|本期目录/Table of Contents|

[1]任攀,康群甫,周明学,等.当归芍药散对动脉粥样硬化小鼠的干预作用及DNA甲基转移酶1和PPAR-γ表达的影响[J].环球中医药,2017,10(11):1228-1232.[doi:10.3969/j.issn.1674-1749.2017.11.009]
　REN Pan,KANG Qunfu,ZHOU Mingxue,et al.Effects of Danggui Shaoyao powder on the DNMT1 and the expression of PPARγ in atherosclerotic mice[J].,2017,10(11):1228-1232.[doi:10.3969/j.issn.1674-1749.2017.11.009]
点击复制

当归芍药散对动脉粥样硬化小鼠的干预作用及DNA甲基转移酶1和PPAR-γ表达的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年11期
页码:
1228-1232
栏目:
论著
出版日期:
2017-11-06

文章信息/Info

Title:
Effects of Danggui Shaoyao powder on the DNMT1 and the expression of PPARγ in atherosclerotic mice
作者:
任攀; 康群甫; 周明学; 张蕾; 李思耐; 刘卫红
100010 北京,首都医科大学附属北京中医医院北京市中医研究所[任攀(硕士研究生)、康群甫(硕士研究生)、周明学、张蕾、李思耐、刘卫红]
Author(s):
REN Pan; KANG Qunfu; ZHOU Mingxue; et al.
Beijing Institute of Traditional Chinese Medicine,Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing 100010,China
关键词:
动脉粥样硬化;  当归芍药散;  DNA甲基化;  DNA甲基化转移酶;  DNA甲基转移酶1;  过氧化物酶体增殖物激活受体γ
Keywords:
Atherosclerosis;  Danggui Shaoyao powder;  DNA methylation;  DNA methyltransferase;  DNA methyltransferase 1;  Peroxisome proliferator activated receptor gamma
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.11.009
文献标志码:
A
摘要:
目的 研究当归芍药散对载脂蛋白E基因敲除(ApoE-/-)小鼠主动脉粥样硬化(atherosclerosis, As)斑块的干预作用及DNA甲基转移酶1(DNMT1)和PPAR-γ表达的影响。方法 将30只8周龄雄性ApoE-/-小鼠西方类型膳食喂养9周,随机分为模型组、立普妥组(阳性对照组)和当归芍药散组(n=10),药物干预9周后,检测血清总胆固醇(total cholesterol, TC)、甘油三酯(triglyceride, TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、血清DNA甲基化水平及DNMTs水平。行苏木素-伊红染色(hematoxylin and eosin staining, HE),采用IPP图像分析软件测量小鼠主动脉As斑块面积。免疫组化法检测各组小鼠主动脉As斑块内DNA甲基化转移酶1(DNMT1)和过氧化物酶体增殖物活化受体γ(PPAR γ)的表达。结果 与模型组相比,当归芍药散组和立普妥组小鼠血清TC、TG水平明显降低(P<0.05),当归芍药散组小鼠血清HDL-C水平明显升高(P<0.05)。与模型组相比,当归芍药散组小鼠主动脉As斑块面积显著降低(P<0.01),当归芍药散组小鼠主动脉斑块内DNMT1表达显著降低(P<0.05),PPAR γ表达显著升高(P<0.05)。结论 当归芍药散可改善As小鼠的血脂水平,减少As斑块面积,具有明确的抗As作用,其机制可能与抑制As小鼠血清甲基化水平和DNMTs 水平,促进As小鼠主动脉斑块内PPARγ表达和抑制斑块内DNMT1表达有关。
Abstract:
Objective To study the effects of Danggui Shaoyao powder on the expression of DNA methyltransferase 1(DNMT1)and PPARγ in apolipoprotein E knockout(ApoE-/-)mice with atherosclerosis. Methods Thirty 8 week-old male ApoE-/- mice were fed with high fat diet for 9 weeks, and the mice were randomly divided into the control group, the Lipitor(positive control group), the Danggui Shaoyao powder group(n=10). The levels of total cholesterol(TC), triglycerides(TG), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C)in serum were detected. The level of DNA Methylation and the DNMTs in serum were detected. HE staining was used to measure the area of atherosclerosis plaque of mice. Immuno-histochemical staining and image analysis were used to measure the expressions of DNMT1 and PPARγ in the atherosclerosis plaques of mice. Results Compared with the model group, the TC and TG levels of Danggui Shaoyao powder group and Lipitor group were significantly decreased(P<0.05), the HDL-C levels of Danggui Shaoyao powder group were significantly increased(P<0.05). Compared with the model group, the aorta As plaque area was significantly decreased in Danggui Shaoyao powder group(P<0.05). The expressions of DNMT1 of the Danggui Shaoyao powder group were significantly reduced(P<0.05)while the expressions of PPARγ were significantly increased(P<0.05). Conclusion Danggui Shaoyao powder can improve the blood lipid level and the plaque area of As mice. The mechanism may be related to the reduction of DNA methylation level and the DNMTs level in serum, and also related to the inhibition of DNMT1 expression, and the promotion of PPARγ expression in mice with As.

