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[1]郭子宁,张立平,黄象安,等.扶正祛毒方对慢性乙肝病毒携带者血IFN-γ表达影响的研究[J].环球中医药,2017,10(12):1447-1449.[doi:10.3969/j.issn.1674-1749.2017.12.007]
　GUO Zining,ZHANG Liping,HUANG Xiangan,et al.Effect of Fuzheng Qudu formula on the expression of serum IFN-γ in chronic hepatitis B carriers[J].,2017,10(12):1447-1449.[doi:10.3969/j.issn.1674-1749.2017.12.007]
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扶正祛毒方对慢性乙肝病毒携带者血IFN-γ表达影响的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年12期
页码:
1447-1449
栏目:
论著
出版日期:
2017-12-06

文章信息/Info

Title:
Effect of Fuzheng Qudu formula on the expression of serum IFN-γ in chronic hepatitis B carriers
作者:
郭子宁; 张立平; 黄象安; 彭龙; 金艳
100078 北京中医药大学东方医院感染科(郭子宁、黄象安); 北京中医药大学研究生院[张立平、彭龙(硕士研究生)、金艳(硕士研究生)]
Author(s):
GUO Zining;  ZHANG Liping;  HUANG Xiangan;  et al.
Dongfang Hospital of Beijing University of Traditional Chinese Medicine, Beijing University of Traditional Chinese Medicine, Beijing 100078,China
关键词:
慢性乙肝病毒携带者;  扶正祛毒方;  免疫调节;  干扰素-γ
Keywords:
Chronic hepatitis B carriers;  Fuzheng Qudu formula;  Immunoregulatory;  IFN-γ
分类号:
R575.1
DOI:
10.3969/j.issn.1674-1749.2017.12.007
文献标志码:
A
摘要:
目的 观察扶正祛毒方对慢性乙肝病毒携带者血IFN-γ表达的影响,探讨扶正祛毒方对慢性乙肝病毒携带者的免疫调节作用。方法 对慢性乙肝病毒携带者给予扶正祛毒方口服干预12周,于入组第1天和第12周分别采血取血清,RT-PCR法检测HBV DNA滴度,生化法测ALT、AST水平,ELISA测HBeAg滴度、细胞因子IFN-γ表达。结果 扶正祛毒方治疗12周后,慢性乙肝病毒携带者血ALT、AST水平均在正常范围; 慢性乙肝病毒携带者血HBV DNA滴度较入组第1天下降,差异显著(P<0.05); HBeAg滴度较入组第1天下降,差异显著(P<0.05); 细胞因子IFN-γ表达较入组第1天上升,差异显著(P<0.05)。结论(1)用药12周后,扶正祛毒方对慢乙肝病毒携带者肝脏炎症反应无加剧,作用安全;(2)用药12周后,扶正祛毒方对慢性乙肝病毒携带者病毒复制有抑制作用;(3)用药12周后,扶正祛毒方可以提高慢性乙肝病毒携带者血清IFN-γ表达,这可能是其抑制病毒复制的重要机制之一。
Abstract:
Objective To observe the effect of Fuzheng Qudu formula on the expression of serum IFN-γ in chronic hepatitis B carriers,and to discuss the immunoregulatory effects of Fuzheng Qudu formula in chronic hepatitis B carriers. Methods Chronic hepatitis B carriers were treated with Fuzheng Qudu formula orally for 12 weeks. The serum was collected at the first day and the last day. The expression of HBV DNA in serum was detected by reverse transcription-polymerase chain reaction method. The level of ALT and AST in serum was detected by biochemical process. The expression of HBeAg, and serum IFN-γ was detected by enzyme-linked immune sorbent assay method. Results After 12 weeks treatment with Fuzheng Qudu formula, the levels of serum ALT and AST were normal in chronic hepatitis B carriers; The expression of HBV DNA in serum was lower than that of the first day began the treatment, and the difference was significant(P<0.05).The expression of HBeAg in serum was lower than the first day began the treatment, and the difference was significant(P<0.05).The expression of IFN-γ in serum was higher than the first day began the treatment, and the difference was significant(P<0.05). Conclusion(1)After 12 weeks of treatment, Fuzheng Qudu formula have no side effect on liver inflammation in Chronic hepatitis B carriers, the action is safe.(2)After 12 weeks of treatment, Fuzheng Qudu formula has inhibitory effect on viral replication of chronic hepatitis B virus carriers.(3)After 12 weeks of treatment, Fuzheng Qudu formula can improve the expression of serum IFN- γ in chronic hepatitis B virus carriers, which may be one of the important mechanisms of inhibiting viral replication.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金青年基金(81503473); 北京中医药大学基本科研业务项目(2015-JYB-JSMS112)
作者简介: 郭子宁(1983- ),女,硕士,主治医师。研究方向:慢性肝炎、肝纤维化的中西医治疗。E-mail:guozning@126.com
通信作者:张立平(1960- ),女,博士,教授。研究方向:慢性消化系统疾病以及代谢综合征的中西医治疗。E-mail:lpzhang2005@126.com
更新日期/Last Update: 2017-11-06