|本期目录/Table of Contents|

[1]赵唯含,史瑞,谭祥,等.益气活血法对MNNG诱导萎缩性胃炎癌前病变大鼠hedgehog信号通路的调控作用[J].环球中医药,2017,10(11):1207-1212.[doi:10.3969/j.issn.1674-1749.2017.11.005]
　ZHAO Weihan,SHI Rui,TAN Xiang,et al.Regulation effects of invigorating qi and activating blood therapy on the hedgehog signal pathway in rats with precancerous lesions of atrophic gastritis[J].,2017,10(11):1207-1212.[doi:10.3969/j.issn.1674-1749.2017.11.005]
点击复制

益气活血法对MNNG诱导萎缩性胃炎癌前病变大鼠hedgehog信号通路的调控作用()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年11期
页码:
1207-1212
栏目:
论著
出版日期:
2017-11-06

文章信息/Info

Title:
Regulation effects of invigorating qi and activating blood therapy on the hedgehog signal pathway in rats with precancerous lesions of atrophic gastritis
作者:
赵唯含; 史瑞; 谭祥; 陈晨; 毛堂友; 李军祥
712000 陕西中医药大学附属医院消化一科(赵唯含); 北京中医药大学东方医院消化内科[史瑞、谭祥(硕士研究生)、陈晨(硕士研究生)、毛堂友(博士研究生)、李军祥]
Author(s):
ZHAO Weihan; SHI Rui; TAN Xiang; et al.
Department of Gastroenterology,the hospital of Shanxi University of Chinese Medicine,Shanxi 712000,China
关键词:
萎缩性胃炎癌前病变;  益气活血法;  hedgehog信号通路;  黄芪;  三七
Keywords:
Precancerous lesions of atrophic gastritis;  Invigorating qi and activating blood therapy;  Hedgehog signal pathway;  Astragalus;  Panax notoginseng
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.11.005
文献标志码:
A
摘要:
目的 探讨益气活血法对萎缩性胃炎癌前病变大鼠hedgehog信号通路的调控影响。方法 雄性Wistar大鼠随机分为空白组、模型组、黄芪组、三七组、黄芪三七组、Purmorphamine组、环巴明组及叶酸组,运用MNNG负荷多因素法制备萎缩性胃炎癌前病变模型。应用HE染色评价胃黏膜病理损伤,应用Western Blot、RT-PCR和量子点介导的免疫荧光化学法分别检测各组大鼠胃组织hedgehog信号通路的蛋白和基因表达水平。结果 与空白组相比,模型组大鼠血清PGⅠ/PGⅡ和GAS含量显著降低(P<0.01),Shh、Ptch基因及蛋白含量均明显下调(P<0.05,P<0.01),Smo蛋白表达减弱(P<0.01)。与模型组相比,三七组、黄芪三七组及Purmorphamine组PGⅠ/PGⅡ明显升高(P<0.01),黄芪组、三七组及黄芪三七组GAS含量显著升高(P<0.05)。三七组、黄芪三七组及叶酸组Shh蛋白表达明显升高(P<0.05),三七组和Purmorphamine组Ptch蛋白表达增多(P<0.05),黄芪组、三七组、黄芪三七组和Purmorphamine组Smo蛋白表达显著增强(P<0.05,P<0.01)。结论 益气活血法可激活hedgehog信号通路,对萎缩性胃炎癌前病变大鼠胃黏膜病变起到改善作用。
Abstract:
Objective To evaluate the effects of invigorating qi and activating blood therapy on hedgehog signal pathway in rats with precancerous lesions of atrophic gastritis. Methods The model was established by MNNG, male wistar rats were randomly divided into control group, model group, Astragalus group, Panax notoginseng group, Astragalus plus Panax notoginseng group, Purmorphamine group, cyclopamine group and folic acid group. HE was used to evaluate the pathological injury of stomach. The mRNA and protein expression levels of hedgehog signal pathway were detected by RT-PCR, Western Blot analysis and immunofluorescence. Results Compared with the control group, GAS and PG I /PG II level were reduced in model group(P<0.01), the mRNA and protein expression levels of Shh and Ptch were obviously down-regulated(P<0.05,P<0.01); the expression of Smo protein was decreased(P<0.01). Compared with the model group, the level of PG I /PG II was increased in the Panax notoginseng group, Astragalus plus Panax notoginseng group and Purmorphamine group(P<0.01), the level of GAS was increased in the Astragalus group, Panax notoginseng group and Astragalus plus Panax notoginseng group(P<0.05). The protein of Shh was increased in the Panax notoginseng group, Astragalus plus Panax notoginseng group and folic acid group(P<0.05). The protein of Ptch was markedly up-regulated in Panax notoginseng group and Purmorphamine group(P<0.05). The expression of Smo was significantly increased in Astragalus group, Panax notoginseng group and Astragalus plus Panax notoginseng group and Purmorphamine group(P<0.05,P<0.01).Conclusion Invigorating qi and activating blood therapy can activate hedgehog signal pathway, and can improve the gastric mucosal lesion in precancerous lesion of atrophic gastritis.

