|本期目录/Table of Contents|

[1]康学,许保海.参芪降糖颗粒对高糖环境下施万细胞氧化应激的调节作用[J].环球中医药,2017,10(11):1194-1197.[doi:10.3969/j.issn.1674-1749.2017.11.002]
　KANG Xue,XU Baohai..Effects of Shenqi Jiangtang granule on oxidative stressin Schwann cells with high glucose environment[J].,2017,10(11):1194-1197.[doi:10.3969/j.issn.1674-1749.2017.11.002]
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参芪降糖颗粒对高糖环境下施万细胞氧化应激的调节作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年11期
页码:
1194-1197
栏目:
论著
出版日期:
2017-11-06

文章信息/Info

Title:
Effects of Shenqi Jiangtang granule on oxidative stressin Schwann cells with high glucose environment
作者:
康学; 许保海
100035 北京积水潭医院中药房
Author(s):
KANG Xue;  XU Baohai.
Pharmacy of TCM, Beijing Jishuitan Hospital, Beijing 10035, China
关键词:
氧化应激;  施万细胞;  糖尿病周围神经病变;  参芪降糖颗粒
Keywords:
Oxidative stress;  Schwann cells;  Diabetic peripheral neuropathy;  Shenqi Jiangtang granule
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.11.002
文献标志码:
A
摘要:
目的 探讨参芪降糖颗粒对高糖环境下施万细胞氧化应激的调节作用。 方法 采用高糖环境下培养的施万细胞建立氧化应激模型,实验分为正常对照组、高糖组、参芪降糖含药血清组和甲钴胺含药血清组,干预48小时后,用酶标仪检测细胞中总抗氧化能力(total antioxidant capacity,T-AOC)、超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde, MDA)含量,应用流式细胞仪检测活性氧分子(reactive oxygen species,ROS)水平。 结果 高糖干预48小时后,与正常对照组比较,高糖组细胞T-AOC和SOD含量明显降低,ROS、MDA水平显著升高(P<0.01); 与高糖组比较,参芪降糖含药血清能显著提高细胞T-AOC和SOD含量,明显下调ROS和MDA水平(P<0.05)。 结论 参芪降糖颗粒能够明显提高具有抗氧化能力物质T-AOC和SOD水平,清除有害的氧化代谢产物ROS和MDA,通过抑制高糖环境下施万细胞的氧化应激而发挥对糖尿病周围神经病变的保护作用。
Abstract:
Objective To explore the effects of Shenqi Jiangtang granule on oxidative stress in Schwann cells with high glucose environment. Methods Oxidative stress model of Schwann cells was established by high glucose environment. The experiment was divided into normal control group, high glucose group, Shenqi Jiangtang serum group and methylcobalamin serum group and treatment for 48 hours(h). Microplate Reader was used to detect the content of total antioxidant capacity(T-AOC), superoxide dismutase(SOD)and malondialdehyde(MDA). Flow cytometry was used to analysis the level of reactive oxygen species(ROS). Results After incubated with high glucose for 24 h, the content of T-AOC and SOD in high glucose group were significantly lower, while ROS and MDA activity were significantly higher than normal control group(P<0.01). Compared with high glucose group, Shenqi Jiangtang serum could significantly increase the content of T-AOC and SOD, while the level of ROS and MDA was decreased obviously(P<0.05). Conclusion Shenqi Jiangtang granule can obviously improve the levels of antioxidant substances T-AOC and SOD, meanwhile eliminate the harmful oxidation metabolites ROS and MDA, play a protective effect on diabetic peripheral neuropathy by inhibiting oxidative stress in Schwann cells with high glucose environment.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介: 康学(1989- ),女,硕士,药师。研究方向:中药药效与作用机制。E-mail:kangxue0907@126.com
通信作者: 许保海(1970- ),本科,主任药师。研究方向:中药调剂、鉴定,合理使用中成药。E-mail:xubaohai70@163.com
更新日期/Last Update: 2017-11-06