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[1]孙凯,张平,李永秀,等.益肾解毒方对肺腺癌骨转移裸鼠JNK/Bcl-2信号传导通路的影响[J].环球中医药,2017,10(11):1233-1238.[doi:10.3969/j.issn.1674-1749.2017.11.010]
　SUN Kai,ZHANG Ping,LI Yongxiu,et al.Effects of Yishen Jiedu decoction on JNK / Bcl-2 signaling pathway in nude mice with bone metastasis of lung adenocarcinoma[J].,2017,10(11):1233-1238.[doi:10.3969/j.issn.1674-1749.2017.11.010]
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益肾解毒方对肺腺癌骨转移裸鼠JNK/Bcl-2信号传导通路的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年11期
页码:
1233-1238
栏目:
论著
出版日期:
2017-11-06

文章信息/Info

Title:
Effects of Yishen Jiedu decoction on JNK / Bcl-2 signaling pathway in nude mice with bone metastasis of lung adenocarcinoma
作者:
孙凯; 张平; 李永秀; 胡佩岩; 陈冠敏
100102 北京,中国中医科学院望京医院病理科[孙凯(硕士研究生)、张平、李永秀(硕士研究生)、陈冠敏(硕士研究生)],关节科(胡佩岩)
Author(s):
SUN Kai; ZHANG Ping; LI Yongxiu; et al.
Department of pathology,Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102,China
关键词:
益肾解毒方;  c-Jun氨基末端激酶;  B淋巴细胞瘤-2;  B淋巴细胞瘤-2相关的X蛋白;  天冬氨酸特异性半胱氨酸蛋白酶-3;  肺腺癌骨转移
Keywords:
Yishen Jiedu decoction;  JNK;  Bcl-2;  Bax;  Caspase-3;  Lung adenocarcinoma bone metastasis
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.11.010
文献标志码:
A
摘要:
目的 探讨益肾解毒方对肺腺癌骨转移裸鼠JNK/Bcl-2信号传导通路的影响。方法 本研究在前期成功建立的裸鼠肺腺癌转移模型的基础上,运用免疫组织化学染色法检测各组JNK、Bcl-2、Bax、Caspase-3蛋白的表达,图像分析使用MIA 4.0图像分析软件,观察和测量显微镜400倍视野下表达的阳性物质的光密度值。结果 模型组与益肾解毒方高剂量组JNK蛋白的表达最低,两者无统计学差异(P>0.05); 帕米磷酸二钠组JNK蛋白的表达最高,低剂量组、中剂量组及中+西药组次之,与模型组和高剂量组相比都有统计学差异(P<0.05); 模型组Bcl-2蛋白的表达最高,其次是益肾解毒方高剂量组、中剂量组、帕米磷酸二钠组,Bcl-2蛋白表达最低的是低剂量组与中+西药组,与模型组比较得出,各实验组Bcl-2蛋白表达显著降低,差异有统计学意义(P<0.05); 模型组Bax蛋白表达最低,与其他实验组比较,差异有统计学意义(P<0.05),中+西药组Bax蛋白表达最高,其次是帕米磷酸二钠组、低剂量组、高剂量组、中剂量组; 益肾解毒方高剂量组与中剂量组比较,差异无统计学意义(P>0.05); 益肾解毒方低剂量组Caspase-3蛋白表达最高,其次是中剂量组、中+西药组、帕米磷酸二钠组,益肾解毒方高剂量组和模型组Caspase-3蛋白表达最低; 与模型组相比,除高剂量组外其余各实验组均有统计学差异(P<0.05)。结论 肺腺癌骨转移与JNK/Bcl-2信号传导通路密切相关。本实验结果表明益肾解毒方对该信号通路上的关键分子JNK、Bcl-2、Bax 及Caspase-3蛋白有明显的调控作用,证实了这可能是其促进肿瘤细胞凋亡的关键环节,为中医药临床有效防治恶性肿瘤提供了一定的理论依据。
Abstract:
Objective To investigate the effects of Yishen Jiedu decoction on JNK / Bcl-2 signal transduction pathway in nude mice with bone metastasis of lung adenocarcinoma. Methods The expression of JNK, Bcl-2, Bax and Caspase-3 protein in each group was detected by immunohistochemical staining on the basis of the established model of lung adenocarcinoma in nude mice. The image analysis was performed by MIA 4.0 Software to observe and measure the optical density of the positive substance expressed in the 400-fold field of view of the microscope. Results The expression of JNK protein in model group and Yishen Jiedu high group was the lowest, and there was no significant difference between them(P>0.05). The expression of JNK protein was the highest in Pamidium phosphate disodium group,the expression of JNK protein in low dose group, middle dose group and Chinese medicine + western medicine group was next, and compared with model group and high dose group, there was significant difference(P<0.05). The expression of Bcl-2 protein in the model group was the highest, the high dose group,the middle dose group and Pamidium phosphate disodium group was followed, the low dose group and the Chinese medicine + western medicine group was the lowest. The expression of Bcl-2 protein in each experimental group was significantly lower than that in the model group(P<0.05). The expression of Bax protein was the lowest in the model group, and the difference was statistically significant compared with other experimental groups(P<0.05). The expression of Bax protein was the highest in the Chinese medicine + western medicine group, the disodium group of pamidronate, low dose group, high dose group, middle dose group was followed. There was no significant difference between the high dose group and the middle dose group(P>0.05). The expression of Caspase-3 protein was the highest in the low dose group, the middle dose group, the Chinese medicine + western medicine group, the pamidium phosphate disodium group was followed. The expression of Caspase-3 protein was the lowest in the high dose group and the model group. Compared with the model group, there were significant differences between the other experimental groups except the high dose group(P<0.05).Conclusion Bone metastasis of lung adenocarcinoma is closely related to JNK / Bcl-2 signaling pathway. The results of this study show that Yishen Jiedu decoction can regulate JNK, Bcl-2, Bax and Caspase-3 proteins in this signal pathway, which may be a key step in promoting tumor cell apoptosis and provides a theoretical basis for the effective prevention and treatment of malignant tumors.

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备注/Memo

备注/Memo:
基金项目: 中国中医科学院中央级公益性科研院所科研基本业务费自主选题(ZZ070805)
作者简介: 孙凯(1991- ),2015级在读硕士研究生。研究方向:中西医结合肿瘤的诊断与治疗研究。E-mail:1163262599@qq.com
通信作者: 张平(1973- ),女,博士,主任医师,硕士生导师。研究方向:中西医结合防治肿瘤疾病的临床和基础研究。E-mail:pinglele@sina.com
更新日期/Last Update: 2017-11-06