参考文献/References:

[1] 杨印智,王继栓,王晓琴,等.慢性萎缩性胃炎的诊断与中医药治疗[J].现代中西医结合杂志,2014,23(24):2686-2687.
[2] Correa P, Shiao YH.Phenotypic and genomic events in gastric carcinogenesis[J]. Cancer Res, 1994,5(4):1941.
[3] 郑保平.慢性萎缩性胃炎及其癌前病变的病机和证治探讨[J].江苏中医药,2010,42(1):8-9.
[4] 李俊青.参七消痞颗粒对MNNG负荷多因素致大鼠慢性萎缩性胃炎的干预作用及机制探讨[D].北京:北京中医药大学,2013.
[5] 赵晓丹,孙静晶,周斌.慢性萎缩性胃炎中医证型分布规律[J].环球中医药,2015,8(1):18-21.
[6] 李军祥,张玉禄,朱陵群,等.益气活血法对大鼠萎缩性胃炎癌前病变差异基因表达谱的影响[J].世界华人消化杂志,2008,16(29):3261-3268.
[7] Martin J, Donnelly JM, Houghton J, et al.The role of sonic hedgehog reemergence during gastric cancer[J].Dig Dis Sci, 2010, 55(6):1516-1524.
[8] 赵唯含,高康丽,李宁飞,等. 黄芪、三七及其配伍对慢性萎缩性胃炎大鼠胃组织Hedgehog信号通路的调节作用[J]. 中华中医药杂志,2016,31(5):1951-1955.
[9] shiotani. S evidence that loss of sonic hedgehog is an indicator of an helicopter pylori-induced atrophic gastritis progressing to gastric cancer[J]. AM.J.Gastroenterol.2005, 100(3):581-587.
[10] Bijlsma MF, Spek CA, Zivkovic D, et al. Repression of smoothened by patched-dependent(pro-)vitamin D3 secretion[J]. PLoS Biol,2006,4(8): e232.
[11] 俞卫宁,俞萍.叶酸、维生素B1、吗丁啉联合治疗萎缩性胃炎疗效观察[J].中国社区医师,2011,13(25):75.
[12] Faghihi F, Baghaban Eslaminejad M, Nekookar A, et al. The effect of purmorphamine and sirolimus on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells[J]. Biomed Pharmacother, 2013, 67(1):31-38.
[13] Wu JY, Xu XF, Xu L,et al. Cyclopamine blocked the growth of colorectal cancer SW116 cells by modulating some target genes of Gli1 in vitro[J]. Hepatogastroenterology, 2011,(58):1511-1518.
[14] Martin J, Donnelly JM, Houghton J, et al.The role of sonic hedgehog reemergence during gastric cancer[J].Dig Dis Sci, 2010, 55(6):1516-1524.

相似文献/References:

[1]李晓红,胡立明,裴文婧,等.中医辨证治疗萎缩性胃炎癌前病变59例[J].环球中医药,2016,9(12):1541.[doi:10.3969/j.issn.1674-1749.2016.12.035]

备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81373585)
作者简介: 赵唯含(1988- ),女,博士,讲师。研究方向:中医药防治胃肠疾病的研究。E-mail: weihanzhaodoc@163.com
通信作者: 李军祥(1964- ),博士,主任医师,博士生导师。研究方向: 中医药防治慢性肝胆和胃肠道疾病。E-mail:lijunxiang1226@163.com
更新日期/Last Update: 2017-11-06