehabilitation College,Shandong University of Traditional Chinese Medicine,Jinan 250355,China

关键词:

马黛通络饮;  血脂;  载脂蛋白E基因敲除小鼠;  动脉粥样硬化;  脂质代谢

Keywords:

Madai Tongluo decoction;  Blood lipid;  ApoE -/- mice;  Atherosclerosis;  Lipid metabolism

分类号:

R285.5

DOI:

10.3969/j.issn.1674-1749.2017.09.006

文献标志码:

A

摘要:

目的 观察马黛通络饮对载脂蛋白E基因敲除小鼠脂质代谢的作用机制。方法 64只5周龄 ApoE-/-小鼠高脂饲料喂养,马黛通络饮灌胃干预8周后,检测小鼠血脂含量及主动脉的酯酰辅酶A-胆固醇酰基转移酶1(acy1-coenzyme A-cholesterol acyltransferases1,ACAT1)、氧化低密度脂蛋白的内皮受体(lectin-like OX-LDL receptor 1,LOX-1)的蛋白表达。结果 模型组血液中的总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)浓度明显高于正常组。与模型组相比较,马黛通络饮高剂量组明显降低血清TC、TG、LDL-C水平及主动脉组织中的ACAT1、LOX-1的表达,明显升高血清高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)水平(P＜0.05),低剂量组对TG、LDL-C、HDL-C的作用效果显著(P＜0.05),而对TC及ACAT1、LOX-1作用效果不明显(P＞0.05)。结论 马黛通络饮能降低ApoE-/-小鼠血脂水平,抑制ApoE-/-小鼠主动脉组织中的ACAT1、LOX-1的表达,通过调节血脂异常和脂质沉积发挥其阻止动脉粥样硬化及心脑血管疾病发生和发展的作用。

Abstract:

Objective To study the effect mechanism of Madai Tongluo decoction on vascular endothelial injury of atherosclerosis in apolipoprotein e-knockout mice.Methods Five-week-old male ApoE -/- mice were fed with a high fat high cholesterol diet. 8 weeks after the intervention of Madai Tongluo decoction. The level of serum lipid and the expression of acy1-coenzyme A-cholesterol acyltransferases1(ACAT1)、Lectin-like OX-LDL receptor 1(LOX-1)was detected. Results Serum contents of TC, TG and LDL-C in the Model group were significantly higher than that in normal group. Serum contents of TC, TG, LDL-C and the ACAT1、LOX-1 of aortic tissue in Madai Tongluo decoction high-dose group were significantly lower than those in model group(P＜0.05), and serum HDL-C in Madai Tongluo decoction high-dose group was significmitly lower than that in model group. TG、LDL-C、HDL-C and LOX-1 in Madai Tongluo decoction low-dose group were significant improved(P＜0.05), but TC and ACAT1 were non-significant(P＞0.05). Conclusions Madai Tongluo decoction can regulate lipid metabolism of ApoE -/- mice and inhibit ACAT1 and LOX-1 expression of aortic tissue. Madai Tongluo decoction can inhibit the occurrence and development of atherosclerotic lesions,cardiovascular and cerebrovascular diseases, the possible mechanisms may be associated with lipid metabolism and inhibition of lipid deposition.

参考文献/References:

[1] Arçari DP,Bartchewsky W,dos Santos TW,et al. Antiobesity effects of yerb mate extract(Ilex paraguariensis)in high-fat diet-induced obese mice[J]. Obesity,2009,17:2127-2133.
[2] Pang J,Choi Y,Park T. Ilex paraguariensisextract ameliorates obesity induced by hyperlipidemic diet,potential role of AMPK in the visceral adipose tissue[J]. Arch Biochem Biophys,2008,476:178-185.
[3] Bixby M,Spieler L,Menini T,et al. Ilex paraguariensis extracts are potent inhibitors of nitrosative stress,a comparative study with green tea and wines using a protein nitration model and mammalian cell cytotoxicity[J]. Life Sci,2005,77:345-358.
[4] Burris KP,Davidson PM,Stewart CNJr,et al. Antimicrobial activity of Yerba Mate(ilex paraguariensis)aqueous extracts against Escherichia coli O157,h7 and Staphylococcus aureus[J]. J Food Sci,2011,76:456-462.
[5] Puangpraphant S,Berhow MA,Vermillion K,et al. Dicaffeoylquinic acids in Yerba mate(Ilex paraguariensis St. Hilaire)inhibit NF-κB nucleus translocation in macrophages and induce apoptosis by activating caspases-8 and -3 in human colon cancer cells[J]. Mol Nutr Food Res,2011,55(10):1509-1522.
[6] Morais EC,Stefanuto A,Klein GA,et al. Consumption of yerba mate(Ilex paraguariensis)improves serum lipid parameters in healthy dyslipidemic subjects and provides an additional LDL holesterol reduction in individuals on statin therapy[J]. J Agr Food Chem,2009,57:8316-8324.
[7] Mosimann AL,Wilhelm-Filho D,Da Silva EL. Aqueous extract of Ilex paraguariensis attenuates the progression of atherosclerosis in cholesterol-fed rabbits[J]. Biofactors,2006,26:59-70.
[8] 于少泓.马黛多酚对动脉粥样硬化形成早期的干预机制研究[D].济南:山东大学,2015.
[9] 于少泓,吴俊玲,刘昭纯.马黛茶临床作用研究进展[J].山东中医药大学学报,2010,34(4):370-372.
[10] Yu Shaohong,Yue Shou Wei,Liu Zhaochun,et al. Yerba mate(Ilex paraguariensis)improves microcirculation of volunteers with high blood viscosity:A randomized,double-blind,placebo-controlled trial[J]. Experimental Gerontology,2015,(62):14-22.
[11] Tiwari RL,Singh V,Barthwal MK. Macrophages:an elusive yet emerging therapeutic target of atherosclerosis[J]. Med Res Rev,2008,28(4):483-544.
[12] Nissen SE,Tuzcu EM,Schoenhagen P,et al. Effect of intensive compared with moderate lipid-lowering therapy on progression of coronary atherosclerosis:a randomized controlled trial[J].JAMA,2004,291(9):1071-1080.
[13] Wissler RW. Update on the pathogenesis of atherosclerosis[J]. Am J Med,1991,91(1B):3S-9S.
[14] Koro CE,Bowlin SJ,Stump TE,et al. Low high-density lipoprotein cholesterol and cardiovascular disease[J].Am Heart J,2006,151(3):556-563.
[15] PN DuiTington,B-Mackness,MI Mackness. Paraoxonase and atherosclerosis[J].Arteriosclerosis,Thrombosis,and Vascular Biology,2001,21(4):473-480.
[16] Jovinge S,Hamsten A,Tomvall R,et al. Evidence for a role of tumor necrosis factor alpha in disturbances of triglyceride and glucose metabolism predisposing to coronary heart disease[J]. Metabolism,1998,47(1):113.
[17] Lei L,Xiong Y,Chen J,et al. TNF-alpha stimulates theACAT1 expression in differentiating monocytes to promote the CE-laden cell formation[J]. J Lipid Res,2009,50(6):1057-1067.

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备注/Memo

备注/Memo:

基金项目: 山东省高等学校科技计划(J16LK09); 山东省中医药科技发展计划(2011-022); 国家科技部国际合作项目(2010-016)
作者简介: 于少泓(1975- ),博士,副教授。研究方向:心脑血管疾病的康复研究。E-mail:sutcm2006@163.com

常用功能

更新日期/Last Update: 2017-09-06