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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第10卷

期数:

2017年12期

页码:

1417-1423

栏目:

论著

出版日期:

2017-12-06

文章信息/Info

Title:

Effects of acupuncture at Renying(ST9)on gene expression profile of hypothalamus in spontaneously hypertensive rats

作者:

郭妍; 石学敏; 赵瑞利; 芦娟; 马田

300192 天津中医药大学第一附属医院(郭妍、石学敏); 北京中医药大学针灸推拿学院[赵瑞利(硕士研究生)、芦娟(博士研究生)、马田(硕士研究生)]

Author(s):

GUO Yan;  SHI Xuemin;  ZHAO Ruili; et al.

The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300192, China

关键词:

针刺;  人迎穴;  原发性高血压;  下丘脑;  基因芯片

Keywords:

Acupuncture;  Renying point(ST9);  Primary hypertensive;  Hypothalamus;  Gene chip

分类号:

R245.3

DOI:

10.3969/j.issn.1674-1749.2017.12.001

文献标志码:

A

摘要:

目的 本实验从基因芯片层面探讨针刺人迎穴对自发性高血压大鼠(spontaneous hypertensive rats,SHR)下丘脑基因表达的效应差异。 方法 将20只SHR随机分为人迎组和模型组,另取10只WKY大鼠作为空白对照组。人迎组每日针刺人迎穴并施以捻转泻法,模型组和空白组不进行针刺干预,并对大鼠每四天测量血压一次,实验共28天。结束后取材进行基因芯片分析。 结果 空白组血压无明显变化,模型组血压与空白组相比显著升高(P<0.01); 人迎组血压虽未降至正常,但与模型组相比,差异具有统计学意义(P<0.01)。芯片结果显示:不同组别之间显示出基因表达的差异性。与空白组相比,模型组的数据分析显示有129个基因上调,243个基因下调; 与模型组比较,人迎组有167个基因上调,188个基因下调。 结论 针刺人迎穴对SHR有明显的降压效应,对大鼠下丘脑的基因表达有一定影响,其机制可能与针灸抗高血压原理有关。

Abstract:

Objective To explore the variance in gene profiles of hypothalamus in SHR by acupuncture at Renying(ST9)from the perspective of gene chip. Methods Twenty Spontaneously Hypertensive Rats(SHR)were randomly divided into Renying(ST9)group and model control group, and ten WKY rats were used as blank group. The acupuncture intervention was applied to Renying group everyday. The model group and normal group rats received no treatment. The blood pressure was measured every four days, the experiment lasted for 28 days. Gene microarray was performed after completion of the study. Results There was no significant change in the blood pressure of the blank group, and the blood pressure of the model group was significantly higher than that of the blank group(P<0.01). Compared with model control group, blood pressure of Renying(ST9)group did not drop to normal, but the blood pressure was remarkably decreased(P<0.05), the difference was statistically significant(P<0.01). Gene microarray results showed differences in gene expression between different groups. Compared with the blank group, there were 129 genes up-regulated and 243 genes down-regulated of the model group; compared with the model group, there were 167 genes up-regulated and 188 genes down regulated of Renying(ST9)group. Conclusion Acupuncture at Renying(ST9)has a significant hypotensive effect on SHR, and has a certain effect on the gene expression of hypothalamus in rats, and its mechanism may be related to the antihypertensive principle of acupuncture.

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备注/Memo

备注/Memo:

基金项目: 国家中医药管理局专项(201507001-08)
作者简介: 郭妍(1987- ),女,博士,住院医师。研究方向:针刺的临床与效应研究。E-mail:guoyan7777777@126.com
通信作者: 石学敏(1938- ),本科,教授,主任医师,中国工程院院士,博士生导师。研究方向:针刺的临床与效应研究。E-mail:shixuemin56789@163.com.

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更新日期/Last Update: 2017-11-06