参考文献/References:

[1] 刘俊田.As发病的炎症机制的研究进展[J].西安交通大学学报(医学版),2015,36(2):141-152.
[2] 谭剑凯,谭小进,王佐.DNA甲基化与As[J].中国生物化学与分子生物学报,2014,30(2):132-136.
[3] Khyzha N,Alizada A,Wilson MD,et al. Epigenetics of Atherosclerosis:Emerging Mechanisms and Methods[J]. Trends Mol Med,2017,23(4):332-347.
[4] Yu J,Qiu Y,Yang J,et al. DNMT1-PPARγ pathway in macrophages regulates chronic inflammation and atherosclerosis development in mice[J]. Sci Rep,2016,(17):30053.
[5] 宋晓宇,张天真,徐薇,等.当归芍药散治疗血脂异常痰瘀互结证的临床观察[J].河北中医报,2009,24(1):12-13.
[6] 陈静,叶平,阎艳丽,等.当归芍药散防治高脂血症的实验研究[J].天津中医药,2005,22(6):493-494.
[7] 阎艳丽,吉梅,宋晓宇,等.当归芍药散对血脂异常大鼠抗氧化能力及动脉壁血管细胞黏附分子-1基因表达的影响[J].中国实验方剂学杂志,2007,13(2):25-28.
[8] 贾丽超,周明学,张蕾,等. 当归芍药散对代谢性炎性反应小鼠血脂和血清炎性反应因子IL-6、MCP-1及NF-κB、PPARγ、mRNA表达的影响[J].首都医科大学学报,2014,35(6):813-817.
[9] 董培良,殷鑫,张天宇,等.当归芍药散对实验性高脂血症模型大鼠的影响(1)[J].中医药学报,2013,41(6):81-83.
[10] XR Yang,E Wat,YP Wang,et al. Effect of Dietary Cocoa Tea(Camellia ptilophylla)Supplementation on High-fat Diet-Induced Obesity,Hepatic Steatosis and Hyperlipidemia in Mice[J].Evidence-BASed Complementary and Alternative Medicine,2013,(3),783-860.
[11] Hiltunen MO,Turunen MP,Häkkinen TP,et al. DNA hypomethylation and methyltransferAse expression in atherosclerotic lesions[J]. VAsc Med,2002,7(1):5-11.
[12] Revankar C M,Cimino D F,Sklar L A,et al. A transmembrane intracellular estrogen receptor mediates rapid cell signaling[J].Science,2005,307(5715):1625-1630.
[13] Post W S,Goldschmidt-Clermont P J,Wilhide C C,et al. Methylation of the estrogen receptor gene is Associated with aging and atherosclerosis in the cardiovAscular system[J].CardiovAsc Res,1999,43(4):985-991.
[14] Kim M,Long TI,Arakawa K,et al. DNA methylationAsa biomarker for cardiovAscular diseAse risk[J].PLoS One,2010,5(3):e9692.
[15] Valinluek V,Sowers LC. Endogenous cytosine damage products alter the site selectivity of human DNA maintenance methyltransferAse DNMT1[J].Caneer Res,2007,67(3):946-950.
[16] 陈晓娟,闫少春,邵国. 人DNA甲基化转移酶的分类及其功能[J]. 包头医学院学报,2014,30(4):136-138.
[17] 吴圣贤,聂波,潘美香,等. 解毒活血法对ApoE-/-小鼠As斑块PPAR-γ和炎性因子的影响[J].辽宁中医杂志,2014,41(5):1046-1048.
[18] 赵静. 青钱柳多糖对大鼠脂代谢相关基因DNA甲基化及表达影响的研究[D].南昌:江西农业大学,2016.
[19] 范锐心.甜菜碱对apoE基因缺陷小鼠AS发生及TNF-α甲基化的影响[D].广州:中山大学,2007.

相似文献/References:

[1]乌兰其其格 麻春杰 呼日乐巴根等.额尔敦—乌日勒对兔动脉粥样化易损斑块模型血清超敏C反应蛋白 及相关炎症因子的影响[J].环球中医药,2014,7(03):183.
　Wulanqiqige,MA Chun jie,Hurilebagen,et al.Influences of serum hsCRP and relevant inflammatory factors on rabbit atherosclerotic vulnerable plaque model with Eerdunwurili[J].,2014,7(11):183.
[2]李艳阳 吕仕超 仲爱芹 张军平.从脾肾论治动脉粥样硬化[J].环球中医药,2014,7(03):204.
[3]王椿野,赵振武,李新龙,等.基于现代文献的动脉粥样硬化中医病机研究[J].环球中医药,2013,6(02):92.
　WANG Chun ye,ZHAO Zhen wu,LI Xin long,et al.Distribution of TCM syndrome factors of atherosclerosis based on modern literature[J].,2013,6(11):92.
[4]张国侠,盖国忠.莱菔子水溶性生物碱对载脂蛋白E基因缺陷小鼠胸主动脉内皮型一氧化氮合酶、核因子-кB的影响[J].环球中医药,2013,6(08):570.
　ZHANG Guo xia,GAI Guo zhong..Experimental study of thoracic aorta eNOS，NFκB effect of soluble Semen Raphani alkaloids in ApoE/ Rats[J].,2013,6(11):570.
[5]张颖 周明学 李思耐 任攀 刘卫红 尚菊菊 刘红旭.参元丹胶囊对动脉粥样硬化小鼠主动脉斑块的干预作用及胰岛素抵抗标志蛋白GLUT-4的影响[J].环球中医药,2017,10(07):677.[doi:10.3969/j.issn.1674-1749.2017.07.001]
　ZHANG Ying,ZHOU Mingxue,LI Sinai,et al.Effects of Shenyuandan capsule on atherosclerotic plaque and the expression of GLUT-4 in atherosclerotic mice[J].,2017,10(11):677.[doi:10.3969/j.issn.1674-1749.2017.07.001]

备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81573735); 北京市自然科学基金(7142037)
作者简介: 任攀(1990- ),2015级在读硕士研究生。研究方向:中西医结合心血管病研究。E-mail:renpanzy@163.com
通信作者: 刘卫红(1968- ),女,博士,硕士生导师,主任医师。研究方向:中医药防治心血管病的疗效评价及作用机制研究。 E-mail:wh.l-007@163.com
更新日期/Last Update: 2017-11-06