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[1]程钱,赵崇军,代一航,等.不同产地山豆根UPLC指纹图谱的研究[J].环球中医药,2017,10(12):1450-1455.[doi:10.3969/j.issn.1674-1749.2017.12.008]
　CHENG Qian,ZHAO Chongjun,DAI Yihang,et al.Study of the UPLC fingerprint of sophorae tonkinensis radix et rhizoma from different regions[J].,2017,10(12):1450-1455.[doi:10.3969/j.issn.1674-1749.2017.12.008]
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不同产地山豆根UPLC指纹图谱的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年12期
页码:
1450-1455
栏目:
论著
出版日期:
2017-12-06

文章信息/Info

Title:
Study of the UPLC fingerprint of sophorae tonkinensis radix et rhizoma from different regions
作者:
程钱; 赵崇军; 代一航; 汪建芬; 夏青; 张文婷; 王金凤; 马志强; 林瑞超
100102 北京中医药大学中药学院[程钱(硕士研究生)、赵崇军(博士研究生)、代一航(硕士研究生)、汪建芬(硕士研究生)、夏青(博士研究生)、张文婷(博士研究生)、王金凤(博士研究生)、马志强、林瑞超]
Author(s):
CHENG Qian;  ZHAO Chongjun;  DAI Yihang;  et al.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing Key Laboratory for Quality Evaluation of Traditional Chinese Medicine,Beijing 100102, China
关键词:
山豆根;  超高效液相色谱;  指纹图谱
Keywords:
Sophorae tonkinensis radix et rhizoma;  Ultra-high performance liquid chromatography;  Fingerprint
分类号:
R284.1
DOI:
10.3969/j.issn.1674-1749.2017.12.008
文献标志码:
A
摘要:
目的 建立山豆根的UPLC指纹图谱分析方法,为山豆根的质量控制和质量评价提供参考。方法 采用超高效液相色谱Waters UPLC H-Class,Agilent Zorbax SB C-18 RRHD色谱柱(2.1×100 mm,1.8 μm),预柱:Agilent SB-C18色谱柱(2.1×5 mm,1.8 μm),流动相为乙腈-0.2%磷酸溶液梯度洗脱,洗脱程序0～5分钟,5%A; 5～6分钟,5%～9%A; 6～6.5分钟,9%～12%A; 6.5～11分钟,12%～15%A; 11～21分钟,15%～34%A; 21～23分钟,34%～45%A; 23～26分钟,45%～47%A; 26～27分钟,47%～77%A; 27～35分钟,77%～98%A,流速0.3 mL·min-1,检测波长215 nm,柱温30℃,采用《中药色谱指纹图谱相似度评价系统》2004A版软件进行数据处理。 结果 建立的山豆根指纹图谱有27个共有峰,指认了2个共有峰; 各批药材指纹图谱比较相似度大部分在0.90以上,符合指纹图谱研究技术的要求,说明本研究中的不同产地的山豆根在化学成分上相似度良好。 结论 该方法精密度、稳定性及重复性较好,特征性及专属性较强,可以有效地对山豆根药材进行质量控制,同时可以对山豆根和北豆根的指纹图谱的比较提供思路,从而避免因临床混用导致的药物中毒事件的发生。
Abstract:
Objective To establish the UPLC fingerprint methods of sophorae tonkinensis radix et rhizoma and provide reference for quality evaluation and quality control of sophorae tonkinensis radix et rhizoma. Methods The UPLC method was used. An Agilent Zorbax SB C-18 RRHD(2.1×100 mm,1.8 μm)column was adopted and the mobile phase consisted of acetonitrile and 0.2% phosphoric acid solution with a gradient elution. The elution program:0～5min,5% A; 5～6min,5%～9% A; 6～6.5min,9%～12% A; 6.5～11min,12%～15% A; 11～21min,15%～34% A; 21～23min,34%～45% A; 23～26min,45%～47%A; 26～27min,47%～77%A; 27～35min,77%～98% A.The flow rate was 0.3 mL·min-1, column temperature was 30℃ and detection wavelength was 215 nm. A software “Similarity Evaluation System for Chromatographic Fingerprint of TCM” 2004A was used for data processing. Results The establishment fingerprint of sophorae tonkinensis radix et rhizoma has 27 common peaks and 2 peaks of them were identified. The similarity of fingerprints of each batch was above 0.90 which was in line with the requirements of fingerprint technology research.So it proved that the composition of sophorae tonkinensis radix et rhizoma from different habitats in this research had good similarity. Conclusion The precision, stability and reproducibility of the established method were good, and the characterization and specificity is strong. The method could be effectively used for quality control of Sophorae Tonkinensis Radix et Rhizoma herb, at the same time, it can provide idea to distinguish Sophorae Tonkinensis Radix et Rhizoma and Menispermi Rhizoma, so as to help avoid the occurrence of drug poisoning caused by clinical mixing.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 2015年公益性行业科研专项(201507002)
作者简介: 程钱(1992- ),2014级在读硕士研究生。研究方向:中药检验与分析。E-mail:chengq_0713@163.com
通信作者: 林瑞超(1956- ),博士,教授,博士生导师。研究方向:天然药物化学、民族药品质评价。E-mail:linrch307@sina.com
更新日期/Last Update: 2017-11-06