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[1]焦海燕,严志祎,姜幼明,等.慢性束缚应激抑郁症大鼠模型的复制及逍遥散对海马色氨酸代谢途径的调节作用[J].环球中医药,2017,10(11):1198-1202.[doi:10.3969/j.issn.1674-1749.2017.11.003]
　JIAO Haiyan,YAN Zhiyi,JIANG Youming,et al.Model of depression rats with chronic immobilization stress and effects of Xiaoyaosan on the change of tryptophan metabolism pathway[J].,2017,10(11):1198-1202.[doi:10.3969/j.issn.1674-1749.2017.11.003]
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慢性束缚应激抑郁症大鼠模型的复制及逍遥散对海马色氨酸代谢途径的调节作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年11期
页码:
1198-1202
栏目:
论著
出版日期:
2017-11-06

文章信息/Info

Title:
Model of depression rats with chronic immobilization stress and effects of Xiaoyaosan on the change of tryptophan metabolism pathway
作者:
焦海燕; 严志祎; 姜幼明; 刘玥芸; 陈家旭
100029 北京中医药大学中医学院[焦海燕(硕士研究生)、严志祎(博士研究生)、姜幼明、刘玥芸、陈家旭]
Author(s):
JIAO Haiyan; YAN Zhiyi; JIANG Youming; et al.
Institute of TCM, Beijing University of Chinese Medicine,Beijing 100029,China
关键词:
抑郁症;  色氨酸代谢途径;  5-羟色胺;  逍遥散
Keywords:
Depression;  Tryptophan metabolism pathway;  5-HT;  Xiaoyaosan
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.11.003
文献标志码:
A
摘要:
目的 通过慢性束缚应激方法复制大鼠抑郁症模型,并观察海马色氨酸代谢途径的变化与逍遥散的调节作用。方法 将24只雄性SD大鼠随机分成正常组、模型组、逍遥散组与氟西汀组。采用21天慢性束缚应激的方法复制大鼠抑郁症模型,通过大鼠体重变化、糖水偏好实验、新环境抑制进食实验、旷场试验对模型进行评价。采用ELISA法测定各组大鼠海马色氨酸(tryptophan,Trp)与5-羟色胺(5-HT)的含量。结果 模型组大鼠糖水偏好量和新环境进食时间与正常组大鼠相比有显著差异(P<0.01); 旷场试验的中央区进入次数、中央区停留时间以及总穿格次数与正常组相比均有统计学差异(P<0.01,P<0.05)。ELISA结果显示,模型组大鼠海马5-HT含量显著低于正常组(P<0.01),而逍遥散组和氟西汀组海马5-HT含量明显高于模型组(P<0.05,P<0.01); 模型组大鼠海马中色氨酸的含量较正常组明显增加(P<0.01)。结论 在慢性束缚应激抑郁症大鼠海马中存在色氨酸代谢途径的异常,这与抑郁症的发病密切相关,且逍遥散能够通过对海马色氨酸代谢的调节起到一定的抗抑郁作用。
Abstract:
Objective To establish the depression rat model by chronic immobilization stress method,and observe the change of tryptophan metabolism pathway in hippocampus and the regulation of Xiaoyaosan. Methods 24 SD rats were randomly divided into normal group,model group,Xiaoyaosan group and fluoxetine group,the 21 days depression rat model was made by chronic immobilization stress method,and the model was evaluated by bodyweight change,sucrose preference test,novelty suppressed feeding test and open field test. ELISA method was used to determine the contents of tryptophan and 5-HT in hippocampus. Results The results of sucrose preference test and novelty suppressed feeding test showed that there was a significant difference between model group and normal group(P<0.01),the central enterance,central residence and total cross number of rats in model group were significantly different compared with that of normal group(P<0.01,P<0.05). The ELISA results showed that the content of 5-HT in hippocampus of rats in model group was significantly lower than that of normal group(P<0.01),the contents of 5-HT of rats in Xiaoyaosan group and fluoxetine group were significantly higher than that of model group(P<0.05,P<0.01); the content of tryptophan in model group was significantly higher than normal group(P<0.01). Conclusion There existed abnormity in the tryptophan metabolic pathway of depression model rat by chronic immobilization stress,which was closely related to the incidence of depression,and Xiaoyaosan could possess antidepressant effect by regulating the tryptophan metabolism in hippocampus.

参考文献/References:

[1] 李那永. 抑郁症中医证候要素及其分布特征研究[D]. 北京:北京中医药大学,2013.
[2] 郭保全,石长珍. 老年抑郁症治疗体会[J]. 国医论坛,2004,19(4):28.
[3] Willner P,Towell A,Sampson D,et al. Reduction of sucrose preference by chronic unpredictable mild stress,and its restoration by a tricyclic antidepressant[J]. Psychopharmacology,1987,93(3):358-364.
[4] Shalom G,Lifschytz T,Lerer B,et al. Effects of T3 and fluoxetine in the novelty-suppressed feeding test model of depression and anxiety: gender differences[J]. European Neuropsychopharmacology,2006,16(6):S48-S49.
[5] Hallam KT,Horgan JC,Norman TR. An investigation of the effect of tacrine and physostigmine on spatial working memory deficits in the olfactory bulbectomised rat[J]. Behavioural Brain Research,2004,153(2):481-486.
[6] Castern E. Is mood chemistry[J]. NatRevNeuorsei,2005,6(3):241-246.
[7] Varea V,de Carpi J M,Puig C,et al. Malabsorption of carbohydrates and depression in children and adolescents[J]. J Pediatr Gastroenterol Nutr,2005,40(5):561-565.
[8] Korzan W J,Summers C H. Serotonergic response to social stress and artificial social sign stimuli during paired interactions between male Anolis carolinensis[J].Neuroscience,2004,123(4):835-845.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金面上项目(81473597); 国家自然科学基金重点项目(81630104); 北京中医药大学2016年校级自主课题(2016-JYB-XS012)
作者简介: 焦海燕(1991- ),女,2014级在读硕士研究生。研究方向:证候的生物学基础。E-mail:2548489775@qq.com
通信作者: 陈家旭(1966- ),博士,教授,博士生导师。研究方向:证候的生物学基础。E-mail:chenjx@bucm.edu.cn
更新日期/Last Update: 2017-11-06